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Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response
Lysin-Motif receptor-like kinase (LysM-RLK) proteins are widely distributed in plants and serve a critical role in defending against pathogens and establishing symbiotic relationships. However, there is a lack of comprehensive identification and analysis of LysM-RLK family members in the soybean gen...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487828/ https://www.ncbi.nlm.nih.gov/pubmed/37686427 http://dx.doi.org/10.3390/ijms241713621 |
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author | Yao, Kaijie Wang, Yongliang Li, Xia Ji, Hongtao |
author_facet | Yao, Kaijie Wang, Yongliang Li, Xia Ji, Hongtao |
author_sort | Yao, Kaijie |
collection | PubMed |
description | Lysin-Motif receptor-like kinase (LysM-RLK) proteins are widely distributed in plants and serve a critical role in defending against pathogens and establishing symbiotic relationships. However, there is a lack of comprehensive identification and analysis of LysM-RLK family members in the soybean genome. In this study, we discovered and named 27 LysM-RLK genes in soybean. The majority of LysM-RLKs were highly conserved in Arabidopsis and soybean, while certain members of subclades III, VI, and VII are unique to soybean. The promoters of these LysM-RLKs contain specific cis-elements associated with plant development and responses to environmental factors. Notably, all LysM-RLK gene promoters feature nodule specificity elements, while 51.86% of them also possess NBS sites (NIN/NLP binding site). The expression profiles revealed that genes from subclade V in soybean roots were regulated by both rhizobia and nitrogen treatment. The expression levels of subclade V genes were then validated by real-time quantitative PCR, and it was observed that the level of GmLYK4a and GmLYK4c in roots was inhibited by rhizobia but induced via varying concentrations of nitrate. Consequently, our findings provide a comprehensive understanding of the soybean LysM-RLK gene family and emphasize the role of subclade V in coupling soybean symbiotic nitrogen fixation and nitrogen response. |
format | Online Article Text |
id | pubmed-10487828 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104878282023-09-09 Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response Yao, Kaijie Wang, Yongliang Li, Xia Ji, Hongtao Int J Mol Sci Article Lysin-Motif receptor-like kinase (LysM-RLK) proteins are widely distributed in plants and serve a critical role in defending against pathogens and establishing symbiotic relationships. However, there is a lack of comprehensive identification and analysis of LysM-RLK family members in the soybean genome. In this study, we discovered and named 27 LysM-RLK genes in soybean. The majority of LysM-RLKs were highly conserved in Arabidopsis and soybean, while certain members of subclades III, VI, and VII are unique to soybean. The promoters of these LysM-RLKs contain specific cis-elements associated with plant development and responses to environmental factors. Notably, all LysM-RLK gene promoters feature nodule specificity elements, while 51.86% of them also possess NBS sites (NIN/NLP binding site). The expression profiles revealed that genes from subclade V in soybean roots were regulated by both rhizobia and nitrogen treatment. The expression levels of subclade V genes were then validated by real-time quantitative PCR, and it was observed that the level of GmLYK4a and GmLYK4c in roots was inhibited by rhizobia but induced via varying concentrations of nitrate. Consequently, our findings provide a comprehensive understanding of the soybean LysM-RLK gene family and emphasize the role of subclade V in coupling soybean symbiotic nitrogen fixation and nitrogen response. MDPI 2023-09-03 /pmc/articles/PMC10487828/ /pubmed/37686427 http://dx.doi.org/10.3390/ijms241713621 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yao, Kaijie Wang, Yongliang Li, Xia Ji, Hongtao Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response |
title | Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response |
title_full | Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response |
title_fullStr | Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response |
title_full_unstemmed | Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response |
title_short | Genome-Wide Identification of the Soybean LysM-RLK Family Genes and Its Nitrogen Response |
title_sort | genome-wide identification of the soybean lysm-rlk family genes and its nitrogen response |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487828/ https://www.ncbi.nlm.nih.gov/pubmed/37686427 http://dx.doi.org/10.3390/ijms241713621 |
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