Cargando…

Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

INTRODUCTION: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic fe...

Descripción completa

Detalles Bibliográficos
Autores principales: Omar, Noor Azzizah, Kumar, Jaya, Teoh, Seong Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485380/
https://www.ncbi.nlm.nih.gov/pubmed/37694115
http://dx.doi.org/10.3389/fnins.2023.1236049
_version_ 1785102772447739904
author Omar, Noor Azzizah
Kumar, Jaya
Teoh, Seong Lin
author_facet Omar, Noor Azzizah
Kumar, Jaya
Teoh, Seong Lin
author_sort Omar, Noor Azzizah
collection PubMed
description INTRODUCTION: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish. METHODS: Fish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection. RESULTS: Between one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment. DISCUSSION: Over a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies.
format Online
Article
Text
id pubmed-10485380
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-104853802023-09-09 Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection Omar, Noor Azzizah Kumar, Jaya Teoh, Seong Lin Front Neurosci Neuroscience INTRODUCTION: Parkinson’s disease (PD) is the second most common neurodegenerative disease that severely affects the quality of life of patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown to reflect behavioral, molecular, and proteomic features of PD. This study aimed to assess the protocol for inducing PD following MPTP injection in adult zebrafish. METHODS: Fish were injected with 100 μg/g of MPTP intraperitoneally once or twice and then assessed on days 1 to 30 post-injection. RESULTS: Between one-time and two-time injections, there was no significant difference in most locomotor parameters, expressions of tyrosine hydroxylase-2 (th2) and dopamine transporter (dat) genes, and dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- and two-time injections on the day 1 assessment. DISCUSSION: Over a 30-day period, the parameters showed significant differences in swimming speed, total distance traveled, tyrosine hydroxylase-1 (th1) and dat gene expressions, caspase-3 and glutathione protein levels, and TH+ cell counts. Days 3 and 5 showed the most changes compared to the control. In conclusion, a one-time injection of MPTP with delayed assessment on days 3 to 5 is a good PD model for animal studies. Frontiers Media S.A. 2023-08-25 /pmc/articles/PMC10485380/ /pubmed/37694115 http://dx.doi.org/10.3389/fnins.2023.1236049 Text en Copyright © 2023 Omar, Kumar and Teoh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Omar, Noor Azzizah
Kumar, Jaya
Teoh, Seong Lin
Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
title Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
title_full Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
title_fullStr Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
title_full_unstemmed Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
title_short Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection
title_sort parkinson’s disease model in zebrafish using intraperitoneal mptp injection
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485380/
https://www.ncbi.nlm.nih.gov/pubmed/37694115
http://dx.doi.org/10.3389/fnins.2023.1236049
work_keys_str_mv AT omarnoorazzizah parkinsonsdiseasemodelinzebrafishusingintraperitonealmptpinjection
AT kumarjaya parkinsonsdiseasemodelinzebrafishusingintraperitonealmptpinjection
AT teohseonglin parkinsonsdiseasemodelinzebrafishusingintraperitonealmptpinjection