Cargando…
A system for bioelectronic delivery of treatment directed toward wound healing
The development of wearable bioelectronic systems is a promising approach for optimal delivery of therapeutic treatments. These systems can provide continuous delivery of ions, charged biomolecules, and an electric field for various medical applications. However, rapid prototyping of wearable bioele...
Autores principales: | , , , , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485133/ https://www.ncbi.nlm.nih.gov/pubmed/37679425 http://dx.doi.org/10.1038/s41598-023-41572-w |
_version_ | 1785102727802519552 |
---|---|
author | Baniya, Prabhat Tebyani, Maryam Asefifeyzabadi, Narges Nguyen, Tiffany Hernandez, Cristian Zhu, Kan Li, Houpu Selberg, John Hsieh, Hao-Chieh Pansodtee, Pattawong Yang, Hsin-ya Recendez, Cynthia Keller, Gordon Hee, Wan Shen Aslankoohi, Elham Isseroff, Roslyn Rivkah Zhao, Min Gomez, Marcella Rolandi, Marco Teodorescu, Mircea |
author_facet | Baniya, Prabhat Tebyani, Maryam Asefifeyzabadi, Narges Nguyen, Tiffany Hernandez, Cristian Zhu, Kan Li, Houpu Selberg, John Hsieh, Hao-Chieh Pansodtee, Pattawong Yang, Hsin-ya Recendez, Cynthia Keller, Gordon Hee, Wan Shen Aslankoohi, Elham Isseroff, Roslyn Rivkah Zhao, Min Gomez, Marcella Rolandi, Marco Teodorescu, Mircea |
author_sort | Baniya, Prabhat |
collection | PubMed |
description | The development of wearable bioelectronic systems is a promising approach for optimal delivery of therapeutic treatments. These systems can provide continuous delivery of ions, charged biomolecules, and an electric field for various medical applications. However, rapid prototyping of wearable bioelectronic systems for controlled delivery of specific treatments with a scalable fabrication process is challenging. We present a wearable bioelectronic system comprised of a polydimethylsiloxane (PDMS) device cast in customizable 3D printed molds and a printed circuit board (PCB), which employs commercially available engineering components and tools throughout design and fabrication. The system, featuring solution-filled reservoirs, embedded electrodes, and hydrogel-filled capillary tubing, is assembled modularly. The PDMS and PCB both contain matching through-holes designed to hold metallic contact posts coated with silver epoxy, allowing for mechanical and electrical integration. This assembly scheme allows us to interchange subsystem components, such as various PCB designs and reservoir solutions. We present three PCB designs: a wired version and two battery-powered versions with and without onboard memory. The wired design uses an external voltage controller for device actuation. The battery-powered PCB design uses a microcontroller unit to enable pre-programmed applied voltages and deep sleep mode to prolong battery run time. Finally, the battery-powered PCB with onboard memory is developed to record delivered currents, which enables us to verify treatment dose delivered. To demonstrate the functionality of the platform, the devices are used to deliver H[Formula: see text] in vivo using mouse models and fluoxetine ex vivo using a simulated wound environment. Immunohistochemistry staining shows an improvement of 35.86% in the M1/M2 ratio of H[Formula: see text] —treated wounds compared with control wounds, indicating the potential of the platform to improve wound healing. |
format | Online Article Text |
id | pubmed-10485133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-104851332023-09-09 A system for bioelectronic delivery of treatment directed toward wound healing Baniya, Prabhat Tebyani, Maryam Asefifeyzabadi, Narges Nguyen, Tiffany Hernandez, Cristian Zhu, Kan Li, Houpu Selberg, John Hsieh, Hao-Chieh Pansodtee, Pattawong Yang, Hsin-ya Recendez, Cynthia Keller, Gordon Hee, Wan Shen Aslankoohi, Elham Isseroff, Roslyn Rivkah Zhao, Min Gomez, Marcella Rolandi, Marco Teodorescu, Mircea Sci Rep Article The development of wearable bioelectronic systems is a promising approach for optimal delivery of therapeutic treatments. These systems can provide continuous delivery of ions, charged biomolecules, and an electric field for various medical applications. However, rapid prototyping of wearable bioelectronic systems for controlled delivery of specific treatments with a scalable fabrication process is challenging. We present a wearable bioelectronic system comprised of a polydimethylsiloxane (PDMS) device cast in customizable 3D printed molds and a printed circuit board (PCB), which employs commercially available engineering components and tools throughout design and fabrication. The system, featuring solution-filled reservoirs, embedded electrodes, and hydrogel-filled capillary tubing, is assembled modularly. The PDMS and PCB both contain matching through-holes designed to hold metallic contact posts coated with silver epoxy, allowing for mechanical and electrical integration. This assembly scheme allows us to interchange subsystem components, such as various PCB designs and reservoir solutions. We present three PCB designs: a wired version