Cargando…

Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton

Receptor-like cytoplasmic kinase VII (RLCK-VII) subfamily members are vital players in plant innate immunity and are also involved in plant development and abiotic stress tolerance. As a widely cultivated textile crop, upland cotton (Gossypium hirsutum) attaches great importance to the cotton indust...

Descripción completa

Detalles Bibliográficos
Autores principales: Cen, Yuhan, Geng, Shiyi, Gao, Linying, Wang, Xinyue, Yan, Xin, Hou, Yuxia, Wang, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490013/
https://www.ncbi.nlm.nih.gov/pubmed/37687414
http://dx.doi.org/10.3390/plants12173170
_version_ 1785103742617518080
author Cen, Yuhan
Geng, Shiyi
Gao, Linying
Wang, Xinyue
Yan, Xin
Hou, Yuxia
Wang, Ping
author_facet Cen, Yuhan
Geng, Shiyi
Gao, Linying
Wang, Xinyue
Yan, Xin
Hou, Yuxia
Wang, Ping
author_sort Cen, Yuhan
collection PubMed
description Receptor-like cytoplasmic kinase VII (RLCK-VII) subfamily members are vital players in plant innate immunity and are also involved in plant development and abiotic stress tolerance. As a widely cultivated textile crop, upland cotton (Gossypium hirsutum) attaches great importance to the cotton industry worldwide. To obtain details of the composition, phylogeny, and putative function of RLCK-VII genes in upland cotton, genome-wide identification, evolutionary event analysis, and expression pattern examination of RLCK-VII subfamily genes in G. hirsutum were performed. There are 129 RLCK-VII members in upland cotton (GhRLCKs) and they were divided into nine groups based on their phylogenetic relationships. The gene structure and sequence features are relatively conserved within each group, which were divided based on their phylogenetic relationships, and consistent with those in Arabidopsis. The phylogenetic analysis results showed that RLCK-VII subfamily genes evolved in plants before the speciation of Arabidopsis and cotton, and segmental duplication was the major factor that caused the expansion of GhRLCKs. The diverse expression patterns of GhRLCKs in response to abiotic stresses (temperature, salt, and drought) and V. dahliae infection were observed. The candidates that may be involved in cotton’s response to these stresses are highlighted. GhRLCK7 (GhRLCK7A and D), which is notably induced by V. dahliae infection, was demonstrated to positively regulate cotton defense against V. dahliae by the loss-of-function assay in cotton. These findings shed light on the details of the RLCK-VII subfamily in cotton and provide a scaffold for the further function elucidation and application of GhRLCKs for the germplasm innovation of cotton.
format Online
Article
Text
id pubmed-10490013
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-104900132023-09-09 Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton Cen, Yuhan Geng, Shiyi Gao, Linying Wang, Xinyue Yan, Xin Hou, Yuxia Wang, Ping Plants (Basel) Article Receptor-like cytoplasmic kinase VII (RLCK-VII) subfamily members are vital players in plant innate immunity and are also involved in plant development and abiotic stress tolerance. As a widely cultivated textile crop, upland cotton (Gossypium hirsutum) attaches great importance to the cotton industry worldwide. To obtain details of the composition, phylogeny, and putative function of RLCK-VII genes in upland cotton, genome-wide identification, evolutionary event analysis, and expression pattern examination of RLCK-VII subfamily genes in G. hirsutum were performed. There are 129 RLCK-VII members in upland cotton (GhRLCKs) and they were divided into nine groups based on their phylogenetic relationships. The gene structure and sequence features are relatively conserved within each group, which were divided based on their phylogenetic relationships, and consistent with those in Arabidopsis. The phylogenetic analysis results showed that RLCK-VII subfamily genes evolved in plants before the speciation of Arabidopsis and cotton, and segmental duplication was the major factor that caused the expansion of GhRLCKs. The diverse expression patterns of GhRLCKs in response to abiotic stresses (temperature, salt, and drought) and V. dahliae infection were observed. The candidates that may be involved in cotton’s response to these stresses are highlighted. GhRLCK7 (GhRLCK7A and D), which is notably induced by V. dahliae infection, was demonstrated to positively regulate cotton defense against V. dahliae by the loss-of-function assay in cotton. These findings shed light on the details of the RLCK-VII subfamily in cotton and provide a scaffold for the further function elucidation and application of GhRLCKs for the germplasm innovation of cotton. MDPI 2023-09-04 /pmc/articles/PMC10490013/ /pubmed/37687414 http://dx.doi.org/10.3390/plants12173170 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cen, Yuhan
Geng, Shiyi
Gao, Linying
Wang, Xinyue
Yan, Xin
Hou, Yuxia
Wang, Ping
Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton
title Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton
title_full Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton
title_fullStr Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton
title_full_unstemmed Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton
title_short Genome-Wide Identification and Expression Analysis of RLCK-VII Subfamily Genes Reveal Their Roles in Stress Responses of Upland Cotton
title_sort genome-wide identification and expression analysis of rlck-vii subfamily genes reveal their roles in stress responses of upland cotton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490013/
https://www.ncbi.nlm.nih.gov/pubmed/37687414
http://dx.doi.org/10.3390/plants12173170
work_keys_str_mv AT cenyuhan genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton
AT gengshiyi genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton
AT gaolinying genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton
AT wangxinyue genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton
AT yanxin genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton
AT houyuxia genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton
AT wangping genomewideidentificationandexpressionanalysisofrlckviisubfamilygenesrevealtheirrolesinstressresponsesofuplandcotton