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The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections

Globally, over 3.5 billion people are infected with intestinal parasites each year, resulting in over 200,000 deaths. Three of the most common protozoan pathogens that affect the gastrointestinal tract of humans are Cryptosporidium spp., Giardia intestinalis, and Entamoeba histolytica. Other protozo...

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Autores principales: Aghazadeh, Mahdis, Jones, Meghan, Perera, Suneth, Nair, Jiny, Tan, Litty, Clark, Brett, Curtis, Angela, Jones, Jackson, Ellem, Justin, Olma, Tom, Stark, Damien, Melki, John, Coulston, Neralie, Baker, Rohan, Millar, Douglas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487386/
https://www.ncbi.nlm.nih.gov/pubmed/37686192
http://dx.doi.org/10.3390/ijms241713387
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author Aghazadeh, Mahdis
Jones, Meghan
Perera, Suneth
Nair, Jiny
Tan, Litty
Clark, Brett
Curtis, Angela
Jones, Jackson
Ellem, Justin
Olma, Tom
Stark, Damien
Melki, John
Coulston, Neralie
Baker, Rohan
Millar, Douglas
author_facet Aghazadeh, Mahdis
Jones, Meghan
Perera, Suneth
Nair, Jiny
Tan, Litty
Clark, Brett
Curtis, Angela
Jones, Jackson
Ellem, Justin
Olma, Tom
Stark, Damien
Melki, John
Coulston, Neralie
Baker, Rohan
Millar, Douglas
author_sort Aghazadeh, Mahdis
collection PubMed
description Globally, over 3.5 billion people are infected with intestinal parasites each year, resulting in over 200,000 deaths. Three of the most common protozoan pathogens that affect the gastrointestinal tract of humans are Cryptosporidium spp., Giardia intestinalis, and Entamoeba histolytica. Other protozoan agents that have been implicated in gastroenteritis in humans include Cyclospora cayetanensis, Dientamoeba fragilis, Blastocystis hominis, and the microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis. Genetic Signatures previously developed a 3base™ multiplexed Real-Time PCR (mRT-PCR) enteric protozoan kit (EP001) for the detection of Giardia intestinalis/lamblia/duodenalis, Cryptosporidium spp., E. histolytica, D. fragilis, and B. hominis. We now describe improvements to this kit to produce a more comprehensive assay, including C. cayetanensis, E. bieneusi, and E. intestinalis, termed EP005. The clinical performance of EP005 was assessed using a set of 380 clinical samples against a commercially available PCR test and other in-house nucleic acid amplification tests where commercial tests were not available. All methods provided at least 90% agreement. EP005 had no cross-reactivity against 82 organisms commonly found in the gut. The EP005 method streamlines the detection of gastrointestinal parasites and addresses the many challenges of traditional microscopic detection, resulting in cost savings and significant improvements in patient care.
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spelling pubmed-104873862023-09-09 The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections Aghazadeh, Mahdis Jones, Meghan Perera, Suneth Nair, Jiny Tan, Litty Clark, Brett Curtis, Angela Jones, Jackson Ellem, Justin Olma, Tom Stark, Damien Melki, John Coulston, Neralie Baker, Rohan Millar, Douglas Int J Mol Sci Article Globally, over 3.5 billion people are infected with intestinal parasites each year, resulting in over 200,000 deaths. Three of the most common protozoan pathogens that affect the gastrointestinal tract of humans are Cryptosporidium spp., Giardia intestinalis, and Entamoeba histolytica. Other protozoan agents that have been implicated in gastroenteritis in humans include Cyclospora cayetanensis, Dientamoeba fragilis, Blastocystis hominis, and the microsporidia Enterocytozoon bieneusi and Encephalitozoon intestinalis. Genetic Signatures previously developed a 3base™ multiplexed Real-Time PCR (mRT-PCR) enteric protozoan kit (EP001) for the detection of Giardia intestinalis/lamblia/duodenalis, Cryptosporidium spp., E. histolytica, D. fragilis, and B. hominis. We now describe improvements to this kit to produce a more comprehensive assay, including C. cayetanensis, E. bieneusi, and E. intestinalis, termed EP005. The clinical performance of EP005 was assessed using a set of 380 clinical samples against a commercially available PCR test and other in-house nucleic acid amplification tests where commercial tests were not available. All methods provided at least 90% agreement. EP005 had no cross-reactivity against 82 organisms commonly found in the gut. The EP005 method streamlines the detection of gastrointestinal parasites and addresses the many challenges of traditional microscopic detection, resulting in cost savings and significant improvements in patient care. MDPI 2023-08-29 /pmc/articles/PMC10487386/ /pubmed/37686192 http://dx.doi.org/10.3390/ijms241713387 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aghazadeh, Mahdis
Jones, Meghan
Perera, Suneth
Nair, Jiny
Tan, Litty
Clark, Brett
Curtis, Angela
Jones, Jackson
Ellem, Justin
Olma, Tom
Stark, Damien
Melki, John
Coulston, Neralie
Baker, Rohan
Millar, Douglas
The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
title The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
title_full The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
title_fullStr The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
title_full_unstemmed The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
title_short The Application of 3base™ Technology to Diagnose Eight of the Most Clinically Important Gastrointestinal Protozoan Infections
title_sort application of 3base™ technology to diagnose eight of the most clinically important gastrointestinal protozoan infections
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487386/
https://www.ncbi.nlm.nih.gov/pubmed/37686192
http://dx.doi.org/10.3390/ijms241713387
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