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Dynamics of miRNA biogenesis and nuclear transport
MicroRNAs (miRNAs) are short noncoding RNA sequences ~22 nucleotides in length that play an important role in gene regulation-transcription and translation. The processing of these miRNAs takes place in both the nucleus and the cytoplasm while the final maturation occurs in the cytoplasm. Some matur...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
De Gruyter
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485738/ https://www.ncbi.nlm.nih.gov/pubmed/34348448 http://dx.doi.org/10.1515/jib-2016-305 |
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author | Kotipalli, Aneesh Gutti, Ravikumar Mitra, Chanchal K. |
author_facet | Kotipalli, Aneesh Gutti, Ravikumar Mitra, Chanchal K. |
author_sort | Kotipalli, Aneesh |
collection | PubMed |
description | MicroRNAs (miRNAs) are short noncoding RNA sequences ~22 nucleotides in length that play an important role in gene regulation-transcription and translation. The processing of these miRNAs takes place in both the nucleus and the cytoplasm while the final maturation occurs in the cytoplasm. Some mature miRNAs with nuclear localisation signals (NLS) are transported back to the nucleus and some remain in the cytoplasm. The functional roles of these miRNAs are seen in both the nucleus and the cytoplasm. In the nucleus, miRNAs regulate gene expression by binding to the targeted promoter sequences and affect either the transcriptional gene silencing (TGS) or transcriptional gene activation (TGA). In the cytoplasm, targeted mRNAs are translationally repressed or cleaved based on the complementarity between the two sequences at the seed region of miRNA and mRNA. The selective transport of mature miRNAs to the nucleus follows the classical nuclear import mechanism. The classical nuclear import mechanism is a highly regulated process, involving exportins and importins. The nuclear pore complex (NPC) regulates all these transport events like a gate keeper. The half-life of miRNAs is rather low, so within a short time miRNAs perform their function. Temporal studies of miRNA biogenesis are, therefore, useful. We have carried out simulation studies for important miRNA biogenesis steps and also classical nuclear import mechanism using ordinary differential equation (ODE) solver in the Octave software. |
format | Online Article Text |
id | pubmed-10485738 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | De Gruyter |
record_format | MEDLINE/PubMed |
spelling | pubmed-104857382023-09-09 Dynamics of miRNA biogenesis and nuclear transport Kotipalli, Aneesh Gutti, Ravikumar Mitra, Chanchal K. J Integr Bioinform Article MicroRNAs (miRNAs) are short noncoding RNA sequences ~22 nucleotides in length that play an important role in gene regulation-transcription and translation. The processing of these miRNAs takes place in both the nucleus and the cytoplasm while the final maturation occurs in the cytoplasm. Some mature miRNAs with nuclear localisation signals (NLS) are transported back to the nucleus and some remain in the cytoplasm. The functional roles of these miRNAs are seen in both the nucleus and the cytoplasm. In the nucleus, miRNAs regulate gene expression by binding to the targeted promoter sequences and affect either the transcriptional gene silencing (TGS) or transcriptional gene activation (TGA). In the cytoplasm, targeted mRNAs are translationally repressed or cleaved based on the complementarity between the two sequences at the seed region of miRNA and mRNA. The selective transport of mature miRNAs to the nucleus follows the classical nuclear import mechanism. The classical nuclear import mechanism is a highly regulated process, involving exportins and importins. The nuclear pore complex (NPC) regulates all these transport events like a gate keeper. The half-life of miRNAs is rather low, so within a short time miRNAs perform their function. Temporal studies of miRNA biogenesis are, therefore, useful. We have carried out simulation studies for important miRNA biogenesis steps and also classical nuclear import mechanism using ordinary differential equation (ODE) solver in the Octave software. De Gruyter 2017-04-20 /pmc/articles/PMC10485738/ /pubmed/34348448 http://dx.doi.org/10.1515/jib-2016-305 Text en © 2016 The Author(s). Published by Journal of Integrative Bioinformatics. https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License. |
spellingShingle | Article Kotipalli, Aneesh Gutti, Ravikumar Mitra, Chanchal K. Dynamics of miRNA biogenesis and nuclear transport |
title | Dynamics of miRNA biogenesis and nuclear transport |
title_full | Dynamics of miRNA biogenesis and nuclear transport |
title_fullStr | Dynamics of miRNA biogenesis and nuclear transport |
title_full_unstemmed | Dynamics of miRNA biogenesis and nuclear transport |
title_short | Dynamics of miRNA biogenesis and nuclear transport |
title_sort | dynamics of mirna biogenesis and nuclear transport |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485738/ https://www.ncbi.nlm.nih.gov/pubmed/34348448 http://dx.doi.org/10.1515/jib-2016-305 |
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