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Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation

The yield and quality of peanut (Arachis hypogaea L.), an oil crop planted worldwide, are often limited by drought stress (DS) and nitrogen (N) deficiency. To investigate the molecular mechanism by which peanut counteracts DS and N deficiency, we conducted comprehensive transcriptomic and metabolomi...

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Autores principales: Ding, Hong, Dai, Liangxiang, Guo, Qing, Chen, Xiaoshu, Zhang, Guanchu, Feng, Hao, Qin, Feifei, Gao, Huayuan, Xu, Yang, Zhang, Zhimeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487698/
https://www.ncbi.nlm.nih.gov/pubmed/37686113
http://dx.doi.org/10.3390/ijms241713308
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author Ding, Hong
Dai, Liangxiang
Guo, Qing
Chen, Xiaoshu
Zhang, Guanchu
Feng, Hao
Qin, Feifei
Gao, Huayuan
Xu, Yang
Zhang, Zhimeng
author_facet Ding, Hong
Dai, Liangxiang
Guo, Qing
Chen, Xiaoshu
Zhang, Guanchu
Feng, Hao
Qin, Feifei
Gao, Huayuan
Xu, Yang
Zhang, Zhimeng
author_sort Ding, Hong
collection PubMed
description The yield and quality of peanut (Arachis hypogaea L.), an oil crop planted worldwide, are often limited by drought stress (DS) and nitrogen (N) deficiency. To investigate the molecular mechanism by which peanut counteracts DS and N deficiency, we conducted comprehensive transcriptomic and metabolomic analyses of peanut leaves. Herein, 829 known differentially accumulated metabolites, 324 differentially expressed transcription factors, and 5294 differentially expressed genes (DEGs) were identified under different water and N conditions. The transcriptome analysis demonstrated that drought-related DEGs were predominantly expressed in “glycolysis/gluconeogenesis” and “glycerolipid metabolism”, while N-deficiency-related DEGs were mainly expressed in starch and sucrose metabolism, as well as in the biosynthesis of amino acid pathways. The biosynthesis, transport, and catabolism of secondary metabolites accounted for a large proportion of the 1317 DEGs present in water and N co–limitation. Metabolomic analysis showed that the metabolic accumulation of these pathways was significantly dependent on the stress conditions. Additionally, the roles of metabolites and genes in these pathways, such as the biosynthesis of amino acids and phenylpropanoid biosynthesis under different stress conditions, were discussed. The results demonstrated that different genes, metabolic pathways, and metabolites were related to DS and N deficiency. Thus, this study elucidates the metabolic pathways and functional genes that can be used for the improvement of peanut resistance to abiotic stress.
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spelling pubmed-104876982023-09-09 Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation Ding, Hong Dai, Liangxiang Guo, Qing Chen, Xiaoshu Zhang, Guanchu Feng, Hao Qin, Feifei Gao, Huayuan Xu, Yang Zhang, Zhimeng Int J Mol Sci Article The yield and quality of peanut (Arachis hypogaea L.), an oil crop planted worldwide, are often limited by drought stress (DS) and nitrogen (N) deficiency. To investigate the molecular mechanism by which peanut counteracts DS and N deficiency, we conducted comprehensive transcriptomic and metabolomic analyses of peanut leaves. Herein, 829 known differentially accumulated metabolites, 324 differentially expressed transcription factors, and 5294 differentially expressed genes (DEGs) were identified under different water and N conditions. The transcriptome analysis demonstrated that drought-related DEGs were predominantly expressed in “glycolysis/gluconeogenesis” and “glycerolipid metabolism”, while N-deficiency-related DEGs were mainly expressed in starch and sucrose metabolism, as well as in the biosynthesis of amino acid pathways. The biosynthesis, transport, and catabolism of secondary metabolites accounted for a large proportion of the 1317 DEGs present in water and N co–limitation. Metabolomic analysis showed that the metabolic accumulation of these pathways was significantly dependent on the stress conditions. Additionally, the roles of metabolites and genes in these pathways, such as the biosynthesis of amino acids and phenylpropanoid biosynthesis under different stress conditions, were discussed. The results demonstrated that different genes, metabolic pathways, and metabolites were related to DS and N deficiency. Thus, this study elucidates the metabolic pathways and functional genes that can be used for the improvement of peanut resistance to abiotic stress. MDPI 2023-08-27 /pmc/articles/PMC10487698/ /pubmed/37686113 http://dx.doi.org/10.3390/ijms241713308 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ding, Hong
Dai, Liangxiang
Guo, Qing
Chen, Xiaoshu
Zhang, Guanchu
Feng, Hao
Qin, Feifei
Gao, Huayuan
Xu, Yang
Zhang, Zhimeng
Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation
title Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation
title_full Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation
title_fullStr Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation
title_full_unstemmed Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation
title_short Comprehensive Transcriptome and Metabolome Analyses Reveal Primary Molecular Regulation Pathways Involved in Peanut under Water and Nitrogen Co-Limitation
title_sort comprehensive transcriptome and metabolome analyses reveal primary molecular regulation pathways involved in peanut under water and nitrogen co-limitation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487698/
https://www.ncbi.nlm.nih.gov/pubmed/37686113
http://dx.doi.org/10.3390/ijms241713308
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