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A generalizable Cas9/sgRNA prediction model using machine transfer learning with small high-quality datasets

The CRISPR/Cas9 nuclease from Streptococcus pyogenes (SpCas9) can be used with single guide RNAs (sgRNAs) as a sequence-specific antimicrobial agent and as a genome-engineering tool. However, current bacterial sgRNA activity models struggle with accurate predictions and do not generalize well, possi...

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Detalles Bibliográficos
Autores principales:	Ham, Dalton T., Browne, Tyler S., Banglorewala, Pooja N., Wilson, Tyler L., Michael, Richard K., Gloor, Gregory B., Edgell, David R.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485023/ https://www.ncbi.nlm.nih.gov/pubmed/37679324 http://dx.doi.org/10.1038/s41467-023-41143-7

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