Cargando…

Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue

Psoriasis is a frequent and incurable skin disease whose pathogenesis is still not fully understood. It is characterized by immune disturbances leading to hyperproliferation and improper differentiation of keratinocytes. Gasdermin E (GSDME) is a protein from the gasdermin family involved in the proc...

Descripción completa

Detalles Bibliográficos
Autores principales: Nowowiejska, Julia, Baran, Anna, Pryczynicz, Anna, Hermanowicz, Justyna Magdalena, Sieklucka, Beata, Pawlak, Dariusz, Flisiak, Iwona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486754/
https://www.ncbi.nlm.nih.gov/pubmed/37681881
http://dx.doi.org/10.3390/cells12172149
_version_ 1785103081991569408
author Nowowiejska, Julia
Baran, Anna
Pryczynicz, Anna
Hermanowicz, Justyna Magdalena
Sieklucka, Beata
Pawlak, Dariusz
Flisiak, Iwona
author_facet Nowowiejska, Julia
Baran, Anna
Pryczynicz, Anna
Hermanowicz, Justyna Magdalena
Sieklucka, Beata
Pawlak, Dariusz
Flisiak, Iwona
author_sort Nowowiejska, Julia
collection PubMed
description Psoriasis is a frequent and incurable skin disease whose pathogenesis is still not fully understood. It is characterized by immune disturbances leading to hyperproliferation and improper differentiation of keratinocytes. Gasdermin E (GSDME) is a protein from the gasdermin family involved in the processes of inflammation and cell death based on apoptosis, necroptosis and pyroptosis. It has never been studied in psoriatics’ sera or urine before. Our study enrolled 60 patients with psoriasis and 30 volunteers without dermatoses as controls. Serum and urinary GSDME concentrations were examined by ELISA and tissue expression of GSDME by immunohistochemistry. Serum GSDME concentration was significantly higher in patients than controls (p < 0.05). There were no differences in urinary GSDME concentrations between patients and controls. GSDME expression was significantly higher in the psoriatic plaque than non-lesional patients’ skin and compared to controls (both p < 0.001). There was no correlation between serum GSDME or its lesional expression and psoriasis severity, age or disease duration. GSDME serum concentration was significantly negatively correlated with BMI, triglycerides and glucose concentrations. The obtained results suggest the engagement of GSDME in psoriasis pathogenesis. It could potentially become a new non-invasive psoriasis marker. Considering its pro-apoptotic influence, GSDME could be compensatively elevated to direct cells towards apoptosis, whereas under other circumstances, it may lead to pyroptosis and sustain inflammation. GSDME may exert a protective influence on the metabolic complications in psoriasis which requires further studies.
format Online
Article
Text
id pubmed-10486754
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104867542023-09-09 Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue Nowowiejska, Julia Baran, Anna Pryczynicz, Anna Hermanowicz, Justyna Magdalena Sieklucka, Beata Pawlak, Dariusz Flisiak, Iwona Cells Article Psoriasis is a frequent and incurable skin disease whose pathogenesis is still not fully understood. It is characterized by immune disturbances leading to hyperproliferation and improper differentiation of keratinocytes. Gasdermin E (GSDME) is a protein from the gasdermin family involved in the processes of inflammation and cell death based on apoptosis, necroptosis and pyroptosis. It has never been studied in psoriatics’ sera or urine before. Our study enrolled 60 patients with psoriasis and 30 volunteers without dermatoses as controls. Serum and urinary GSDME concentrations were examined by ELISA and tissue expression of GSDME by immunohistochemistry. Serum GSDME concentration was significantly higher in patients than controls (p < 0.05). There were no differences in urinary GSDME concentrations between patients and controls. GSDME expression was significantly higher in the psoriatic plaque than non-lesional patients’ skin and compared to controls (both p < 0.001). There was no correlation between serum GSDME or its lesional expression and psoriasis severity, age or disease duration. GSDME serum concentration was significantly negatively correlated with BMI, triglycerides and glucose concentrations. The obtained results suggest the engagement of GSDME in psoriasis pathogenesis. It could potentially become a new non-invasive psoriasis marker. Considering its pro-apoptotic influence, GSDME could be compensatively elevated to direct cells towards apoptosis, whereas under other circumstances, it may lead to pyroptosis and sustain inflammation. GSDME may exert a protective influence on the metabolic complications in psoriasis which requires further studies. MDPI 2023-08-26 /pmc/articles/PMC10486754/ /pubmed/37681881 http://dx.doi.org/10.3390/cells12172149 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Nowowiejska, Julia
Baran, Anna
Pryczynicz, Anna
Hermanowicz, Justyna Magdalena
Sieklucka, Beata
Pawlak, Dariusz
Flisiak, Iwona
Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
title Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
title_full Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
title_fullStr Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
title_full_unstemmed Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
title_short Gasdermin E (GSDME)—A New Potential Marker of Psoriasis and Its Metabolic Complications: The First Combined Study on Human Serum, Urine and Tissue
title_sort gasdermin e (gsdme)—a new potential marker of psoriasis and its metabolic complications: the first combined study on human serum, urine and tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10486754/
https://www.ncbi.nlm.nih.gov/pubmed/37681881
http://dx.doi.org/10.3390/cells12172149
work_keys_str_mv AT nowowiejskajulia gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue
AT barananna gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue
AT pryczyniczanna gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue
AT hermanowiczjustynamagdalena gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue
AT siekluckabeata gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue
AT pawlakdariusz gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue
AT flisiakiwona gasderminegsdmeanewpotentialmarkerofpsoriasisanditsmetaboliccomplicationsthefirstcombinedstudyonhumanserumurineandtissue