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Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)

Blackleg disease is devastating for wasabi (Eutrema japonicum) production, occurring at any time and everywhere within the main production area of the Sichuan Province, China. There have been very few studies on the chemical control of this disease. In this study, we isolated and identified a local...

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Autores principales: Liu, Yanjun, Song, Changjiang, Ren, Xin, Wu, Guoli, Ma, Zihan, Zhao, Mantong, Xie, Yujia, Li, Yu, Lai, Yunsong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490250/
https://www.ncbi.nlm.nih.gov/pubmed/37687395
http://dx.doi.org/10.3390/plants12173149
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author Liu, Yanjun
Song, Changjiang
Ren, Xin
Wu, Guoli
Ma, Zihan
Zhao, Mantong
Xie, Yujia
Li, Yu
Lai, Yunsong
author_facet Liu, Yanjun
Song, Changjiang
Ren, Xin
Wu, Guoli
Ma, Zihan
Zhao, Mantong
Xie, Yujia
Li, Yu
Lai, Yunsong
author_sort Liu, Yanjun
collection PubMed
description Blackleg disease is devastating for wasabi (Eutrema japonicum) production, occurring at any time and everywhere within the main production area of the Sichuan Province, China. There have been very few studies on the chemical control of this disease. In this study, we isolated and identified a local popular strain of the pathogen Plenodomus wasabiae. The isolated fungus strain caused typical disease spots on the leaves and rhizomes upon inoculation back to wasabi seedlings. The symptoms of blackleg disease developed very quickly, becaming visible on the second day after exposure to P. wasabiae and leading to death within one week. We then evaluated the efficacy of ten widely used fungicides to screen out effective fungicides. The efficacy of the tested fungicides was determined through mycelial growth inhibition on medium plates. As a result, tebuconazole and pyraclostrobin were able to inhibit the mycelial growth of P. wasabiae, and the most widely used dimethomorph in local production areas produced the lowest inhibition activity (13.8%). Nevertheless, the highest control efficacy of tebuconazole and pyraclostrobin on wasabi seedlings was only 47.48% and 39.03%, respectively. Generally, the control efficacy of spraying the fungicide before inoculation was better than that after inoculation. An increase in the application concentration of the two fungicides did not proportionately result in improved performance. We cloned the full-length sequence of sterol 14-demethylase (CYP51) and cytochrome B (CYTB) of which the mutations may contribute to the possible antifungalresistance. These two genes of the isolated fungus do not possess any reported mutations that lead to fungicide resistance. Previous studies indicate that there is a significant difference between fungicides in terms of the effectiveness of controlling blackleg disease; however, the control efficacy of fungicides is limited in blackleg control. Therefore, field management to prevent wound infection and unfavorable environmental conditions are more important than pesticide management.
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spelling pubmed-104902502023-09-09 Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum) Liu, Yanjun Song, Changjiang Ren, Xin Wu, Guoli Ma, Zihan Zhao, Mantong Xie, Yujia Li, Yu Lai, Yunsong Plants (Basel) Article Blackleg disease is devastating for wasabi (Eutrema japonicum) production, occurring at any time and everywhere within the main production area of the Sichuan Province, China. There have been very few studies on the chemical control of this disease. In this study, we isolated and identified a local popular strain of the pathogen Plenodomus wasabiae. The isolated fungus strain caused typical disease spots on the leaves and rhizomes upon inoculation back to wasabi seedlings. The symptoms of blackleg disease developed very quickly, becaming visible on the second day after exposure to P. wasabiae and leading to death within one week. We then evaluated the efficacy of ten widely used fungicides to screen out effective fungicides. The efficacy of the tested fungicides was determined through mycelial growth inhibition on medium plates. As a result, tebuconazole and pyraclostrobin were able to inhibit the mycelial growth of P. wasabiae, and the most widely used dimethomorph in local production areas produced the lowest inhibition activity (13.8%). Nevertheless, the highest control efficacy of tebuconazole and pyraclostrobin on wasabi seedlings was only 47.48% and 39.03%, respectively. Generally, the control efficacy of spraying the fungicide before inoculation was better than that after inoculation. An increase in the application concentration of the two fungicides did not proportionately result in improved performance. We cloned the full-length sequence of sterol 14-demethylase (CYP51) and cytochrome B (CYTB) of which the mutations may contribute to the possible antifungalresistance. These two genes of the isolated fungus do not possess any reported mutations that lead to fungicide resistance. Previous studies indicate that there is a significant difference between fungicides in terms of the effectiveness of controlling blackleg disease; however, the control efficacy of fungicides is limited in blackleg control. Therefore, field management to prevent wound infection and unfavorable environmental conditions are more important than pesticide management. MDPI 2023-09-01 /pmc/articles/PMC10490250/ /pubmed/37687395 http://dx.doi.org/10.3390/plants12173149 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Yanjun
Song, Changjiang
Ren, Xin
Wu, Guoli
Ma, Zihan
Zhao, Mantong
Xie, Yujia
Li, Yu
Lai, Yunsong
Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)
title Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)
title_full Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)
title_fullStr Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)
title_full_unstemmed Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)
title_short Screening for Fungicide Efficacy in Controlling Blackleg Disease in Wasabi (Eutrema japonicum)
title_sort screening for fungicide efficacy in controlling blackleg disease in wasabi (eutrema japonicum)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490250/
https://www.ncbi.nlm.nih.gov/pubmed/37687395
http://dx.doi.org/10.3390/plants12173149
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