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Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI
One of the most common forms of the mammalian central nervous system (CNS) injuries is spinal cord injury (SCI), and any lesion to the CNS can result in a lifelong functional impairment since CNS axons cannot regenerate. The relative axon regenerating genes following spinal SCI were examined using t...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Wolters Kluwer - Medknow
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485452/ https://www.ncbi.nlm.nih.gov/pubmed/37693980 http://dx.doi.org/10.4103/jpbs.jpbs_199_23 |
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author | Alhajlah, Sharif |
author_facet | Alhajlah, Sharif |
author_sort | Alhajlah, Sharif |
collection | PubMed |
description | One of the most common forms of the mammalian central nervous system (CNS) injuries is spinal cord injury (SCI), and any lesion to the CNS can result in a lifelong functional impairment since CNS axons cannot regenerate. The relative axon regenerating genes following spinal SCI were examined using the regenerative SN, pSN + DC, and non-regenerating DC lesion models. By using qRT-PCR, we discovered that fibroblast growth factor receptor-5 (FGFR5) was 4.2-fold more highly expressed in non-regeneration lesions compared to intact control and regenerating animals. Furthermore, in cultured dorsal root ganglion neurons (DRGN), short interfering RNA (siRNA)-mediated knockdown of FGFR5 had no effect on DRGN neurite outgrowth, indicating that the gene’s suppression has no effect on axon regeneration and may play other roles in the CNS besides axon regeneration. |
format | Online Article Text |
id | pubmed-10485452 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-104854522023-09-09 Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI Alhajlah, Sharif J Pharm Bioallied Sci Original Article One of the most common forms of the mammalian central nervous system (CNS) injuries is spinal cord injury (SCI), and any lesion to the CNS can result in a lifelong functional impairment since CNS axons cannot regenerate. The relative axon regenerating genes following spinal SCI were examined using the regenerative SN, pSN + DC, and non-regenerating DC lesion models. By using qRT-PCR, we discovered that fibroblast growth factor receptor-5 (FGFR5) was 4.2-fold more highly expressed in non-regeneration lesions compared to intact control and regenerating animals. Furthermore, in cultured dorsal root ganglion neurons (DRGN), short interfering RNA (siRNA)-mediated knockdown of FGFR5 had no effect on DRGN neurite outgrowth, indicating that the gene’s suppression has no effect on axon regeneration and may play other roles in the CNS besides axon regeneration. Wolters Kluwer - Medknow 2023-07 2023-07-11 /pmc/articles/PMC10485452/ /pubmed/37693980 http://dx.doi.org/10.4103/jpbs.jpbs_199_23 Text en Copyright: © 2023 Journal of Pharmacy and Bioallied Sciences https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Alhajlah, Sharif Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI |
title | Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI |
title_full | Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI |
title_fullStr | Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI |
title_full_unstemmed | Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI |
title_short | Suppression of Fibroblast Growth Factor Receptor-5 (FGFR5) has no Impact on Axon Regeneration after SCI |
title_sort | suppression of fibroblast growth factor receptor-5 (fgfr5) has no impact on axon regeneration after sci |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485452/ https://www.ncbi.nlm.nih.gov/pubmed/37693980 http://dx.doi.org/10.4103/jpbs.jpbs_199_23 |
work_keys_str_mv | AT alhajlahsharif suppressionoffibroblastgrowthfactorreceptor5fgfr5hasnoimpactonaxonregenerationaftersci |