Cargando…
Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis
PEAR proteins are a type of plant-specific DNA binding with one finger (Dof) transcription factors that play a key role in the regulation of plant growth, especially during phloem cell growth and seed germination in Arabidopsis. However, the identification, characteristics and function of PEAR prote...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488302/ https://www.ncbi.nlm.nih.gov/pubmed/37685908 http://dx.doi.org/10.3390/ijms241713101 |
_version_ | 1785103446549987328 |
---|---|
author | Li, Ping Wang, Jing Jiang, Derui Yu, Anmin Sun, Rui Liu, Aizhong |
author_facet | Li, Ping Wang, Jing Jiang, Derui Yu, Anmin Sun, Rui Liu, Aizhong |
author_sort | Li, Ping |
collection | PubMed |
description | PEAR proteins are a type of plant-specific DNA binding with one finger (Dof) transcription factors that play a key role in the regulation of plant growth, especially during phloem cell growth and seed germination in Arabidopsis. However, the identification, characteristics and function of PEAR proteins, particularly in woody plants, need to be further studied. In the present study, 43 candidate PEAR proteins harboring the conserved Zf-Dof domain were obtained in Populus yunnanensis. Based on phylogenetic and structural analysis, 10 representative PEAR candidates were selected, belonging to different phylogenetic groups. The functions of PEAR proteins in the stress response, signal transduction, and growth regulation of stem cambium and roots undergoing vigorous cell division in Arabidopsis were revealed based on their expression patterns as characterized by qRT-PCR analysis, in accordance with the results of cis-element analysis. In vitro experiments showed that the interaction of transcription factor (E2F) and cyclin indirectly reflects the growth regulation function of PEAR through light signaling and cell-cycle regulation. Therefore, our results provide new insight into the identity of PEAR proteins and their function in stress resistance and vigorous cell division regulation of tissues in P. yunnanensis, which may serve as a basis for further investigation of the functions and characteristics of PEAR proteins in other plants. |
format | Online Article Text |
id | pubmed-10488302 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104883022023-09-09 Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis Li, Ping Wang, Jing Jiang, Derui Yu, Anmin Sun, Rui Liu, Aizhong Int J Mol Sci Article PEAR proteins are a type of plant-specific DNA binding with one finger (Dof) transcription factors that play a key role in the regulation of plant growth, especially during phloem cell growth and seed germination in Arabidopsis. However, the identification, characteristics and function of PEAR proteins, particularly in woody plants, need to be further studied. In the present study, 43 candidate PEAR proteins harboring the conserved Zf-Dof domain were obtained in Populus yunnanensis. Based on phylogenetic and structural analysis, 10 representative PEAR candidates were selected, belonging to different phylogenetic groups. The functions of PEAR proteins in the stress response, signal transduction, and growth regulation of stem cambium and roots undergoing vigorous cell division in Arabidopsis were revealed based on their expression patterns as characterized by qRT-PCR analysis, in accordance with the results of cis-element analysis. In vitro experiments showed that the interaction of transcription factor (E2F) and cyclin indirectly reflects the growth regulation function of PEAR through light signaling and cell-cycle regulation. Therefore, our results provide new insight into the identity of PEAR proteins and their function in stress resistance and vigorous cell division regulation of tissues in P. yunnanensis, which may serve as a basis for further investigation of the functions and characteristics of PEAR proteins in other plants. MDPI 2023-08-23 /pmc/articles/PMC10488302/ /pubmed/37685908 http://dx.doi.org/10.3390/ijms241713101 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Ping Wang, Jing Jiang, Derui Yu, Anmin Sun, Rui Liu, Aizhong Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis |
title | Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis |
title_full | Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis |
title_fullStr | Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis |
title_full_unstemmed | Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis |
title_short | Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis |
title_sort | function and characteristic analysis of candidate pear proteins in populus yunnanensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488302/ https://www.ncbi.nlm.nih.gov/pubmed/37685908 http://dx.doi.org/10.3390/ijms241713101 |
work_keys_str_mv | AT liping functionandcharacteristicanalysisofcandidatepearproteinsinpopulusyunnanensis AT wangjing functionandcharacteristicanalysisofcandidatepearproteinsinpopulusyunnanensis AT jiangderui functionandcharacteristicanalysisofcandidatepearproteinsinpopulusyunnanensis AT yuanmin functionandcharacteristicanalysisofcandidatepearproteinsinpopulusyunnanensis AT sunrui functionandcharacteristicanalysisofcandidatepearproteinsinpopulusyunnanensis AT liuaizhong functionandcharacteristicanalysisofcandidatepearproteinsinpopulusyunnanensis |