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Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng
Panax ginseng, renowned for its medicinal properties, relies on adventitious roots and hairy roots as crucial sources for the production of ginsenosides. Despite the widespread utilization of ginseng, investigations into its miRNAs have remained scarce. To address this gap, two samples of ginseng ad...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490192/ https://www.ncbi.nlm.nih.gov/pubmed/37687317 http://dx.doi.org/10.3390/plants12173070 |
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author | Liu, Chang Jiang, Yang Yun, Ziyi Zhang, Kexin Zhao, Mingzhu Wang, Yi Zhang, Meiping Tian, Zhuo Wang, Kangyu |
author_facet | Liu, Chang Jiang, Yang Yun, Ziyi Zhang, Kexin Zhao, Mingzhu Wang, Yi Zhang, Meiping Tian, Zhuo Wang, Kangyu |
author_sort | Liu, Chang |
collection | PubMed |
description | Panax ginseng, renowned for its medicinal properties, relies on adventitious roots and hairy roots as crucial sources for the production of ginsenosides. Despite the widespread utilization of ginseng, investigations into its miRNAs have remained scarce. To address this gap, two samples of ginseng adventitious roots and ginseng hairy roots were collected, and subsequent construction and sequencing of small RNA libraries of ginseng adventitious roots and hairy roots were performed using the Illumina HiSeq X Ten platform. The analysis of the sequencing data unveiled total miRNAs 2432. The miR166 and miR396 were the most highly expressed miRNA families in ginseng. The miRNA expression analysis results were used to validate the qRT-PCR. Target genes of miRNA were predicted and GO function annotation and KEGG pathway analysis were performed on target genes. It was found that miRNAs are mainly involved in synthetic pathways and biological processes in plants, which include metabolic and bioregulatory processes. The plant miRNAs enriched KEGG pathways are associated with some metabolism, especially amino acid metabolism and carbohydrate metabolism. These results provide valuable insights miRNAs and their roles in metabolic processes in ginseng. |
format | Online Article Text |
id | pubmed-10490192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-104901922023-09-09 Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng Liu, Chang Jiang, Yang Yun, Ziyi Zhang, Kexin Zhao, Mingzhu Wang, Yi Zhang, Meiping Tian, Zhuo Wang, Kangyu Plants (Basel) Article Panax ginseng, renowned for its medicinal properties, relies on adventitious roots and hairy roots as crucial sources for the production of ginsenosides. Despite the widespread utilization of ginseng, investigations into its miRNAs have remained scarce. To address this gap, two samples of ginseng adventitious roots and ginseng hairy roots were collected, and subsequent construction and sequencing of small RNA libraries of ginseng adventitious roots and hairy roots were performed using the Illumina HiSeq X Ten platform. The analysis of the sequencing data unveiled total miRNAs 2432. The miR166 and miR396 were the most highly expressed miRNA families in ginseng. The miRNA expression analysis results were used to validate the qRT-PCR. Target genes of miRNA were predicted and GO function annotation and KEGG pathway analysis were performed on target genes. It was found that miRNAs are mainly involved in synthetic pathways and biological processes in plants, which include metabolic and bioregulatory processes. The plant miRNAs enriched KEGG pathways are associated with some metabolism, especially amino acid metabolism and carbohydrate metabolism. These results provide valuable insights miRNAs and their roles in metabolic processes in ginseng. MDPI 2023-08-27 /pmc/articles/PMC10490192/ /pubmed/37687317 http://dx.doi.org/10.3390/plants12173070 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Liu, Chang Jiang, Yang Yun, Ziyi Zhang, Kexin Zhao, Mingzhu Wang, Yi Zhang, Meiping Tian, Zhuo Wang, Kangyu Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng |
title | Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng |
title_full | Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng |
title_fullStr | Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng |
title_full_unstemmed | Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng |
title_short | Small RNA-Seq to Unveil the miRNA Expression Patterns and Identify the Target Genes in Panax ginseng |
title_sort | small rna-seq to unveil the mirna expression patterns and identify the target genes in panax ginseng |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490192/ https://www.ncbi.nlm.nih.gov/pubmed/37687317 http://dx.doi.org/10.3390/plants12173070 |
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