An improved CRISPRi system in Pichia pastoris

CRISPR interference (CRISPRi) has been developed and widely used for gene repression in various hosts. Here we report an improved CRISPRi system in Pichia pastoris by fusing dCas9 with endogenous transcriptional repressor domains. The CRISPRi system shows strong repression of eGFP, with the highest...

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Detalles Bibliográficos
Autores principales: Qiao, Shujing, Bai, Fan, Cai, Peng, Zhou, Yongjin J., Yao, Lun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485788/
https://www.ncbi.nlm.nih.gov/pubmed/37692202
http://dx.doi.org/10.1016/j.synbio.2023.06.008

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10485788/
https://www.ncbi.nlm.nih.gov/pubmed/37692202
http://dx.doi.org/10.1016/j.synbio.2023.06.008

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