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Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance

Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, and white chick syndrome. In the current study, we aimed to evaluate the effect of CAstV infection on growth, performance, and gross and histopathological picture of commercial chicken flocks suffering inc...

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Autores principales: Sallam, Ahmed A., Al-Mokaddem, Asmaa K., Hamoud, Mohamed M., Samir, Mahmoud, Khalifa, Rabab Amin, Abdelgayed, Sherein S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484817/
https://www.ncbi.nlm.nih.gov/pubmed/36976444
http://dx.doi.org/10.1007/s11259-023-10109-x
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author Sallam, Ahmed A.
Al-Mokaddem, Asmaa K.
Hamoud, Mohamed M.
Samir, Mahmoud
Khalifa, Rabab Amin
Abdelgayed, Sherein S.
author_facet Sallam, Ahmed A.
Al-Mokaddem, Asmaa K.
Hamoud, Mohamed M.
Samir, Mahmoud
Khalifa, Rabab Amin
Abdelgayed, Sherein S.
author_sort Sallam, Ahmed A.
collection PubMed
description Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, and white chick syndrome. In the current study, we aimed to evaluate the effect of CAstV infection on growth, performance, and gross and histopathological picture of commercial chicken flocks suffering increased culling rate and decreased performance. Samples were collected for virus isolation, identification, and sequencing on day one, 15 days, and 30 days of age. Body weight, feed conversion rate, and mortality rates were determined. A gross examination was performed, and tissue samples from the liver, intestine, kidneys, heart, and lungs were kept in formalin for histopathological evaluation. Embryos inoculated with CAstV revealed dwarfism, and edema. The cytopathic effect on CAstV inoculated cells included aggregation,, and sloughing. The isolated Egyptian isolates shared the highest nucleotide homology (93%) with the Korean isolate Kr/ADL102655-1/2010 and showed the most distant relation to the Indian isolate Indovax/APF/1319 with 82–83% homology. Body weight exhibited significant reduction with a decrease in feed conversion rate in CAstV infected flocks. Gross examination of CAstV-infected chickens revealed white feathered chicks on day one, and poor body condition in older chickens as well as swollen kidneys. Histopathological examination of CAstV-infected birds showed mild proventriculitis, shortening of intestinal villi, enteritis, focal hepatocellular necrosis, pericarditis, myocarditis, and proliferative response in lung tissue. Kidneys showed interstitial nephritis, urate deposition, and glomerular hypercellularity. CAstV is a chicken pathogen that could be related to decreased performance, and screening of flocks for CAstV might be an essential step for breeders. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11259-023-10109-x.
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spelling pubmed-104848172023-09-09 Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance Sallam, Ahmed A. Al-Mokaddem, Asmaa K. Hamoud, Mohamed M. Samir, Mahmoud Khalifa, Rabab Amin Abdelgayed, Sherein S. Vet Res Commun Research Chicken astroviruses (CAstV) were associated with retarded growth, enteritis, kidney diseases, and white chick syndrome. In the current study, we aimed to evaluate the effect of CAstV infection on growth, performance, and gross and histopathological picture of commercial chicken flocks suffering increased culling rate and decreased performance. Samples were collected for virus isolation, identification, and sequencing on day one, 15 days, and 30 days of age. Body weight, feed conversion rate, and mortality rates were determined. A gross examination was performed, and tissue samples from the liver, intestine, kidneys, heart, and lungs were kept in formalin for histopathological evaluation. Embryos inoculated with CAstV revealed dwarfism, and edema. The cytopathic effect on CAstV inoculated cells included aggregation,, and sloughing. The isolated Egyptian isolates shared the highest nucleotide homology (93%) with the Korean isolate Kr/ADL102655-1/2010 and showed the most distant relation to the Indian isolate Indovax/APF/1319 with 82–83% homology. Body weight exhibited significant reduction with a decrease in feed conversion rate in CAstV infected flocks. Gross examination of CAstV-infected chickens revealed white feathered chicks on day one, and poor body condition in older chickens as well as swollen kidneys. Histopathological examination of CAstV-infected birds showed mild proventriculitis, shortening of intestinal villi, enteritis, focal hepatocellular necrosis, pericarditis, myocarditis, and proliferative response in lung tissue. Kidneys showed interstitial nephritis, urate deposition, and glomerular hypercellularity. CAstV is a chicken pathogen that could be related to decreased performance, and screening of flocks for CAstV might be an essential step for breeders. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11259-023-10109-x. Springer Netherlands 2023-03-28 2023 /pmc/articles/PMC10484817/ /pubmed/36976444 http://dx.doi.org/10.1007/s11259-023-10109-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Sallam, Ahmed A.
Al-Mokaddem, Asmaa K.
Hamoud, Mohamed M.
Samir, Mahmoud
Khalifa, Rabab Amin
Abdelgayed, Sherein S.
Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
title Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
title_full Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
title_fullStr Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
title_full_unstemmed Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
title_short Chicken Astro virus (CAstV): Isolation and characterization of new strains in broiler flocks with poor performance
title_sort chicken astro virus (castv): isolation and characterization of new strains in broiler flocks with poor performance
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484817/
https://www.ncbi.nlm.nih.gov/pubmed/36976444
http://dx.doi.org/10.1007/s11259-023-10109-x
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