Cargando…

Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain

Insulin-like androgenic gland hormone (IAG) is a key regulator of male sexual differentiation in crustaceans that plays important roles in secondary sexual characteristics and testicular development. As a hormone, IAG interacts with its membrane receptor to initiate downstream signal pathways to exe...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, An, Hao, Shuang, Liu, Fang, Huang, Huiyang, Ye, Haihui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487528/
https://www.ncbi.nlm.nih.gov/pubmed/37686442
http://dx.doi.org/10.3390/ijms241713639
_version_ 1785103265066647552
author Liu, An
Hao, Shuang
Liu, Fang
Huang, Huiyang
Ye, Haihui
author_facet Liu, An
Hao, Shuang
Liu, Fang
Huang, Huiyang
Ye, Haihui
author_sort Liu, An
collection PubMed
description Insulin-like androgenic gland hormone (IAG) is a key regulator of male sexual differentiation in crustaceans that plays important roles in secondary sexual characteristics and testicular development. As a hormone, IAG interacts with its membrane receptor to initiate downstream signal pathways to exert its biological functions. In this study, we isolated a full-length cDNA of an insulin-like receptor (Sp-IR) from the mud crab Scylla paramamosain. Sequence analysis revealed that this receptor consists of a Fu domain, two L domains, three FN-III domains, a transmembrane domain, and a tyrosine kinase domain, classifying it as a member of the tyrosine kinase insulin-like receptors family. Our results also suggested that Sp-IR was highly expressed in the testis and AG in males. Its expression in the testis peaked in stage I but significantly decreased in stages II and III (p < 0.01). Next, both short- and long-term RNA interference (RNAi) experiments were performed on males in stage I to explore Sp-IR function in mud crabs. The results showed that Sp-vasa and Sp-Dsx expression levels in the testis were significantly down-regulated after the specific knockdown of Sp-IR by RNAi. Additionally, the long-term knockdown of Sp-IR led to a considerable decrease in the volume of seminiferous tubules, accompanied by large vacuoles and a reduced production of secondary spermatocytes and spermatids. In conclusion, our results indicated that Sp-IR is involved in testicular development and plays a crucial role in transitioning from primary to secondary spermatocytes. This study provided a molecular basis for the subsequent analysis of the mechanism on male sexual differentiation in Brachyuran crabs.
format Online
Article
Text
id pubmed-10487528
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104875282023-09-09 Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain Liu, An Hao, Shuang Liu, Fang Huang, Huiyang Ye, Haihui Int J Mol Sci Article Insulin-like androgenic gland hormone (IAG) is a key regulator of male sexual differentiation in crustaceans that plays important roles in secondary sexual characteristics and testicular development. As a hormone, IAG interacts with its membrane receptor to initiate downstream signal pathways to exert its biological functions. In this study, we isolated a full-length cDNA of an insulin-like receptor (Sp-IR) from the mud crab Scylla paramamosain. Sequence analysis revealed that this receptor consists of a Fu domain, two L domains, three FN-III domains, a transmembrane domain, and a tyrosine kinase domain, classifying it as a member of the tyrosine kinase insulin-like receptors family. Our results also suggested that Sp-IR was highly expressed in the testis and AG in males. Its expression in the testis peaked in stage I but significantly decreased in stages II and III (p < 0.01). Next, both short- and long-term RNA interference (RNAi) experiments were performed on males in stage I to explore Sp-IR function in mud crabs. The results showed that Sp-vasa and Sp-Dsx expression levels in the testis were significantly down-regulated after the specific knockdown of Sp-IR by RNAi. Additionally, the long-term knockdown of Sp-IR led to a considerable decrease in the volume of seminiferous tubules, accompanied by large vacuoles and a reduced production of secondary spermatocytes and spermatids. In conclusion, our results indicated that Sp-IR is involved in testicular development and plays a crucial role in transitioning from primary to secondary spermatocytes. This study provided a molecular basis for the subsequent analysis of the mechanism on male sexual differentiation in Brachyuran crabs. MDPI 2023-09-04 /pmc/articles/PMC10487528/ /pubmed/37686442 http://dx.doi.org/10.3390/ijms241713639 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, An
Hao, Shuang
Liu, Fang
Huang, Huiyang
Ye, Haihui
Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain
title Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain
title_full Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain
title_fullStr Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain
title_full_unstemmed Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain
title_short Isolation of an Insulin-Like Receptor Involved in the Testicular Development of the Mud Crab Scylla paramamosain
title_sort isolation of an insulin-like receptor involved in the testicular development of the mud crab scylla paramamosain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487528/
https://www.ncbi.nlm.nih.gov/pubmed/37686442
http://dx.doi.org/10.3390/ijms241713639
work_keys_str_mv AT liuan isolationofaninsulinlikereceptorinvolvedinthetesticulardevelopmentofthemudcrabscyllaparamamosain
AT haoshuang isolationofaninsulinlikereceptorinvolvedinthetesticulardevelopmentofthemudcrabscyllaparamamosain
AT liufang isolationofaninsulinlikereceptorinvolvedinthetesticulardevelopmentofthemudcrabscyllaparamamosain
AT huanghuiyang isolationofaninsulinlikereceptorinvolvedinthetesticulardevelopmentofthemudcrabscyllaparamamosain
AT yehaihui isolationofaninsulinlikereceptorinvolvedinthetesticulardevelopmentofthemudcrabscyllaparamamosain