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Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line
Canine Soft Tissue Sarcoma (STS) cell line A-72 has been largely employed for antiviral and antiproliferative studies. However, there are few information on their characteristics. Our aim was to evaluate A-72 expression level of genes and proteins involved in the innate immune response and cell cycl...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Netherlands
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484808/ https://www.ncbi.nlm.nih.gov/pubmed/37038001 http://dx.doi.org/10.1007/s11259-023-10115-z |
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author | Razzuoli, Elisabetta Chirullo, Barbara De Ciucis, Chiara Grazia Mecocci, Samanta Martini, Isabella Zoccola, Roberto Campanella, Chiara Varello, Katia Petrucci, Paola Di Meo, Antonio Bozzetta, Elena Tarantino, Michela Goria, Maria Modesto, Paola |
author_facet | Razzuoli, Elisabetta Chirullo, Barbara De Ciucis, Chiara Grazia Mecocci, Samanta Martini, Isabella Zoccola, Roberto Campanella, Chiara Varello, Katia Petrucci, Paola Di Meo, Antonio Bozzetta, Elena Tarantino, Michela Goria, Maria Modesto, Paola |
author_sort | Razzuoli, Elisabetta |
collection | PubMed |
description | Canine Soft Tissue Sarcoma (STS) cell line A-72 has been largely employed for antiviral and antiproliferative studies. However, there are few information on their characteristics. Our aim was to evaluate A-72 expression level of genes and proteins involved in the innate immune response and cell cycle, their ability to respond to infective stressors and their possible use as a cellular model for anti-cancer studies in human and animal medicine. For this purpose, we evaluated the basal expression of immune-related, cell cycle and DNA repair genes on this cell line and tumoral tissues. A-72 ability to respond to a wild-type strain of Salmonella typhimurium was assessed. S. typhimurium showed ability to penetrate A-72 causing pro-inflammatory response accompanied by a decrease of cell viability. IL10 and IL18 genes were not expressed in A-72 while CXCL8, NOS2, CXCR4 and PTEN were highly expressed in all samples and TP53 was slightly expressed, as shown in human STS. Our results outline the ability of A-72 to respond to a bacterial agent by modifying the expression of important genes involved in innate immune response and provide a useful model for in vitro evaluation of new therapeutic approaches that could be translated into the human oncology. |
format | Online Article Text |
id | pubmed-10484808 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Netherlands |
record_format | MEDLINE/PubMed |
spelling | pubmed-104848082023-09-09 Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line Razzuoli, Elisabetta Chirullo, Barbara De Ciucis, Chiara Grazia Mecocci, Samanta Martini, Isabella Zoccola, Roberto Campanella, Chiara Varello, Katia Petrucci, Paola Di Meo, Antonio Bozzetta, Elena Tarantino, Michela Goria, Maria Modesto, Paola Vet Res Commun Research Canine Soft Tissue Sarcoma (STS) cell line A-72 has been largely employed for antiviral and antiproliferative studies. However, there are few information on their characteristics. Our aim was to evaluate A-72 expression level of genes and proteins involved in the innate immune response and cell cycle, their ability to respond to infective stressors and their possible use as a cellular model for anti-cancer studies in human and animal medicine. For this purpose, we evaluated the basal expression of immune-related, cell cycle and DNA repair genes on this cell line and tumoral tissues. A-72 ability to respond to a wild-type strain of Salmonella typhimurium was assessed. S. typhimurium showed ability to penetrate A-72 causing pro-inflammatory response accompanied by a decrease of cell viability. IL10 and IL18 genes were not expressed in A-72 while CXCL8, NOS2, CXCR4 and PTEN were highly expressed in all samples and TP53 was slightly expressed, as shown in human STS. Our results outline the ability of A-72 to respond to a bacterial agent by modifying the expression of important genes involved in innate immune response and provide a useful model for in vitro evaluation of new therapeutic approaches that could be translated into the human oncology. Springer Netherlands 2023-04-11 2023 /pmc/articles/PMC10484808/ /pubmed/37038001 http://dx.doi.org/10.1007/s11259-023-10115-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Razzuoli, Elisabetta Chirullo, Barbara De Ciucis, Chiara Grazia Mecocci, Samanta Martini, Isabella Zoccola, Roberto Campanella, Chiara Varello, Katia Petrucci, Paola Di Meo, Antonio Bozzetta, Elena Tarantino, Michela Goria, Maria Modesto, Paola Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line |
title | Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line |
title_full | Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line |
title_fullStr | Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line |
title_full_unstemmed | Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line |
title_short | Animal models of Soft Tissue Sarcoma for alternative anticancer therapy studies: characterization of the A-72 Canine Cell Line |
title_sort | animal models of soft tissue sarcoma for alternative anticancer therapy studies: characterization of the a-72 canine cell line |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484808/ https://www.ncbi.nlm.nih.gov/pubmed/37038001 http://dx.doi.org/10.1007/s11259-023-10115-z |
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