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Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts
The thermal behavior and aggregation process of the poly(N-isopropyl acrylamide), poly[oligo(ethylene glycol) methyl ether methacrylate], and poly[(2-hydroxyethyl methacrylate)-co-oligo(ethylene glycol) methyl ether methacrylate] thermoresponsive polymers were studied in a commonly used Dulbecco’s M...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489804/ https://www.ncbi.nlm.nih.gov/pubmed/37688213 http://dx.doi.org/10.3390/polym15173587 |
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author | Otulakowski, Łukasz Trzebicka, Barbara |
author_facet | Otulakowski, Łukasz Trzebicka, Barbara |
author_sort | Otulakowski, Łukasz |
collection | PubMed |
description | The thermal behavior and aggregation process of the poly(N-isopropyl acrylamide), poly[oligo(ethylene glycol) methyl ether methacrylate], and poly[(2-hydroxyethyl methacrylate)-co-oligo(ethylene glycol) methyl ether methacrylate] thermoresponsive polymers were studied in a commonly used Dulbecco’s Modified Eagle Medium (DMEM) cell culture medium and solutions of its individual components in the same concentration as found in DMEM. All studied copolymers exhibited an unexpected transmittance profile in the DMEM. During heating above the cloud point temperature (T(CP)), the polymers additionally aggregated, which led to the formation of their precipitates. The behavior of the polymers was further studied to evaluate how individual salts affected the transition temperature, size (D(h)), and stability of the polymer particles. Organic additives, such as amino acids and glucose, had a significantly lesser impact on the thermoresponsive aggregation of the polymers than inorganic ones. Changes to the T(CP) were small and the formation of precipitates was not observed. The presence of small amounts of amino acids caused a decrease in the polymer aggregate sizes. Obtained results are of utmost importance in thermoresponsive drug nanocarrier studies. |
format | Online Article Text |
id | pubmed-10489804 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104898042023-09-09 Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts Otulakowski, Łukasz Trzebicka, Barbara Polymers (Basel) Article The thermal behavior and aggregation process of the poly(N-isopropyl acrylamide), poly[oligo(ethylene glycol) methyl ether methacrylate], and poly[(2-hydroxyethyl methacrylate)-co-oligo(ethylene glycol) methyl ether methacrylate] thermoresponsive polymers were studied in a commonly used Dulbecco’s Modified Eagle Medium (DMEM) cell culture medium and solutions of its individual components in the same concentration as found in DMEM. All studied copolymers exhibited an unexpected transmittance profile in the DMEM. During heating above the cloud point temperature (T(CP)), the polymers additionally aggregated, which led to the formation of their precipitates. The behavior of the polymers was further studied to evaluate how individual salts affected the transition temperature, size (D(h)), and stability of the polymer particles. Organic additives, such as amino acids and glucose, had a significantly lesser impact on the thermoresponsive aggregation of the polymers than inorganic ones. Changes to the T(CP) were small and the formation of precipitates was not observed. The presence of small amounts of amino acids caused a decrease in the polymer aggregate sizes. Obtained results are of utmost importance in thermoresponsive drug nanocarrier studies. MDPI 2023-08-29 /pmc/articles/PMC10489804/ /pubmed/37688213 http://dx.doi.org/10.3390/polym15173587 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Otulakowski, Łukasz Trzebicka, Barbara Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts |
title | Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts |
title_full | Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts |
title_fullStr | Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts |
title_full_unstemmed | Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts |
title_short | Aggregation of Thermoresponsive Polymethacrylates in a Dulbecco’s Modified Eagle Medium and Its Salts |
title_sort | aggregation of thermoresponsive polymethacrylates in a dulbecco’s modified eagle medium and its salts |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489804/ https://www.ncbi.nlm.nih.gov/pubmed/37688213 http://dx.doi.org/10.3390/polym15173587 |
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