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Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease

BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the main reason for cirrhosis and hepatocellular carcinoma. As a starting point for NAFLD, the treatment of nonalcoholic fatty liver (NAFL) is receiving increasing attention. Mice fed a high‐fat diet (HFD) and hereditary leptin deficiency (ob/o...

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Autores principales: Ding, Yiming, Dai, Xulei, Bao, Miaoye, Xing, Yuanming, Liu, Junhui, Zhao, Sihai, Liu, Enqi, Yuan, Zuyi, Bai, Liang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486336/
https://www.ncbi.nlm.nih.gov/pubmed/37565549
http://dx.doi.org/10.1002/ame2.12338
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author Ding, Yiming
Dai, Xulei
Bao, Miaoye
Xing, Yuanming
Liu, Junhui
Zhao, Sihai
Liu, Enqi
Yuan, Zuyi
Bai, Liang
author_facet Ding, Yiming
Dai, Xulei
Bao, Miaoye
Xing, Yuanming
Liu, Junhui
Zhao, Sihai
Liu, Enqi
Yuan, Zuyi
Bai, Liang
author_sort Ding, Yiming
collection PubMed
description BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the main reason for cirrhosis and hepatocellular carcinoma. As a starting point for NAFLD, the treatment of nonalcoholic fatty liver (NAFL) is receiving increasing attention. Mice fed a high‐fat diet (HFD) and hereditary leptin deficiency (ob/ob) mice are important NAFL animal models. However, the comparison of these mouse models with human NAFL is still unclear. METHODS: In this study, HFD‐fed mice and ob/ob mice were used as NAFL animal models. Liver histopathological characteristics were compared, and liver transcriptome from both mouse models was performed using RNA sequencing (RNA‐seq). RNA‐seq data obtained from the livers of NAFL patients was downloaded from the GEO database. Global gene expression profiles in the livers were further analyzed using functional enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. RESULTS: Our results showed that the biochemical parameters of both mouse models and human NAFL were similar. Compared with HFD‐fed mice, ob/ob mice were more similar in histologic appearance to NAFL patients. The liver transcriptome characteristics partly overlapped in mice and humans. Furthermore, in the NAFL pathway, most genes showed similar trends in mice and humans, thus demonstrating that both types of mice can be used as models for basic research on NAFL, considering the differences. CONCLUSION: Our findings show that HFD‐fed mice and ob/ob mice can mimic human NAFL partly in pathophysiological process. The comparative analysis of liver transcriptome profile in mouse models and human NAFL presented here provides insights into the molecular characteristics across these NAFL models.
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spelling pubmed-104863362023-09-09 Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease Ding, Yiming Dai, Xulei Bao, Miaoye Xing, Yuanming Liu, Junhui Zhao, Sihai Liu, Enqi Yuan, Zuyi Bai, Liang Animal Model Exp Med Themed Section: Multi‐omics Data Analysis of Experimental Animals BACKGROUND: Nonalcoholic fatty liver disease (NAFLD) is the main reason for cirrhosis and hepatocellular carcinoma. As a starting point for NAFLD, the treatment of nonalcoholic fatty liver (NAFL) is receiving increasing attention. Mice fed a high‐fat diet (HFD) and hereditary leptin deficiency (ob/ob) mice are important NAFL animal models. However, the comparison of these mouse models with human NAFL is still unclear. METHODS: In this study, HFD‐fed mice and ob/ob mice were used as NAFL animal models. Liver histopathological characteristics were compared, and liver transcriptome from both mouse models was performed using RNA sequencing (RNA‐seq). RNA‐seq data obtained from the livers of NAFL patients was downloaded from the GEO database. Global gene expression profiles in the livers were further analyzed using functional enrichment analysis and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway. RESULTS: Our results showed that the biochemical parameters of both mouse models and human NAFL were similar. Compared with HFD‐fed mice, ob/ob mice were more similar in histologic appearance to NAFL patients. The liver transcriptome characteristics partly overlapped in mice and humans. Furthermore, in the NAFL pathway, most genes showed similar trends in mice and humans, thus demonstrating that both types of mice can be used as models for basic research on NAFL, considering the differences. CONCLUSION: Our findings show that HFD‐fed mice and ob/ob mice can mimic human NAFL partly in pathophysiological process. The comparative analysis of liver transcriptome profile in mouse models and human NAFL presented here provides insights into the molecular characteristics across these NAFL models. John Wiley and Sons Inc. 2023-08-11 /pmc/articles/PMC10486336/ /pubmed/37565549 http://dx.doi.org/10.1002/ame2.12338 Text en © 2023 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.
spellingShingle Themed Section: Multi‐omics Data Analysis of Experimental Animals
Ding, Yiming
Dai, Xulei
Bao, Miaoye
Xing, Yuanming
Liu, Junhui
Zhao, Sihai
Liu, Enqi
Yuan, Zuyi
Bai, Liang
Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
title Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
title_full Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
title_fullStr Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
title_full_unstemmed Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
title_short Hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
title_sort hepatic transcriptome signatures in mice and humans with nonalcoholic fatty liver disease
topic Themed Section: Multi‐omics Data Analysis of Experimental Animals
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486336/
https://www.ncbi.nlm.nih.gov/pubmed/37565549
http://dx.doi.org/10.1002/ame2.12338
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