Effects of sgRNAs, Promoters, and Explants on the Gene Editing Efficiency of the CRISPR/Cas9 System in Chinese Kale

The CRISPR/Cas9 system is extensively used for plant gene editing. This study developed an efficient CRISPR/Cas9 system for Chinese kale using multiple sgRNAs and two promoters to create various CRISPR/Cas9 vectors. These vectors targeted BoaZDS and BoaCRTISO in Chinese kale protoplasts and cotyledo...

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Detalles Bibliográficos
Autores principales: Huang, Wenli, Zheng, Aihong, Huang, Huanhuan, Chen, Zhifeng, Ma, Jie, Li, Xiangxiang, Liang, Qiannan, Li, Ling, Liu, Ruobin, Huang, Zhi, Qin, Yaoguo, Tang, Yi, Li, Huanxiu, Zhang, Fen, Wang, Qiaomei, Sun, Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487834/
https://www.ncbi.nlm.nih.gov/pubmed/37686051
http://dx.doi.org/10.3390/ijms241713241

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