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Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors
Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated hi...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group US
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484786/ https://www.ncbi.nlm.nih.gov/pubmed/37640880 http://dx.doi.org/10.1038/s41588-023-01486-9 |
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author | Lin, Xiao Jia, Yuxin Heal, Robert Prokchorchik, Maxim Sindalovskaya, Maria Olave-Achury, Andrea Makechemu, Moffat Fairhead, Sebastian Noureen, Azka Heo, Jung Witek, Kamil Smoker, Matthew Taylor, Jodie Shrestha, Ram-Krishna Lee, Yoonyoung Zhang, Chunzhi Park, Soon Ju Sohn, Kee Hoon Huang, Sanwen Jones, Jonathan D. G. |
author_facet | Lin, Xiao Jia, Yuxin Heal, Robert Prokchorchik, Maxim Sindalovskaya, Maria Olave-Achury, Andrea Makechemu, Moffat Fairhead, Sebastian Noureen, Azka Heo, Jung Witek, Kamil Smoker, Matthew Taylor, Jodie Shrestha, Ram-Krishna Lee, Yoonyoung Zhang, Chunzhi Park, Soon Ju Sohn, Kee Hoon Huang, Sanwen Jones, Jonathan D. G. |
author_sort | Lin, Xiao |
collection | PubMed |
description | Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize cognate P. infestans RXLR effectors PITG_22825 (AVRamr4), PITG_02860 and PITG_04373. These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops. |
format | Online Article Text |
id | pubmed-10484786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group US |
record_format | MEDLINE/PubMed |
spelling | pubmed-104847862023-09-09 Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors Lin, Xiao Jia, Yuxin Heal, Robert Prokchorchik, Maxim Sindalovskaya, Maria Olave-Achury, Andrea Makechemu, Moffat Fairhead, Sebastian Noureen, Azka Heo, Jung Witek, Kamil Smoker, Matthew Taylor, Jodie Shrestha, Ram-Krishna Lee, Yoonyoung Zhang, Chunzhi Park, Soon Ju Sohn, Kee Hoon Huang, Sanwen Jones, Jonathan D. G. Nat Genet Article Potato (Solanum tuberosum) and tomato (Solanum lycopersicon) crops suffer severe losses to late blight caused by the oomycete pathogen Phytophthora infestans. Solanum americanum, a relative of potato and tomato, is globally distributed and most accessions are highly blight resistant. We generated high-quality reference genomes of four S. americanum accessions, resequenced 52 accessions, and defined a pan-NLRome of S. americanum immune receptor genes. We further screened for variation in recognition of 315P. infestans RXLR effectors in 52 S. americanum accessions. Using these genomic and phenotypic data, we cloned three NLR-encoding genes, Rpi-amr4, R02860 and R04373, that recognize cognate P. infestans RXLR effectors PITG_22825 (AVRamr4), PITG_02860 and PITG_04373. These genomic resources and methodologies will support efforts to engineer potatoes with durable late blight resistance and can be applied to diseases of other crops. Nature Publishing Group US 2023-08-28 2023 /pmc/articles/PMC10484786/ /pubmed/37640880 http://dx.doi.org/10.1038/s41588-023-01486-9 Text en © The Author(s) 2023, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Lin, Xiao Jia, Yuxin Heal, Robert Prokchorchik, Maxim Sindalovskaya, Maria Olave-Achury, Andrea Makechemu, Moffat Fairhead, Sebastian Noureen, Azka Heo, Jung Witek, Kamil Smoker, Matthew Taylor, Jodie Shrestha, Ram-Krishna Lee, Yoonyoung Zhang, Chunzhi Park, Soon Ju Sohn, Kee Hoon Huang, Sanwen Jones, Jonathan D. G. Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
title | Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
title_full | Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
title_fullStr | Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
title_full_unstemmed | Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
title_short | Solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
title_sort | solanum americanum genome-assisted discovery of immune receptors that detect potato late blight pathogen effectors |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484786/ https://www.ncbi.nlm.nih.gov/pubmed/37640880 http://dx.doi.org/10.1038/s41588-023-01486-9 |
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