Non-Covalent Interactions between dUTP C5-Substituents and DNA Polymerase Decrease PCR Efficiency

The approach based on molecular modeling was developed to study dNTP derivatives characterized by new polymerase-specific properties. For this purpose, the relative efficiency of PCR amplification with modified dUTPs was studied using Taq, Tth, Pfu, Vent, Deep Vent, Vent (exo-), and Deep Vent (exo-)...

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Detalles Bibliográficos
Autores principales: Zasedateleva, Olga A., Surzhikov, Sergey A., Kuznetsova, Viktoriya E., Shershov, Valeriy E., Barsky, Victor E., Zasedatelev, Alexander S., Chudinov, Alexander V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487964/
https://www.ncbi.nlm.nih.gov/pubmed/37686447
http://dx.doi.org/10.3390/ijms241713643

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487964/
https://www.ncbi.nlm.nih.gov/pubmed/37686447
http://dx.doi.org/10.3390/ijms241713643

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