and two battery-powered versions with and without onboard memory. The wired design uses an external voltage controller for device actuation. The battery-powered PCB design uses a microcontroller unit to enable pre-programmed applied voltages and deep sleep mode to prolong battery run time. Finally, the battery-powered PCB with onboard memory is developed to record delivered currents, which enables us to verify treatment dose delivered. To demonstrate the functionality of the platform, the devices are used to deliver H[Formula: see text] in vivo using mouse models and fluoxetine ex vivo using a simulated wound environment. Immunohistochemistry staining shows an improvement of 35.86% in the M1/M2 ratio of H[Formula: see text] —treated wounds compared with control wounds, indicating the potential of the platform to improve wound healing. Nature Publishing Group UK 2023-09-07 /pmc/articles/PMC10485133/ /pubmed/37679425 http://dx.doi.org/10.1038/s41598-023-41572-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Baniya, Prabhat Tebyani, Maryam Asefifeyzabadi, Narges Nguyen, Tiffany Hernandez, Cristian Zhu, Kan Li, Houpu Selberg, John Hsieh, Hao-Chieh Pansodtee, Pattawong Yang, Hsin-ya Recendez, Cynthia Keller, Gordon Hee, Wan Shen Aslankoohi, Elham Isseroff, Roslyn Rivkah Zhao, Min Gomez, Marcella Rolandi, Marco Teodorescu, Mircea A system for bioelectronic delivery of treatment directed toward wound healing |
title | A system for bioelectronic delivery of treatment directed toward wound healing |
title_full | A system for bioelectronic delivery of treatment directed toward wound healing |
title_fullStr | A system for bioelectronic delivery of treatment directed toward wound healing |
title_full_unstemmed | A system for bioelectronic delivery of treatment directed toward wound healing |
title_short | A system for bioelectronic delivery of treatment directed toward wound healing |
title_sort | system for bioelectronic delivery of treatment directed toward wound healing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485133/ https://www.ncbi.nlm.nih.gov/pubmed/37679425 http://dx.doi.org/10.1038/s41598-023-41572-w |
work_keys_str_mv | AT baniyaprabhat asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT tebyanimaryam asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT asefifeyzabadinarges asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT nguyentiffany asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT hernandezcristian asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT zhukan asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT lihoupu asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT selbergjohn asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT hsiehhaochieh asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT pansodteepattawong asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT yanghsinya asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT recendezcynthia asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT kellergordon asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT heewanshen asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT aslankoohielham asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT isseroffroslynrivkah asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT zhaomin asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT gomezmarcella asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT rolandimarco asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT teodorescumircea asystemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT baniyaprabhat systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT tebyanimaryam systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT asefifeyzabadinarges systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT nguyentiffany systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT hernandezcristian systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT zhukan systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT lihoupu systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT selbergjohn systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT hsiehhaochieh systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT pansodteepattawong systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT yanghsinya systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT recendezcynthia systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT kellergordon systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT heewanshen systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT aslankoohielham systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT isseroffroslynrivkah systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT zhaomin systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT gomezmarcella systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT rolandimarco systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing AT teodorescumircea systemforbioelectronicdeliveryoftreatmentdirectedtowardwoundhealing |