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Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract
Alzheimer’s (AD) and Parkinson’s diseases (PD) are multifactorial neurogenerative disorders of the Central Nervous System causing severe cognitive and motor deficits in elderly people. Because treatment of AD and PD by synthetic drugs alleviates the symptoms often inducing side effects, many studies...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489013/ https://www.ncbi.nlm.nih.gov/pubmed/37687161 http://dx.doi.org/10.3390/molecules28176332 |
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author | Arcone, Rosaria D’Errico, Antonio Nasso, Rosarita Rullo, Rosario Poli, Annarita Di Donato, Paola Masullo, Mariorosario |
author_facet | Arcone, Rosaria D’Errico, Antonio Nasso, Rosarita Rullo, Rosario Poli, Annarita Di Donato, Paola Masullo, Mariorosario |
author_sort | Arcone, Rosaria |
collection | PubMed |
description | Alzheimer’s (AD) and Parkinson’s diseases (PD) are multifactorial neurogenerative disorders of the Central Nervous System causing severe cognitive and motor deficits in elderly people. Because treatment of AD and PD by synthetic drugs alleviates the symptoms often inducing side effects, many studies have aimed to find neuroprotective properties of diet polyphenols, compounds known to act on different cell signaling pathways. In this article, we analyzed the effect of polyphenols obtained from the agro-food industry waste of Citrus limon peel (LPE) on key enzymes of cholinergic and aminergic neurotransmission, such as butyryl cholinesterase (BuChE) and monoamine oxidases (MAO)-A/B, on Aβ(1–40) aggregation and on superoxide dismutase (SOD) 1/2 that affect oxidative stress. In our in vitro assays, LPE acts as an enzyme inhibitor on BuChE (IC(50) ~ 73 µM), MAO-A/B (IC(50) ~ 80 µM), SOD 1/2 (IC(50) ~ 10–20 µM) and interferes with Aβ(1–40) peptide aggregation (IC(50) ~ 170 µM). These results demonstrate that LPE behaves as a multitargeting agent against key factors of AD and PD by inhibiting to various extents BuChE, MAOs, and SODs and reducing Aβ-fibril aggregation. Therefore, LPE is a promising candidate for the prevention and management of AD and PD symptoms in combination with pharmacological therapies. |
format | Online Article Text |
id | pubmed-10489013 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104890132023-09-09 Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract Arcone, Rosaria D’Errico, Antonio Nasso, Rosarita Rullo, Rosario Poli, Annarita Di Donato, Paola Masullo, Mariorosario Molecules Article Alzheimer’s (AD) and Parkinson’s diseases (PD) are multifactorial neurogenerative disorders of the Central Nervous System causing severe cognitive and motor deficits in elderly people. Because treatment of AD and PD by synthetic drugs alleviates the symptoms often inducing side effects, many studies have aimed to find neuroprotective properties of diet polyphenols, compounds known to act on different cell signaling pathways. In this article, we analyzed the effect of polyphenols obtained from the agro-food industry waste of Citrus limon peel (LPE) on key enzymes of cholinergic and aminergic neurotransmission, such as butyryl cholinesterase (BuChE) and monoamine oxidases (MAO)-A/B, on Aβ(1–40) aggregation and on superoxide dismutase (SOD) 1/2 that affect oxidative stress. In our in vitro assays, LPE acts as an enzyme inhibitor on BuChE (IC(50) ~ 73 µM), MAO-A/B (IC(50) ~ 80 µM), SOD 1/2 (IC(50) ~ 10–20 µM) and interferes with Aβ(1–40) peptide aggregation (IC(50) ~ 170 µM). These results demonstrate that LPE behaves as a multitargeting agent against key factors of AD and PD by inhibiting to various extents BuChE, MAOs, and SODs and reducing Aβ-fibril aggregation. Therefore, LPE is a promising candidate for the prevention and management of AD and PD symptoms in combination with pharmacological therapies. MDPI 2023-08-30 /pmc/articles/PMC10489013/ /pubmed/37687161 http://dx.doi.org/10.3390/molecules28176332 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Arcone, Rosaria D’Errico, Antonio Nasso, Rosarita Rullo, Rosario Poli, Annarita Di Donato, Paola Masullo, Mariorosario Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract |
title | Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract |
title_full | Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract |
title_fullStr | Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract |
title_full_unstemmed | Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract |
title_short | Inhibition of Enzymes Involved in Neurodegenerative Disorders and Aβ(1–40) Aggregation by Citrus limon Peel Polyphenol Extract |
title_sort | inhibition of enzymes involved in neurodegenerative disorders and aβ(1–40) aggregation by citrus limon peel polyphenol extract |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489013/ https://www.ncbi.nlm.nih.gov/pubmed/37687161 http://dx.doi.org/10.3390/molecules28176332 |
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