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Methodological Principles of Nasal Food Challenge

Thanks to their valuable assessment possibilities (subjective complaints and changes in nasal patency during the examination), nasal provocation tests may serve as an alternative tool for oral food challenges in the future. However, this test requires successive attempts to regulate its methodology...

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Autores principales: Krzych-Fałta, Edyta, Czerwińska, Monika E., Białek, Sławomir, Furmańczyk, Konrad, Samoliński, Bolesław, Grodner, Błażej, Sybilski, Adam, Nowicka, Grażyna, Wojas, Oksana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489609/
https://www.ncbi.nlm.nih.gov/pubmed/37686848
http://dx.doi.org/10.3390/nu15173816
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author Krzych-Fałta, Edyta
Czerwińska, Monika E.
Białek, Sławomir
Furmańczyk, Konrad
Samoliński, Bolesław
Grodner, Błażej
Sybilski, Adam
Nowicka, Grażyna
Wojas, Oksana
author_facet Krzych-Fałta, Edyta
Czerwińska, Monika E.
Białek, Sławomir
Furmańczyk, Konrad
Samoliński, Bolesław
Grodner, Błażej
Sybilski, Adam
Nowicka, Grażyna
Wojas, Oksana
author_sort Krzych-Fałta, Edyta
collection PubMed
description Thanks to their valuable assessment possibilities (subjective complaints and changes in nasal patency during the examination), nasal provocation tests may serve as an alternative tool for oral food challenges in the future. However, this test requires successive attempts to regulate its methodology in order to develop a standardized lyophilisate form and determine the threshold dose for a positive result. The study objective was to present the methodological foundation for nasal food allergen provocation tests induced by freeze-dried powdered chicken egg whites. A control group of 25 individuals with no history of allergy to chicken eggs or any other allergy was included in the study. Optical rhinometry and visual analog scales were used to assess the response of nasal mucosa to local allergen challenges. Minor variations in nasal flows, as measured by optical rhinometry, were observed in the provocation tests. The mean optical density measurements (as measured regardless of the allergen dose used) varied from positive to negative values and vice versa, e.g., amounting to 0.018 OD (standard deviation 0.095) at 15 min and −0.011 OD (standard deviation 0.090) at 30 min. No significant differences were observed concerning the perceived nasal discomfort using the visual analog scale. Due to the absence of nasal mucosal reactivity, nasal challenge is an excellent methodological tool for implementing food allergen tests.
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spelling pubmed-104896092023-09-09 Methodological Principles of Nasal Food Challenge Krzych-Fałta, Edyta Czerwińska, Monika E. Białek, Sławomir Furmańczyk, Konrad Samoliński, Bolesław Grodner, Błażej Sybilski, Adam Nowicka, Grażyna Wojas, Oksana Nutrients Article Thanks to their valuable assessment possibilities (subjective complaints and changes in nasal patency during the examination), nasal provocation tests may serve as an alternative tool for oral food challenges in the future. However, this test requires successive attempts to regulate its methodology in order to develop a standardized lyophilisate form and determine the threshold dose for a positive result. The study objective was to present the methodological foundation for nasal food allergen provocation tests induced by freeze-dried powdered chicken egg whites. A control group of 25 individuals with no history of allergy to chicken eggs or any other allergy was included in the study. Optical rhinometry and visual analog scales were used to assess the response of nasal mucosa to local allergen challenges. Minor variations in nasal flows, as measured by optical rhinometry, were observed in the provocation tests. The mean optical density measurements (as measured regardless of the allergen dose used) varied from positive to negative values and vice versa, e.g., amounting to 0.018 OD (standard deviation 0.095) at 15 min and −0.011 OD (standard deviation 0.090) at 30 min. No significant differences were observed concerning the perceived nasal discomfort using the visual analog scale. Due to the absence of nasal mucosal reactivity, nasal challenge is an excellent methodological tool for implementing food allergen tests. MDPI 2023-08-31 /pmc/articles/PMC10489609/ /pubmed/37686848 http://dx.doi.org/10.3390/nu15173816 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krzych-Fałta, Edyta
Czerwińska, Monika E.
Białek, Sławomir
Furmańczyk, Konrad
Samoliński, Bolesław
Grodner, Błażej
Sybilski, Adam
Nowicka, Grażyna
Wojas, Oksana
Methodological Principles of Nasal Food Challenge
title Methodological Principles of Nasal Food Challenge
title_full Methodological Principles of Nasal Food Challenge
title_fullStr Methodological Principles of Nasal Food Challenge
title_full_unstemmed Methodological Principles of Nasal Food Challenge
title_short Methodological Principles of Nasal Food Challenge
title_sort methodological principles of nasal food challenge
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489609/
https://www.ncbi.nlm.nih.gov/pubmed/37686848
http://dx.doi.org/10.3390/nu15173816
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