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Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation

During ischemia and reperfusion injury (IRI), mitochondria may release mitochondrial DNA (mtDNA). mtDNA can serve as a propagator of further injury but in specific settings has anti-inflammatory capacities as well. Therefore, the aim of this study was to study the perioperative dynamics of plasma mt...

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Autores principales: Kroneisl, Marie, Spraakman, Nora A., Koomen, Jeroen V., Hijazi, Zeinab, Hoogstra-Berends, Femke H., Leuvenink, Henri G. D., Struys, Michel M. R. F., Henning, Rob H., Nieuwenhuijs-Moeke, Gertrude J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487554/
https://www.ncbi.nlm.nih.gov/pubmed/37686384
http://dx.doi.org/10.3390/ijms241713579
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author Kroneisl, Marie
Spraakman, Nora A.
Koomen, Jeroen V.
Hijazi, Zeinab
Hoogstra-Berends, Femke H.
Leuvenink, Henri G. D.
Struys, Michel M. R. F.
Henning, Rob H.
Nieuwenhuijs-Moeke, Gertrude J.
author_facet Kroneisl, Marie
Spraakman, Nora A.
Koomen, Jeroen V.
Hijazi, Zeinab
Hoogstra-Berends, Femke H.
Leuvenink, Henri G. D.
Struys, Michel M. R. F.
Henning, Rob H.
Nieuwenhuijs-Moeke, Gertrude J.
author_sort Kroneisl, Marie
collection PubMed
description During ischemia and reperfusion injury (IRI), mitochondria may release mitochondrial DNA (mtDNA). mtDNA can serve as a propagator of further injury but in specific settings has anti-inflammatory capacities as well. Therefore, the aim of this study was to study the perioperative dynamics of plasma mtDNA during living donor kidney transplantation (LDKT) and its potential as a marker of graft outcome. Fifty-six donor–recipient couples from the Volatile Anesthetic Protection of Renal Transplants-1 (VAPOR-1) trial were included. Systemic venous, systemic arterial, and renal venous samples were taken at multiple timepoints during and after LDKT. Levels of mtDNA genes changed over time and between vascular compartments. Several donor, recipient, and transplantation-related variables significantly explained the course of mtDNA genes over time. mtDNA genes predicted 1-month and 24-month estimated glomerular filtration rate (eGFR) and acute rejection episodes in the two-year follow-up period. To conclude, mtDNA is released in plasma during the process of LDKT, either from the kidney or from the whole body in response to transplantation. While circulating mtDNA levels positively and negatively predict post-transplantation outcomes, the exact mechanisms and difference between mtDNA genes are not yet understood and need further exploration.
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spelling pubmed-104875542023-09-09 Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation Kroneisl, Marie Spraakman, Nora A. Koomen, Jeroen V. Hijazi, Zeinab Hoogstra-Berends, Femke H. Leuvenink, Henri G. D. Struys, Michel M. R. F. Henning, Rob H. Nieuwenhuijs-Moeke, Gertrude J. Int J Mol Sci Article During ischemia and reperfusion injury (IRI), mitochondria may release mitochondrial DNA (mtDNA). mtDNA can serve as a propagator of further injury but in specific settings has anti-inflammatory capacities as well. Therefore, the aim of this study was to study the perioperative dynamics of plasma mtDNA during living donor kidney transplantation (LDKT) and its potential as a marker of graft outcome. Fifty-six donor–recipient couples from the Volatile Anesthetic Protection of Renal Transplants-1 (VAPOR-1) trial were included. Systemic venous, systemic arterial, and renal venous samples were taken at multiple timepoints during and after LDKT. Levels of mtDNA genes changed over time and between vascular compartments. Several donor, recipient, and transplantation-related variables significantly explained the course of mtDNA genes over time. mtDNA genes predicted 1-month and 24-month estimated glomerular filtration rate (eGFR) and acute rejection episodes in the two-year follow-up period. To conclude, mtDNA is released in plasma during the process of LDKT, either from the kidney or from the whole body in response to transplantation. While circulating mtDNA levels positively and negatively predict post-transplantation outcomes, the exact mechanisms and difference between mtDNA genes are not yet understood and need further exploration. MDPI 2023-09-01 /pmc/articles/PMC10487554/ /pubmed/37686384 http://dx.doi.org/10.3390/ijms241713579 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kroneisl, Marie
Spraakman, Nora A.
Koomen, Jeroen V.
Hijazi, Zeinab
Hoogstra-Berends, Femke H.
Leuvenink, Henri G. D.
Struys, Michel M. R. F.
Henning, Rob H.
Nieuwenhuijs-Moeke, Gertrude J.
Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
title Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
title_full Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
title_fullStr Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
title_full_unstemmed Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
title_short Peri-Operative Kinetics of Plasma Mitochondrial DNA Levels during Living Donor Kidney Transplantation
title_sort peri-operative kinetics of plasma mitochondrial dna levels during living donor kidney transplantation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487554/
https://www.ncbi.nlm.nih.gov/pubmed/37686384
http://dx.doi.org/10.3390/ijms241713579
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