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Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
INTRODUCTION: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic fe...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485380/ https://www.ncbi.nlm.nih.gov/pubmed/37694115 http://dx.doi.org/10.3389/fnins.2023.1236049 |
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author | Omar, Noor Azzizah Kumar, Jaya Teoh, Seong Lin |
author_facet | Omar, Noor Azzizah Kumar, Jaya Teoh, Seong Lin |
author_sort | Omar, Noor Azzizah |
collection | PubMed |
description | INTRODUCTION: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish. METHODS: Fish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection. RESULTS: Between one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment. DISCUSSION: Over a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies. |
format | Online Article Text |
id | pubmed-10485380 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-104853802023-09-09 Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection Omar, Noor Azzizah Kumar, Jaya Teoh, Seong Lin Front Neurosci Neuroscience INTRODUCTION: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish. METHODS: Fish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection. RESULTS: Between one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment. DISCUSSION: Over a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies. Frontiers Media S.A. 2023-08-25 /pmc/articles/PMC10485380/ /pubmed/37694115 http://dx.doi.org/10.3389/fnins.2023.1236049 Text en Copyright © 2023 Omar, Kumar and Teoh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Omar, Noor Azzizah Kumar, Jaya Teoh, Seong Lin Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection |
title | Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection |
title_full | Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection |
title_fullStr | Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection |
title_full_unstemmed | Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection |
title_short | Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection |
title_sort | parkinson’s disease model in zebrafish using intraperitoneal mptp injection |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485380/ https://www.ncbi.nlm.nih.gov/pubmed/37694115 http://dx.doi.org/10.3389/fnins.2023.1236049 |
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