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Species of Botryosphaeriaceae associated with citrus branch diseases in China

Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of Botryosphaeriaceae are known as important fungal pathog...

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Autores principales: Xiao, X.E., Wang, W., Crous, P.W., Wang, H.K., Jiao, C., Huang, F., Pu, Z.X., Zhu, Z.R., Li, H.Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nationaal Herbarium Nederland & Westerdijk Fungal Biodiversity Institute 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486630/
https://www.ncbi.nlm.nih.gov/pubmed/37693792
http://dx.doi.org/10.3767/persoonia.2021.47.03
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author Xiao, X.E.
Wang, W.
Crous, P.W.
Wang, H.K.
Jiao, C.
Huang, F.
Pu, Z.X.
Zhu, Z.R.
Li, H.Y.
author_facet Xiao, X.E.
Wang, W.
Crous, P.W.
Wang, H.K.
Jiao, C.
Huang, F.
Pu, Z.X.
Zhu, Z.R.
Li, H.Y.
author_sort Xiao, X.E.
collection PubMed
description Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of Botryosphaeriaceae are known as important fungal pathogens causing branch diseases on citrus in the USA and Europe. To determine the diversity of Botryosphaeriaceae species associated with citrus branch diseases in China, surveys were conducted in the major citrus-producing areas from 2017 to 2020. Diseased tissues were collected from twigs, branches and trunks with a range of symptoms including cankers, cracking, dieback and gummosis. Based on morphological characteristics and phylogenetic comparison of the DNA sequences of the internal transcribed spacer region (ITS), the translation elongation factor 1-alpha gene (tef1), the β-tubulin gene (tub2) and the DNA-directed RNA polymerase II second largest subunit (rpb2), 111 isolates from nine provinces were identified as 18 species of Botryosphaeriaceae, including Botryosphaeria dothidea, B. fabicerciana, Diplodia seriata, Dothiorella alpina, Do. plurivora, Lasiodiplodia citricola, L. iraniensis, L. microconidia, L. pseudotheobromae, L. theobromae, Neodeightonia subglobosa, Neofusicoccum parvum, and six previously undescribed species, namely Do. citrimurcotticola, L. guilinensis, L. huangyanensis, L. linhaiensis, L. ponkanicola and Sphaeropsis linhaiensis spp. nov. Botryosphaeria dothidea (28.8 %) was the most abundant species, followed by L. pseudotheobromae (23.4 %), which was the most widely distributed species on citrus, occurring in six of the nine provinces sampled. Pathogenicity tests indicated that all 18 species of Botryosphaeriaceae obtained from diseased citrus tissues in this study were pathogenic to the tested Citrus reticulata shoots in vitro, while not all species are pathogenic to the tested Cocktail grapefruit (C. paradisi × C. reticulata) shoots in vivo. In addition, Lasiodiplodia was the most aggressive genus both in vitro and in vivo. This is the first study to identify Botryosphaeriaceae species related to citrus branch diseases in China and the results provide a theoretical basis for the implementation of prevention and control measures. Citation: Xiao XE, Wang W, Crous PW, et al. 2021. Species of Botryosphaeriaceae associated with citrus branch diseases in China. Persoonia 47: 106–135. https://doi.org/10.3767/persoonia.2021.47.03.
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spelling pubmed-104866302023-09-08 Species of Botryosphaeriaceae associated with citrus branch diseases in China Xiao, X.E. Wang, W. Crous, P.W. Wang, H.K. Jiao, C. Huang, F. Pu, Z.X. Zhu, Z.R. Li, H.Y. Persoonia Research Article Citrus is an important and widely cultivated fruit crop in South China. Although the species of fungal diseases of leaves and fruits have been extensively studied, the causal organisms of branch diseases remain poorly known in China. Species of Botryosphaeriaceae are known as important fungal pathogens causing branch diseases on citrus in the USA and Europe. To determine the diversity of Botryosphaeriaceae species associated with citrus branch diseases in China, surveys were conducted in the major citrus-producing areas from 2017 to 2020. Diseased tissues were collected from twigs, branches and trunks with a range of symptoms including cankers, cracking, dieback and gummosis. Based on morphological characteristics and phylogenetic comparison of the DNA sequences of the internal transcribed spacer region (ITS), the translation elongation factor 1-alpha gene (tef1), the β-tubulin gene (tub2) and the DNA-directed RNA polymerase II second largest subunit (rpb2), 111 isolates from nine provinces were identified as 18 species of Botryosphaeriaceae, including Botryosphaeria dothidea, B. fabicerciana, Diplodia seriata, Dothiorella alpina, Do. plurivora, Lasiodiplodia citricola, L. iraniensis, L. microconidia, L. pseudotheobromae, L. theobromae, Neodeightonia subglobosa, Neofusicoccum parvum, and six previously undescribed species, namely Do. citrimurcotticola, L. guilinensis, L. huangyanensis, L. linhaiensis, L. ponkanicola and Sphaeropsis linhaiensis spp. nov. Botryosphaeria dothidea (28.8 %) was the most abundant species, followed by L. pseudotheobromae (23.4 %), which was the most widely distributed species on citrus, occurring in six of the nine provinces sampled. Pathogenicity tests indicated that all 18 species of Botryosphaeriaceae obtained from diseased citrus tissues in this study were pathogenic to the tested Citrus reticulata shoots in vitro, while not all species are pathogenic to the tested Cocktail grapefruit (C. paradisi × C. reticulata) shoots in vivo. In addition, Lasiodiplodia was the most aggressive genus both in vitro and in vivo. This is the first study to identify Botryosphaeriaceae species related to citrus branch diseases in China and the results provide a theoretical basis for the implementation of prevention and control measures. Citation: Xiao XE, Wang W, Crous PW, et al. 2021. Species of Botryosphaeriaceae associated with citrus branch diseases in China. Persoonia 47: 106–135. https://doi.org/10.3767/persoonia.2021.47.03. Nationaal Herbarium Nederland & Westerdijk Fungal Biodiversity Institute 2021-01-13 2021-12 /pmc/articles/PMC10486630/ /pubmed/37693792 http://dx.doi.org/10.3767/persoonia.2021.47.03 Text en © 2021 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute https://creativecommons.org/licenses/by-nc-nd/3.0/You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution:You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial:You may not use this work for commercial purposes. No derivative works:You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode (https://creativecommons.org/licenses/by-nc-nd/3.0/) . Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights.
spellingShingle Research Article
Xiao, X.E.
Wang, W.
Crous, P.W.
Wang, H.K.
Jiao, C.
Huang, F.
Pu, Z.X.
Zhu, Z.R.
Li, H.Y.
Species of Botryosphaeriaceae associated with citrus branch diseases in China
title Species of Botryosphaeriaceae associated with citrus branch diseases in China
title_full Species of Botryosphaeriaceae associated with citrus branch diseases in China
title_fullStr Species of Botryosphaeriaceae associated with citrus branch diseases in China
title_full_unstemmed Species of Botryosphaeriaceae associated with citrus branch diseases in China
title_short Species of Botryosphaeriaceae associated with citrus branch diseases in China
title_sort species of botryosphaeriaceae associated with citrus branch diseases in china
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486630/
https://www.ncbi.nlm.nih.gov/pubmed/37693792
http://dx.doi.org/10.3767/persoonia.2021.47.03
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