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New Advances in the Exploration of Esterases with PET and Fluorescent Probes
Esterases are hydrolases that catalyze the hydrolysis of esters into the corresponding acids and alcohols. The development of fluorescent probes for detecting esterases is of great importance due to their wide spectrum of biological and industrial applications. These probes can provide a rapid and s...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488407/ https://www.ncbi.nlm.nih.gov/pubmed/37687094 http://dx.doi.org/10.3390/molecules28176265 |
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author | Gil-Rivas, Alba de Pascual-Teresa, Beatriz Ortín, Irene Ramos, Ana |
author_facet | Gil-Rivas, Alba de Pascual-Teresa, Beatriz Ortín, Irene Ramos, Ana |
author_sort | Gil-Rivas, Alba |
collection | PubMed |
description | Esterases are hydrolases that catalyze the hydrolysis of esters into the corresponding acids and alcohols. The development of fluorescent probes for detecting esterases is of great importance due to their wide spectrum of biological and industrial applications. These probes can provide a rapid and sensitive method for detecting the presence and activity of esterases in various samples, including biological fluids, food products, and environmental samples. Fluorescent probes can also be used for monitoring the effects of drugs and environmental toxins on esterase activity, as well as to study the functions and mechanisms of these enzymes in several biological systems. Additionally, fluorescent probes can be designed to selectively target specific types of esterases, such as those found in pathogenic bacteria or cancer cells. In this review, we summarize the recent fluorescent probes described for the visualization of cell viability and some applications for in vivo imaging. On the other hand, positron emission tomography (PET) is a nuclear-based molecular imaging modality of great value for studying the activity of enzymes in vivo. We provide some examples of PET probes for imaging acetylcholinesterases and butyrylcholinesterases in the brain, which are valuable tools for diagnosing dementia and monitoring the effects of anticholinergic drugs on the central nervous system. |
format | Online Article Text |
id | pubmed-10488407 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104884072023-09-09 New Advances in the Exploration of Esterases with PET and Fluorescent Probes Gil-Rivas, Alba de Pascual-Teresa, Beatriz Ortín, Irene Ramos, Ana Molecules Review Esterases are hydrolases that catalyze the hydrolysis of esters into the corresponding acids and alcohols. The development of fluorescent probes for detecting esterases is of great importance due to their wide spectrum of biological and industrial applications. These probes can provide a rapid and sensitive method for detecting the presence and activity of esterases in various samples, including biological fluids, food products, and environmental samples. Fluorescent probes can also be used for monitoring the effects of drugs and environmental toxins on esterase activity, as well as to study the functions and mechanisms of these enzymes in several biological systems. Additionally, fluorescent probes can be designed to selectively target specific types of esterases, such as those found in pathogenic bacteria or cancer cells. In this review, we summarize the recent fluorescent probes described for the visualization of cell viability and some applications for in vivo imaging. On the other hand, positron emission tomography (PET) is a nuclear-based molecular imaging modality of great value for studying the activity of enzymes in vivo. We provide some examples of PET probes for imaging acetylcholinesterases and butyrylcholinesterases in the brain, which are valuable tools for diagnosing dementia and monitoring the effects of anticholinergic drugs on the central nervous system. MDPI 2023-08-27 /pmc/articles/PMC10488407/ /pubmed/37687094 http://dx.doi.org/10.3390/molecules28176265 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Gil-Rivas, Alba de Pascual-Teresa, Beatriz Ortín, Irene Ramos, Ana New Advances in the Exploration of Esterases with PET and Fluorescent Probes |
title | New Advances in the Exploration of Esterases with PET and Fluorescent Probes |
title_full | New Advances in the Exploration of Esterases with PET and Fluorescent Probes |
title_fullStr | New Advances in the Exploration of Esterases with PET and Fluorescent Probes |
title_full_unstemmed | New Advances in the Exploration of Esterases with PET and Fluorescent Probes |
title_short | New Advances in the Exploration of Esterases with PET and Fluorescent Probes |
title_sort | new advances in the exploration of esterases with pet and fluorescent probes |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488407/ https://www.ncbi.nlm.nih.gov/pubmed/37687094 http://dx.doi.org/10.3390/molecules28176265 |
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