Cargando…

A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice

A high phosphorus intake has been associated with various metabolic disorders, including chronic kidney disease, cardiovascular disease, and osteoporosis. Recent studies have demonstrated the effects of dietary phosphorus on lipid and glucose metabolism. This study investigated the impact of a high-...

Descripción completa

Detalles Bibliográficos
Autores principales: Grundmann, Sarah M., Ress, Kerstin, Zimmermann, Lea, Höring, Marcus, Liebisch, Gerhard, Most, Erika, Ringseis, Robert, Eder, Klaus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489812/
https://www.ncbi.nlm.nih.gov/pubmed/37686765
http://dx.doi.org/10.3390/nu15173734
_version_ 1785103700885241856
author Grundmann, Sarah M.
Ress, Kerstin
Zimmermann, Lea
Höring, Marcus
Liebisch, Gerhard
Most, Erika
Ringseis, Robert
Eder, Klaus
author_facet Grundmann, Sarah M.
Ress, Kerstin
Zimmermann, Lea
Höring, Marcus
Liebisch, Gerhard
Most, Erika
Ringseis, Robert
Eder, Klaus
author_sort Grundmann, Sarah M.
collection PubMed
description A high phosphorus intake has been associated with various metabolic disorders, including chronic kidney disease, cardiovascular disease, and osteoporosis. Recent studies have demonstrated the effects of dietary phosphorus on lipid and glucose metabolism. This study investigated the impact of a high-phosphorus diet on mouse skeletal muscle lipid composition and gene transcription. Adult male mice (n = 12/group) received either a diet with an adequate (0.3%) or a high (1.2%) phosphorus concentration for 6 weeks. The lipidome analysis showed that among the 17 analyzed lipid classes, the concentrations of three classes were reduced in the high phosphorus group compared to the adequate phosphorus group. These classes were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and lysophosphatidylcholine (LPC) (p < 0.05). Out of the three hundred and twenty-three individual lipid species analyzed, forty-nine showed reduced concentrations, while three showed increased concentrations in the high phosphorus group compared to the adequate phosphorus group. The muscle transcriptome analysis identified 142 up- and 222 down-regulated transcripts in the high phosphorus group compared to the adequate phosphorus group. Gene set enrichment analysis identified that genes that were up-regulated in the high phosphorus group were linked to the gene ontology terms “mitochondria” and “Notch signaling pathway”, whereas genes that were down-regulated were linked to the “PI3K-AKT pathway”. Overall, the effects of the high-phosphorus diet on the muscle lipidome and transcriptome were relatively modest, but consistently indicated an impact on lipid metabolism.
format Online
Article
Text
id pubmed-10489812
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104898122023-09-09 A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice Grundmann, Sarah M. Ress, Kerstin Zimmermann, Lea Höring, Marcus Liebisch, Gerhard Most, Erika Ringseis, Robert Eder, Klaus Nutrients Article A high phosphorus intake has been associated with various metabolic disorders, including chronic kidney disease, cardiovascular disease, and osteoporosis. Recent studies have demonstrated the effects of dietary phosphorus on lipid and glucose metabolism. This study investigated the impact of a high-phosphorus diet on mouse skeletal muscle lipid composition and gene transcription. Adult male mice (n = 12/group) received either a diet with an adequate (0.3%) or a high (1.2%) phosphorus concentration for 6 weeks. The lipidome analysis showed that among the 17 analyzed lipid classes, the concentrations of three classes were reduced in the high phosphorus group compared to the adequate phosphorus group. These classes were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and lysophosphatidylcholine (LPC) (p < 0.05). Out of the three hundred and twenty-three individual lipid species analyzed, forty-nine showed reduced concentrations, while three showed increased concentrations in the high phosphorus group compared to the adequate phosphorus group. The muscle transcriptome analysis identified 142 up- and 222 down-regulated transcripts in the high phosphorus group compared to the adequate phosphorus group. Gene set enrichment analysis identified that genes that were up-regulated in the high phosphorus group were linked to the gene ontology terms “mitochondria” and “Notch signaling pathway”, whereas genes that were down-regulated were linked to the “PI3K-AKT pathway”. Overall, the effects of the high-phosphorus diet on the muscle lipidome and transcriptome were relatively modest, but consistently indicated an impact on lipid metabolism. MDPI 2023-08-25 /pmc/articles/PMC10489812/ /pubmed/37686765 http://dx.doi.org/10.3390/nu15173734 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Grundmann, Sarah M.
Ress, Kerstin
Zimmermann, Lea
Höring, Marcus
Liebisch, Gerhard
Most, Erika
Ringseis, Robert
Eder, Klaus
A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice
title A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice
title_full A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice
title_fullStr A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice
title_full_unstemmed A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice
title_short A High-Phosphorus Diet Moderately Alters the Lipidome and Transcriptome in the Skeletal Muscle of Adult Mice
title_sort high-phosphorus diet moderately alters the lipidome and transcriptome in the skeletal muscle of adult mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489812/
https://www.ncbi.nlm.nih.gov/pubmed/37686765
http://dx.doi.org/10.3390/nu15173734
work_keys_str_mv AT grundmannsarahm ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT resskerstin ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT zimmermannlea ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT horingmarcus ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT liebischgerhard ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT mosterika ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT ringseisrobert ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT ederklaus ahighphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT grundmannsarahm highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT resskerstin highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT zimmermannlea highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT horingmarcus highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT liebischgerhard highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT mosterika highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT ringseisrobert highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice
AT ederklaus highphosphorusdietmoderatelyaltersthelipidomeandtranscriptomeintheskeletalmuscleofadultmice