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To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver
AIM: To evaluate the antimicrobial activity of PRP and PRF with and without nanosilver. MATERIALS AND METHODS: The materials were tested in powdered form is nanosilver. The nanosilver particles was mixed to form with PRP and PRF so as to placed in a wells followed the groups are experimental groups;...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485451/ https://www.ncbi.nlm.nih.gov/pubmed/37694058 http://dx.doi.org/10.4103/jpbs.jpbs_72_23 |
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author | Zafar, Adil Tripathi, Vartika Khan, Mariyam Manglam, Keshav K. Rastogi, Preeti Almotreb, Abdullah M. |
author_facet | Zafar, Adil Tripathi, Vartika Khan, Mariyam Manglam, Keshav K. Rastogi, Preeti Almotreb, Abdullah M. |
author_sort | Zafar, Adil |
collection | PubMed |
description | AIM: To evaluate the antimicrobial activity of PRP and PRF with and without nanosilver. MATERIALS AND METHODS: The materials were tested in powdered form is nanosilver. The nanosilver particles was mixed to form with PRP and PRF so as to placed in a wells followed the groups are experimental groups; Group I: PRP + nanosilver particles, Group II: PRF + nanosilver and control group: PRP and PRF and normal saline. Silver nanoparticles was tested at concentrations of 50 μ gram per mL. The powder was prepared for each group with identical amount of the powder (milligram/mg) and then mixed with 1 milliliter liquid. The plates are then incubated at 37°C under appropriate atmospheric conditions (80% N(2), 10% CO(2,) 10% H(2)) for 24 hours, 48 hours, and 72 hours under anaerobic conditions in a CO(2) incubator. The diameters of the zones of bacterial and fungal growth inhibition around the wells containing the test substances are then recorded after the period of incubation. The inhibitory zone determined in millimeter by measuring scale the shortest distance between the outer margin of the well and initial microbial as well as fungal growth. The experiments were performed 20 times and the mean and standard deviations of the inhibitory zones were calculated. RESULT: Platelet rich fibrin is mixed with nanosilver particles showed higher antimicrobial efficacy than platelet rich plasma with nanosilver and simple platelet rich plasma and platelet rich fibrin are equivalent when it is placed against the anaerobic bacteria E.faecalis and yeast like fungi Candida albicans, respectively. CONCLUSION: Groups presented with antimicrobial efficacy in this order- Group IV > Group II > Group III > Group I. |
format | Online Article Text |
id | pubmed-10485451 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-104854512023-09-09 To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver Zafar, Adil Tripathi, Vartika Khan, Mariyam Manglam, Keshav K. Rastogi, Preeti Almotreb, Abdullah M. J Pharm Bioallied Sci Original Article AIM: To evaluate the antimicrobial activity of PRP and PRF with and without nanosilver. MATERIALS AND METHODS: The materials were tested in powdered form is nanosilver. The nanosilver particles was mixed to form with PRP and PRF so as to placed in a wells followed the groups are experimental groups; Group I: PRP + nanosilver particles, Group II: PRF + nanosilver and control group: PRP and PRF and normal saline. Silver nanoparticles was tested at concentrations of 50 μ gram per mL. The powder was prepared for each group with identical amount of the powder (milligram/mg) and then mixed with 1 milliliter liquid. The plates are then incubated at 37°C under appropriate atmospheric conditions (80% N(2), 10% CO(2,) 10% H(2)) for 24 hours, 48 hours, and 72 hours under anaerobic conditions in a CO(2) incubator. The diameters of the zones of bacterial and fungal growth inhibition around the wells containing the test substances are then recorded after the period of incubation. The inhibitory zone determined in millimeter by measuring scale the shortest distance between the outer margin of the well and initial microbial as well as fungal growth. The experiments were performed 20 times and the mean and standard deviations of the inhibitory zones were calculated. RESULT: Platelet rich fibrin is mixed with nanosilver particles showed higher antimicrobial efficacy than platelet rich plasma with nanosilver and simple platelet rich plasma and platelet rich fibrin are equivalent when it is placed against the anaerobic bacteria E.faecalis and yeast like fungi Candida albicans, respectively. CONCLUSION: Groups presented with antimicrobial efficacy in this order- Group IV > Group II > Group III > Group I. Wolters Kluwer - Medknow 2023-07 2023-07-11 /pmc/articles/PMC10485451/ /pubmed/37694058 http://dx.doi.org/10.4103/jpbs.jpbs_72_23 Text en Copyright: © 2023 Journal of Pharmacy and Bioallied Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Zafar, Adil Tripathi, Vartika Khan, Mariyam Manglam, Keshav K. Rastogi, Preeti Almotreb, Abdullah M. To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver |
title | To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver |
title_full | To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver |
title_fullStr | To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver |
title_full_unstemmed | To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver |
title_short | To Evaluate the Antimicrobial Activity of PRP and PRF with and without Nanosilver |
title_sort | to evaluate the antimicrobial activity of prp and prf with and without nanosilver |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485451/ https://www.ncbi.nlm.nih.gov/pubmed/37694058 http://dx.doi.org/10.4103/jpbs.jpbs_72_23 |
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