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Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans

Ramulus mori has been widely used in traditional Chinese medicine because of its physiological activities, including antibacterial, anti-inflammatory, and antioxidant activities. Antimicrobial properties of Ramulus mori extract have been well described. However, no information is available regarding...

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Autores principales: Li, Erna, Li, Shipei, Wang, Siyuan, Li, Qian, Pang, Daorui, Yang, Qiong, Zhu, Qiaoling, Zou, Yuxiao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486356/
https://www.ncbi.nlm.nih.gov/pubmed/37685114
http://dx.doi.org/10.3390/foods12173182
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author Li, Erna
Li, Shipei
Wang, Siyuan
Li, Qian
Pang, Daorui
Yang, Qiong
Zhu, Qiaoling
Zou, Yuxiao
author_facet Li, Erna
Li, Shipei
Wang, Siyuan
Li, Qian
Pang, Daorui
Yang, Qiong
Zhu, Qiaoling
Zou, Yuxiao
author_sort Li, Erna
collection PubMed
description Ramulus mori has been widely used in traditional Chinese medicine because of its physiological activities, including antibacterial, anti-inflammatory, and antioxidant activities. Antimicrobial properties of Ramulus mori extract have been well described. However, no information is available regarding on Ramulus mori oligosaccharides (RMOS). The aim of this study was to investigate the effects of RMOS on the growth and virulence properties of the cariogenic bacterium Streptococcus mutans. The effects of RMOS on the biofilm structure and virulence gene expression of S. mutans were also evaluated, and the results were compared with the effects of commercial prebiotic galactooligosaccharides. RMOS were found to have an antibacterial effect against S. mutans, resulting in significant reductions in acid production, lactate dehydrogenase activity, adhesion, insoluble extracellular polysaccharide production, glucosyltransferase activity, and biofilm formation in a dose-dependent manner. Moreover, the biofilm structure was visibly damaged. A quantitative real-time PCR assay revealed downregulation of virulence gene-regulated acid production, polysaccharide production, adhesion, biofilm formation, and quorum sensing. These findings suggest that RMOS may be a promising natural product for the prevention of dental caries.
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spelling pubmed-104863562023-09-09 Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans Li, Erna Li, Shipei Wang, Siyuan Li, Qian Pang, Daorui Yang, Qiong Zhu, Qiaoling Zou, Yuxiao Foods Article Ramulus mori has been widely used in traditional Chinese medicine because of its physiological activities, including antibacterial, anti-inflammatory, and antioxidant activities. Antimicrobial properties of Ramulus mori extract have been well described. However, no information is available regarding on Ramulus mori oligosaccharides (RMOS). The aim of this study was to investigate the effects of RMOS on the growth and virulence properties of the cariogenic bacterium Streptococcus mutans. The effects of RMOS on the biofilm structure and virulence gene expression of S. mutans were also evaluated, and the results were compared with the effects of commercial prebiotic galactooligosaccharides. RMOS were found to have an antibacterial effect against S. mutans, resulting in significant reductions in acid production, lactate dehydrogenase activity, adhesion, insoluble extracellular polysaccharide production, glucosyltransferase activity, and biofilm formation in a dose-dependent manner. Moreover, the biofilm structure was visibly damaged. A quantitative real-time PCR assay revealed downregulation of virulence gene-regulated acid production, polysaccharide production, adhesion, biofilm formation, and quorum sensing. These findings suggest that RMOS may be a promising natural product for the prevention of dental caries. MDPI 2023-08-24 /pmc/articles/PMC10486356/ /pubmed/37685114 http://dx.doi.org/10.3390/foods12173182 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Erna
Li, Shipei
Wang, Siyuan
Li, Qian
Pang, Daorui
Yang, Qiong
Zhu, Qiaoling
Zou, Yuxiao
Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans
title Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans
title_full Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans
title_fullStr Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans
title_full_unstemmed Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans
title_short Antibacterial Effects of Ramulus mori Oligosaccharides against Streptococcus mutans
title_sort antibacterial effects of ramulus mori oligosaccharides against streptococcus mutans
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486356/
https://www.ncbi.nlm.nih.gov/pubmed/37685114
http://dx.doi.org/10.3390/foods12173182
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