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Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo

Among all strategies directed at developing new tools to support re-vascularization of damaged tissues, the use of pro-angiogenic soluble factors, derived from mesenchymal stem cells (MSCs), appears a promising approach for regenerative medicine. Here, we compared the feasibility of two devices, gen...

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Autores principales: Barone, Ludovica, Gallazzi, Matteo, Rossi, Federica, Papait, Roberto, Raspanti, Mario, Zecca, Piero Antonio, Buonarrivo, Luca, Bassani, Barbara, Bernardini, Giovanni, Bruno, Antonino, Gornati, Rosalba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489993/
https://www.ncbi.nlm.nih.gov/pubmed/37686988
http://dx.doi.org/10.3390/nano13172479
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author Barone, Ludovica
Gallazzi, Matteo
Rossi, Federica
Papait, Roberto
Raspanti, Mario
Zecca, Piero Antonio
Buonarrivo, Luca
Bassani, Barbara
Bernardini, Giovanni
Bruno, Antonino
Gornati, Rosalba
author_facet Barone, Ludovica
Gallazzi, Matteo
Rossi, Federica
Papait, Roberto
Raspanti, Mario
Zecca, Piero Antonio
Buonarrivo, Luca
Bassani, Barbara
Bernardini, Giovanni
Bruno, Antonino
Gornati, Rosalba
author_sort Barone, Ludovica
collection PubMed
description Among all strategies directed at developing new tools to support re-vascularization of damaged tissues, the use of pro-angiogenic soluble factors, derived from mesenchymal stem cells (MSCs), appears a promising approach for regenerative medicine. Here, we compared the feasibility of two devices, generated by coupling soluble factors of human dental pulp mesenchymal stem cells (DPSCs), with a nanostructured scaffold, to support angiogenesis once transplanted in mice. DPSCs were obtained from impacted wisdom tooth removal, usually considered surgical waste material. After 28 days, we verified the presence of active blood vessels inside the scaffold through optical and scansion electron microscopy. The mRNA expression of surface antigens related to macrophage polarization (CD68, CD80, CD86, CD163, CD206), as well as pro-angiogenic markers (CD31, CD34, CD105, Angpt1, Angpt2, CDH5) was evaluated by real-time PCR. Our results demonstrate the capability of DPSC–scaffold and DPSC soluble factors–scaffold to support angiogenesis, similarly to adipose stem cells, whereas the absence of blood vessels was found in the scaffold grafted alone. Our results provide evidence that DPSC-conditioned medium can be proposed as a cell-free preparation able to support angiogenesis, thus, providing a relevant tool to overcome the issues and restrictions associated with the use of cells.
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spelling pubmed-104899932023-09-09 Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo Barone, Ludovica Gallazzi, Matteo Rossi, Federica Papait, Roberto Raspanti, Mario Zecca, Piero Antonio Buonarrivo, Luca Bassani, Barbara Bernardini, Giovanni Bruno, Antonino Gornati, Rosalba Nanomaterials (Basel) Article Among all strategies directed at developing new tools to support re-vascularization of damaged tissues, the use of pro-angiogenic soluble factors, derived from mesenchymal stem cells (MSCs), appears a promising approach for regenerative medicine. Here, we compared the feasibility of two devices, generated by coupling soluble factors of human dental pulp mesenchymal stem cells (DPSCs), with a nanostructured scaffold, to support angiogenesis once transplanted in mice. DPSCs were obtained from impacted wisdom tooth removal, usually considered surgical waste material. After 28 days, we verified the presence of active blood vessels inside the scaffold through optical and scansion electron microscopy. The mRNA expression of surface antigens related to macrophage polarization (CD68, CD80, CD86, CD163, CD206), as well as pro-angiogenic markers (CD31, CD34, CD105, Angpt1, Angpt2, CDH5) was evaluated by real-time PCR. Our results demonstrate the capability of DPSC–scaffold and DPSC soluble factors–scaffold to support angiogenesis, similarly to adipose stem cells, whereas the absence of blood vessels was found in the scaffold grafted alone. Our results provide evidence that DPSC-conditioned medium can be proposed as a cell-free preparation able to support angiogenesis, thus, providing a relevant tool to overcome the issues and restrictions associated with the use of cells. MDPI 2023-09-02 /pmc/articles/PMC10489993/ /pubmed/37686988 http://dx.doi.org/10.3390/nano13172479 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Barone, Ludovica
Gallazzi, Matteo
Rossi, Federica
Papait, Roberto
Raspanti, Mario
Zecca, Piero Antonio
Buonarrivo, Luca
Bassani, Barbara
Bernardini, Giovanni
Bruno, Antonino
Gornati, Rosalba
Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
title Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
title_full Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
title_fullStr Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
title_full_unstemmed Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
title_short Human Dental Pulp Mesenchymal Stem Cell-Derived Soluble Factors Combined with a Nanostructured Scaffold Support the Generation of a Vascular Network In Vivo
title_sort human dental pulp mesenchymal stem cell-derived soluble factors combined with a nanostructured scaffold support the generation of a vascular network in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10489993/
https://www.ncbi.nlm.nih.gov/pubmed/37686988
http://dx.doi.org/10.3390/nano13172479
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