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A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione

In this paper, by modifying the thioxanthene-benzothiozolium fluorophore, BCy-Cys, a lysosome-targeted near-infrared (NIR) fluorescent probe was synthesized for the detection of cysteine (Cys) from homocysteine (Hcy)/glutathione (GSH). As expected, BCy-Cys exhibited high selectivity and high sensiti...

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Autores principales: Liu, Qiuchen, Liu, Chang, He, Song, Zeng, Xianshun, Zhang, Jian, Gong, Jin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489057/
https://www.ncbi.nlm.nih.gov/pubmed/37687018
http://dx.doi.org/10.3390/molecules28176189
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author Liu, Qiuchen
Liu, Chang
He, Song
Zeng, Xianshun
Zhang, Jian
Gong, Jin
author_facet Liu, Qiuchen
Liu, Chang
He, Song
Zeng, Xianshun
Zhang, Jian
Gong, Jin
author_sort Liu, Qiuchen
collection PubMed
description In this paper, by modifying the thioxanthene-benzothiozolium fluorophore, BCy-Cys, a lysosome-targeted near-infrared (NIR) fluorescent probe was synthesized for the detection of cysteine (Cys) from homocysteine (Hcy)/glutathione (GSH). As expected, BCy-Cys exhibited high selectivity and high sensitivity for detection of Cys over Hcy/GSH, with an extremely low limit of detection at 0.31 μM, marked by obvious color changes. HRMS was conducted to confirm that the fluorescence intensity at 795 nm was significantly enhanced by the enhancement of intramolecular charge transfer (ICT). Importantly, BCy-Cys could be used to visualize both exogenous and endogenous lysosomal Cys, signifying its potential application in complex organismal systems.
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spelling pubmed-104890572023-09-09 A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione Liu, Qiuchen Liu, Chang He, Song Zeng, Xianshun Zhang, Jian Gong, Jin Molecules Article In this paper, by modifying the thioxanthene-benzothiozolium fluorophore, BCy-Cys, a lysosome-targeted near-infrared (NIR) fluorescent probe was synthesized for the detection of cysteine (Cys) from homocysteine (Hcy)/glutathione (GSH). As expected, BCy-Cys exhibited high selectivity and high sensitivity for detection of Cys over Hcy/GSH, with an extremely low limit of detection at 0.31 μM, marked by obvious color changes. HRMS was conducted to confirm that the fluorescence intensity at 795 nm was significantly enhanced by the enhancement of intramolecular charge transfer (ICT). Importantly, BCy-Cys could be used to visualize both exogenous and endogenous lysosomal Cys, signifying its potential application in complex organismal systems. MDPI 2023-08-22 /pmc/articles/PMC10489057/ /pubmed/37687018 http://dx.doi.org/10.3390/molecules28176189 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Qiuchen
Liu, Chang
He, Song
Zeng, Xianshun
Zhang, Jian
Gong, Jin
A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione
title A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione
title_full A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione
title_fullStr A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione
title_full_unstemmed A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione
title_short A New Lysosome-Targeted NIR Fluorescent Probe for Specific Detection of Cysteine over Homocysteine and Glutathione
title_sort new lysosome-targeted nir fluorescent probe for specific detection of cysteine over homocysteine and glutathione
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489057/
https://www.ncbi.nlm.nih.gov/pubmed/37687018
http://dx.doi.org/10.3390/molecules28176189
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