Cargando…
The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses
Fungi are rich in complexes of cryptic species that need a combination of different approaches to be delimited, including genomic information. Beauveria (Cordycipitaceae, Hypocreales) is a well-known genus of entomopathogenic fungi, used as a biocontrol agent. In this study we present a polyphasic t...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nationaal Herbarium Nederland & Centraallbureau voor Schimmelcultures
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486633/ https://www.ncbi.nlm.nih.gov/pubmed/37693793 http://dx.doi.org/10.3767/persoonia.2021.47.04 |
_version_ | 1785103052814942208 |
---|---|
author | Kobmoo, N. Arnamnart, N. Pootakham, W. Sonthirod, C. Khonsanit, A. Kuephadungphan, W. Suntivich, R. Mosunova, O.V. Giraud, T. Luangsa-ard, J.J. |
author_facet | Kobmoo, N. Arnamnart, N. Pootakham, W. Sonthirod, C. Khonsanit, A. Kuephadungphan, W. Suntivich, R. Mosunova, O.V. Giraud, T. Luangsa-ard, J.J. |
author_sort | Kobmoo, N. |
collection | PubMed |
description | Fungi are rich in complexes of cryptic species that need a combination of different approaches to be delimited, including genomic information. Beauveria (Cordycipitaceae, Hypocreales) is a well-known genus of entomopathogenic fungi, used as a biocontrol agent. In this study we present a polyphasic taxonomy regarding two widely distributed complexes of Beauveria: B. asiatica and B. bassiana s.lat. Some of the genetic groups as previously detected within both taxa were either confirmed or fused using population genomics. High levels of divergence were found between two clades in B. asiatica and among three clades in B. bassiana, supporting their subdivision as distinct species. Morphological examination focusing on the width and the length of phialides and conidia showed no difference among the clades within B. bassiana while conidial length was significantly different among clades within B. asiatica. The secondary metabolite profiles obtained by liquid chromatography-mass spectrometry (LC-MS) allowed a distinction between B. asiatica and B. bassiana, but not between the clades therein. Based on these genomic, morphological, chemical data, we proposed a clade of B. asiatica as a new species, named B. thailandica, and two clades of B. bassiana to respectively represent B. namnaoensis and B. neobassiana spp. nov. Such closely related but divergent species with different host ranges have potential to elucidate the evolution of host specificity, with potential biocontrol application. Citation: Kobmoo N, Arnamnart N, Pootakham W, et al. 2021. The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses. Persoonia 47: 136–150. https://doi.org/10.3767/persoonia.2021.47.04. |
format | Online Article Text |
id | pubmed-10486633 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Nationaal Herbarium Nederland & Centraallbureau voor Schimmelcultures |
record_format | MEDLINE/PubMed |
spelling | pubmed-104866332023-09-08 The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses Kobmoo, N. Arnamnart, N. Pootakham, W. Sonthirod, C. Khonsanit, A. Kuephadungphan, W. Suntivich, R. Mosunova, O.V. Giraud, T. Luangsa-ard, J.J. Persoonia Research Article Fungi are rich in complexes of cryptic species that need a combination of different approaches to be delimited, including genomic information. Beauveria (Cordycipitaceae, Hypocreales) is a well-known genus of entomopathogenic fungi, used as a biocontrol agent. In this study we present a polyphasic taxonomy regarding two widely distributed complexes of Beauveria: B. asiatica and B. bassiana s.lat. Some of the genetic groups as previously detected within both taxa were either confirmed or fused using population genomics. High levels of divergence were found between two clades in B. asiatica and among three clades in B. bassiana, supporting their subdivision as distinct species. Morphological examination focusing on the width and the length of phialides and conidia showed no difference among the clades within B. bassiana while conidial length was significantly different among clades within B. asiatica. The secondary metabolite profiles obtained by liquid chromatography-mass spectrometry (LC-MS) allowed a distinction between B. asiatica and B. bassiana, but not between the clades therein. Based on these genomic, morphological, chemical data, we proposed a clade of B. asiatica as a new species, named B. thailandica, and two clades of B. bassiana to respectively represent B. namnaoensis and B. neobassiana spp. nov. Such closely related but divergent species with different host ranges have potential to elucidate the evolution of host specificity, with potential biocontrol application. Citation: Kobmoo N, Arnamnart N, Pootakham W, et al. 2021. The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses. Persoonia 47: 136–150. https://doi.org/10.3767/persoonia.2021.47.04. Nationaal Herbarium Nederland & Centraallbureau voor Schimmelcultures 2021-10-01 2021-12 /pmc/articles/PMC10486633/ /pubmed/37693793 http://dx.doi.org/10.3767/persoonia.2021.47.04 Text en © 2021 Naturalis Biodiversity Center & Westerdijk Fungal Biodiversity Institute https://creativecommons.org/licenses/by-nc-nd/3.0/You are free to share - to copy, distribute and transmit the work, under the following conditions: Attribution: You must attribute the work in the manner specified by the author or licensor (but not in any way that suggests that they endorse you or your use of the work). Non-commercial: You may not use this work for commercial purposes. No derivative works: You may not alter, transform, or build upon this work. For any reuse or distribution, you must make clear to others the license terms of this work, which can be found at http://creativecommons.org/licenses/by-nc-nd/3.0/legalcode (https://creativecommons.org/licenses/by-nc-nd/3.0/) . Any of the above conditions can be waived if you get permission from the copyright holder. Nothing in this license impairs or restricts the author’s moral rights. |
spellingShingle | Research Article Kobmoo, N. Arnamnart, N. Pootakham, W. Sonthirod, C. Khonsanit, A. Kuephadungphan, W. Suntivich, R. Mosunova, O.V. Giraud, T. Luangsa-ard, J.J. The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
title | The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
title_full | The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
title_fullStr | The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
title_full_unstemmed | The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
title_short | The integrative taxonomy of Beauveria asiatica and B. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
title_sort | integrative taxonomy of beauveria asiatica and b. bassiana species complexes with whole-genome sequencing, morphometric and chemical analyses |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486633/ https://www.ncbi.nlm.nih.gov/pubmed/37693793 http://dx.doi.org/10.3767/persoonia.2021.47.04 |
work_keys_str_mv | AT kobmoon theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT arnamnartn theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT pootakhamw theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT sonthirodc theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT khonsanita theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT kuephadungphanw theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT suntivichr theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT mosunovaov theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT giraudt theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT luangsaardjj theintegrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT kobmoon integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT arnamnartn integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT pootakhamw integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT sonthirodc integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT khonsanita integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT kuephadungphanw integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT suntivichr integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT mosunovaov integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT giraudt integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses AT luangsaardjj integrativetaxonomyofbeauveriaasiaticaandbbassianaspeciescomplexeswithwholegenomesequencingmorphometricandchemicalanalyses |