Cargando…

Interface-Based Design of High-Affinity Affibody Ligands for the Purification of RBD from Spike Proteins

The outbreak of coronavirus disease 2019 (COVID-19) has sparked an urgent demand for advanced diagnosis and vaccination worldwide. The discovery of high-affinity ligands is of great significance for vaccine and diagnostic reagent manufacturing. Targeting the receptor binding domain (RBD) from the sp...

Descripción completa

Detalles Bibliográficos
Autores principales:	Song, Siyuan, Shi, Qinghong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2023
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489752/ https://www.ncbi.nlm.nih.gov/pubmed/37687186 http://dx.doi.org/10.3390/molecules28176358

Ejemplares similares

Affibody-Binding Ligands
por: Barozzi, Annalisa, et al.
Publicado: (2020)

Expression, purification and characterization of SARS-CoV-2 spike RBD in ExpiCHO cells
por: De March, Matteo, et al.
Publicado: (2022)

Effects of common mutations in the SARS-CoV-2 Spike RBD and its ligand, the human ACE2 receptor on binding affinity and kinetics
por: Barton, Michael I, et al.
Publicado: (2021)

Mutational scanning of spike RBD protein for enhanced ACE2 affinity emerging Southeast Asia in the late transmission phase
por: Kodchakorn, Kanchanok, et al.
Publicado: (2022)

Implementation of Novel Affinity Ligand for Lentiviral Vector Purification
por: Moreira, Ana Sofia, et al.
Publicado: (2023)

Simultaneous targeting of two ligand-binding sites on VEGFR2 using biparatopic Affibody molecules results in dramatically improved affinity
por: Fleetwood, Filippa, et al.
Publicado: (2014)

Recombinant amyloid beta-peptide production by coexpression with an affibody ligand
por: Macao, Bertil, et al.
Publicado: (2008)

Effect of Delta and Omicron Mutations on the RBD-SD1 Domain of the Spike Protein in SARS-CoV-2 and the Omicron Mutations on RBD-ACE2 Interface Complex
por: Ching, Wai-Yim, et al.
Publicado: (2022)

Molecular design of radiocopper-labelled Affibody molecules
por: Tolmachev, Vladimir, et al.
Publicado: (2018)

Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy
por: Xue, Aiying, et al.
Publicado: (2022)

Large-Scale Purification and Characterization of Recombinant Receptor-Binding Domain (RBD) of SARS-CoV-2 Spike Protein Expressed in Yeast
por: Nagar, Gaurav, et al.
Publicado: (2023)

Affibody molecules as engineered protein drugs
por: Frejd, Fredrik Y, et al.
Publicado: (2017)

High-throughput Purification of Affinity-tagged Recombinant Proteins
por: Wiesler, Simone C., et al.
Publicado: (2012)

Affinity Membranes and Monoliths for Protein Purification
por: Lalli, Eleonora, et al.
Publicado: (2019)

Advances in Purification of SARS-CoV-2 Spike Ectodomain Protein Using High-Throughput Screening and Non-Affinity Methods
por: Cibelli, Nicole L., et al.
Publicado: (2021)

Advances in purification of SARS-CoV-2 spike ectodomain protein using high-throughput screening and non-affinity methods
por: Cibelli, Nicole, et al.
Publicado: (2022)

Targeted Delivery to Tumor-associated Pericytes via an Affibody with High Affinity for PDGFRβ Enhances the in vivo Antitumor Effects of Human TRAIL
por: Tao, Ze, et al.
Publicado: (2017)

Bionics design of affinity peptide inhibitors for SARS-CoV-2 RBD to block SARS-CoV-2 RBD-ACE2 interactions
por: Liu, Xiaofeng, et al.
Publicado: (2023)

Characterization of AAV-Specific Affinity Ligands: Consequences for Vector Purification and Development Strategies
por: Mietzsch, Mario, et al.
Publicado: (2020)

Multiepitope Proteins for the Differential Detection of IgG Antibodies against RBD of the Spike Protein and Non-RBD Regions of SARS-CoV-2
por: Gomes, Larissa R., et al.
Publicado: (2021)

Identification of sitagliptin binding proteins by affinity purification mass spectrometry: Sitagliptin binding proteins identified by affinity purification mass spectrometry
por: Wang, Xue-Ning, et al.
Publicado: (2022)

Engineering defensin α‐helix to produce high‐affinity SARS‐CoV‐2 spike protein binding ligands
por: Fernandes, Leonardo Antônio, et al.
Publicado: (2022)

Prokaryotic expression of antibodies and affibodies
por: Fernández, Luis Angel
Publicado: (2004)

Design and Synthesis of a Chitodisaccharide-Based Affinity Resin for Chitosanases Purification
por: Li, Shangyong, et al.
Publicado: (2019)

Structure-Based Epitope Design: Toward a Greater Antibody–SARS-CoV-2 RBD Affinity
por: Traboulsi, Hassan, et al.
Publicado: (2021)

Molecular dynamics study on the strengthening behavior of Delta and Omicron SARS-CoV-2 spike RBD improved receptor-binding affinity
por: Kodchakorn, Kanchanok, et al.
Publicado: (2022)

Novel Affibody Molecules Specifically Bind to SARS-CoV-2 Spike Protein and Efficiently Neutralize Delta and Omicron Variants
por: Du, Wangqi, et al.
Publicado: (2022)

Discovery of High-Affinity Protein Binding Ligands – Backwards
por: Diehnelt, Chris W., et al.
Publicado: (2010)

Novel, provable algorithms for efficient ensemble-based computational protein design and their application to the redesign of the c-Raf-RBD:KRas protein-protein interface
por: Lowegard, Anna U., et al.
Publicado: (2020)

Affinity Purification of an Archaeal DNA Replication Protein Network
por: Li, Zhuo, et al.
Publicado: (2010)

A Specific Interface between Integrin Transmembrane Helices and Affinity for Ligand
por: Luo, Bing-Hao, et al.
Publicado: (2004)

Non-Affinity Purification of Antibodies
por: Arakawa, Tsutomu, et al.
Publicado: (2023)

Computational prediction of the effect of amino acid changes on the binding affinity between SARS-CoV-2 spike RBD and human ACE2
por: Chen, Chen, et al.
Publicado: (2021)

A CNN model for predicting binding affinity changes between SARS-CoV-2 spike RBD variants and ACE2 homologues
por: Chen, Chen, et al.
Publicado: (2022)

A magnetic bead immunoassay to detect high affinity human IgG reactive to SARS-CoV-2 Spike S1 RBD produced in Escherichia coli
por: Conzentino, Marcelo S., et al.
Publicado: (2022)

Next generation calmodulin affinity purification: Clickable calmodulin facilitates improved protein purification
por: Fraseur, Julia G., et al.
Publicado: (2018)

Accessing three-branched high-affinity cereblon ligands for molecular glue and protein degrader design
por: Kuchta, Robert, et al.
Publicado: (2023)

Structure-guided design of a high-affinity ligand for a riboswitch
por: Huang, Lin, et al.
Publicado: (2019)

SARS-CoV-2 Variants, RBD Mutations, Binding Affinity, and Antibody Escape
por: Yang, Lin, et al.
Publicado: (2021)

Focused peptide library screening as a route to a superior affinity ligand for antibody purification
por: Bozovičar, Krištof, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_3843ve652d7j81ktd17fdqttbp