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The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids

The objective of this study was to determine if the aberrant expression of select genes could form the basis for the racial disparity in fibroid characteristics. The next-generation RNA sequencing results were analyzed as fold change [leiomyomas/paired myometrium, also known as differential expressi...

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Autores principales: Chuang, Tsai-Der, Ton, Nhu, Rysling, Shawn, Quintanilla, Derek, Boos, Drake, Gao, Jianjun, McSwiggin, Hayden, Yan, Wei, Khorram, Omid
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487975/
https://www.ncbi.nlm.nih.gov/pubmed/37686244
http://dx.doi.org/10.3390/ijms241713441
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author Chuang, Tsai-Der
Ton, Nhu
Rysling, Shawn
Quintanilla, Derek
Boos, Drake
Gao, Jianjun
McSwiggin, Hayden
Yan, Wei
Khorram, Omid
author_facet Chuang, Tsai-Der
Ton, Nhu
Rysling, Shawn
Quintanilla, Derek
Boos, Drake
Gao, Jianjun
McSwiggin, Hayden
Yan, Wei
Khorram, Omid
author_sort Chuang, Tsai-Der
collection PubMed
description The objective of this study was to determine if the aberrant expression of select genes could form the basis for the racial disparity in fibroid characteristics. The next-generation RNA sequencing results were analyzed as fold change [leiomyomas/paired myometrium, also known as differential expression (DF)], comparing specimens from White (n = 7) and Black (n = 12) patients. The analysis indicated that 95 genes were minimally changed in tumors from White (DF ≈ 1) but were significantly altered by more than 1.5-fold (up or down) in Black patients. Twenty-one novel genes were selected for confirmation in 69 paired fibroids by qRT-PCR. Among these 21, coding of transcripts for the differential expression of FRAT2, SOX4, TNFRSF19, ACP7, GRIP1, IRS4, PLEKHG4B, PGR, COL24A1, KRT17, MMP17, SLN, CCDC177, FUT2, MYO5B, MYOG, ZNF703, CDC25A, and CDCA7 was significantly higher, while the expression of DAB2 and CAV2 was significantly lower in tumors from Black or Hispanic patients compared with tumors from White patients. Western blot analysis revealed a greater differential expression of PGR-A and total progesterone (PGR-A and PGR-B) in tumors from Black compared with tumors from White patients. Collectively, we identified a set of genes uniquely expressed in a race/ethnicity-dependent manner, which could form the underlying mechanisms for the racial disparity in fibroids and their associated symptoms.
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spelling pubmed-104879752023-09-09 The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids Chuang, Tsai-Der Ton, Nhu Rysling, Shawn Quintanilla, Derek Boos, Drake Gao, Jianjun McSwiggin, Hayden Yan, Wei Khorram, Omid Int J Mol Sci Article The objective of this study was to determine if the aberrant expression of select genes could form the basis for the racial disparity in fibroid characteristics. The next-generation RNA sequencing results were analyzed as fold change [leiomyomas/paired myometrium, also known as differential expression (DF)], comparing specimens from White (n = 7) and Black (n = 12) patients. The analysis indicated that 95 genes were minimally changed in tumors from White (DF ≈ 1) but were significantly altered by more than 1.5-fold (up or down) in Black patients. Twenty-one novel genes were selected for confirmation in 69 paired fibroids by qRT-PCR. Among these 21, coding of transcripts for the differential expression of FRAT2, SOX4, TNFRSF19, ACP7, GRIP1, IRS4, PLEKHG4B, PGR, COL24A1, KRT17, MMP17, SLN, CCDC177, FUT2, MYO5B, MYOG, ZNF703, CDC25A, and CDCA7 was significantly higher, while the expression of DAB2 and CAV2 was significantly lower in tumors from Black or Hispanic patients compared with tumors from White patients. Western blot analysis revealed a greater differential expression of PGR-A and total progesterone (PGR-A and PGR-B) in tumors from Black compared with tumors from White patients. Collectively, we identified a set of genes uniquely expressed in a race/ethnicity-dependent manner, which could form the underlying mechanisms for the racial disparity in fibroids and their associated symptoms. MDPI 2023-08-30 /pmc/articles/PMC10487975/ /pubmed/37686244 http://dx.doi.org/10.3390/ijms241713441 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chuang, Tsai-Der
Ton, Nhu
Rysling, Shawn
Quintanilla, Derek
Boos, Drake
Gao, Jianjun
McSwiggin, Hayden
Yan, Wei
Khorram, Omid
The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids
title The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids
title_full The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids
title_fullStr The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids
title_full_unstemmed The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids
title_short The Influence of Race/Ethnicity on the Transcriptomic Landscape of Uterine Fibroids
title_sort influence of race/ethnicity on the transcriptomic landscape of uterine fibroids
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10487975/
https://www.ncbi.nlm.nih.gov/pubmed/37686244
http://dx.doi.org/10.3390/ijms241713441
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