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Non-Covalent Interactions between dUTP C5-Substituents and DNA Polymerase Decrease PCR Efficiency

The approach based on molecular modeling was developed to study dNTP derivatives characterized by new polymerase-specific properties. For this purpose, the relative efficiency of PCR amplification with modified dUTPs was studied using Taq, Tth, Pfu, Vent, Deep Vent, Vent (exo-), and Deep Vent (exo-)...

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Detalles Bibliográficos
Autores principales:	Zasedateleva, Olga A., Surzhikov, Sergey A., Kuznetsova, Viktoriya E., Shershov, Valeriy E., Barsky, Victor E., Zasedatelev, Alexander S., Chudinov, Alexander V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487964/ https://www.ncbi.nlm.nih.gov/pubmed/37686447 http://dx.doi.org/10.3390/ijms241713643

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