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Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment

Background: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high est...

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Autores principales: Chrysanthopoulos, Ioannis, Mavrogianni, Despoina, Drakaki, Eirini, Potiris, Anastasios, Zikopoulos, Athanasios, Zachariou, Athanasios, Domali, Ekaterini, Drakakis, Peter, Stavros, Sofoklis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488692/
https://www.ncbi.nlm.nih.gov/pubmed/37685721
http://dx.doi.org/10.3390/jcm12175652
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author Chrysanthopoulos, Ioannis
Mavrogianni, Despoina
Drakaki, Eirini
Potiris, Anastasios
Zikopoulos, Athanasios
Zachariou, Athanasios
Domali, Ekaterini
Drakakis, Peter
Stavros, Sofoklis
author_facet Chrysanthopoulos, Ioannis
Mavrogianni, Despoina
Drakaki, Eirini
Potiris, Anastasios
Zikopoulos, Athanasios
Zachariou, Athanasios
Domali, Ekaterini
Drakakis, Peter
Stavros, Sofoklis
author_sort Chrysanthopoulos, Ioannis
collection PubMed
description Background: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high estrogen levels, we performed a direct analysis of granulosa cell samples to determine whether there are any significant changes in zeb1 expression during folliculogenesis. Methods: ZEB1 expression levels were measured in the granulosa cells of 56 infertile women undergoing IVF treatment. RNA extraction from granulosa cells was performed along with reverse transcription quantitative polymerase chain reaction (RT-qPCR) with SYBR Green I to determine zeb1 gene expression levels. Statistical analysis was performed by using t-test, while possible correlations of the expression of ZEB1 protein with body mass index (BMI), age, number of oocytes, and oocyte maturation were investigated. Results: Zeb1 gene expression levels correlate significantly with body mass index (BMI) and age, but not with oocyte number and oocyte maturation stage. Obese women demonstrate a higher expression level of zeb1 gene compared to normal and overweight women. Moreover, zeb1 gene is overexpressed in women aged 35–40 years old and is under-expressed in women >40 years old. Conclusions: ZEB1 expression should be further investigated as it may unveil new potential findings of the zeb1 gene’s role in female fertility and its use as a biomarker in fertility workups.
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spelling pubmed-104886922023-09-09 Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment Chrysanthopoulos, Ioannis Mavrogianni, Despoina Drakaki, Eirini Potiris, Anastasios Zikopoulos, Athanasios Zachariou, Athanasios Domali, Ekaterini Drakakis, Peter Stavros, Sofoklis J Clin Med Article Background: ZEB1 plays a role in epithelial-to-mesenchymal transition and acts as a repressor of E-cadherin, TGF-β, and Wnt/β-catenin. Since ZEB1 protein is expressed in estrogen-responsive tissues, and expression of the gene in the normal ovary and endometrium is positively correlated with high estrogen levels, we performed a direct analysis of granulosa cell samples to determine whether there are any significant changes in zeb1 expression during folliculogenesis. Methods: ZEB1 expression levels were measured in the granulosa cells of 56 infertile women undergoing IVF treatment. RNA extraction from granulosa cells was performed along with reverse transcription quantitative polymerase chain reaction (RT-qPCR) with SYBR Green I to determine zeb1 gene expression levels. Statistical analysis was performed by using t-test, while possible correlations of the expression of ZEB1 protein with body mass index (BMI), age, number of oocytes, and oocyte maturation were investigated. Results: Zeb1 gene expression levels correlate significantly with body mass index (BMI) and age, but not with oocyte number and oocyte maturation stage. Obese women demonstrate a higher expression level of zeb1 gene compared to normal and overweight women. Moreover, zeb1 gene is overexpressed in women aged 35–40 years old and is under-expressed in women >40 years old. Conclusions: ZEB1 expression should be further investigated as it may unveil new potential findings of the zeb1 gene’s role in female fertility and its use as a biomarker in fertility workups. MDPI 2023-08-30 /pmc/articles/PMC10488692/ /pubmed/37685721 http://dx.doi.org/10.3390/jcm12175652 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chrysanthopoulos, Ioannis
Mavrogianni, Despoina
Drakaki, Eirini
Potiris, Anastasios
Zikopoulos, Athanasios
Zachariou, Athanasios
Domali, Ekaterini
Drakakis, Peter
Stavros, Sofoklis
Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
title Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
title_full Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
title_fullStr Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
title_full_unstemmed Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
title_short Detection of zeb1 Gene in Granulosa Cells in Women Undergoing IVF Treatment
title_sort detection of zeb1 gene in granulosa cells in women undergoing ivf treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10488692/
https://www.ncbi.nlm.nih.gov/pubmed/37685721
http://dx.doi.org/10.3390/jcm12175652
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