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Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment

Using cytotoxic reducing and stabilizing agents in the synthesis of gold nanoparticles (AuNPs) limits their use in biomedical applications. One strategy to overcome this problem is using “green” synthesis methodologies using polysaccharides. In the present study, we propose a green methodology for s...

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Autores principales: Pinilla-Torres, Ana M., Sanchez-Dominguez, Celia N., Basilio-Bernabe, Karla, Carrion-Garcia, Paola Y., Roacho-Perez, Jorge A., Garza-Treviño, Elsa N., Gallardo-Blanco, Hugo, Sanchez-Dominguez, Margarita
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490394/
https://www.ncbi.nlm.nih.gov/pubmed/37688159
http://dx.doi.org/10.3390/polym15173533
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author Pinilla-Torres, Ana M.
Sanchez-Dominguez, Celia N.
Basilio-Bernabe, Karla
Carrion-Garcia, Paola Y.
Roacho-Perez, Jorge A.
Garza-Treviño, Elsa N.
Gallardo-Blanco, Hugo
Sanchez-Dominguez, Margarita
author_facet Pinilla-Torres, Ana M.
Sanchez-Dominguez, Celia N.
Basilio-Bernabe, Karla
Carrion-Garcia, Paola Y.
Roacho-Perez, Jorge A.
Garza-Treviño, Elsa N.
Gallardo-Blanco, Hugo
Sanchez-Dominguez, Margarita
author_sort Pinilla-Torres, Ana M.
collection PubMed
description Using cytotoxic reducing and stabilizing agents in the synthesis of gold nanoparticles (AuNPs) limits their use in biomedical applications. One strategy to overcome this problem is using “green” synthesis methodologies using polysaccharides. In the present study, we propose a green methodology for synthetizing AuNPs with mesquite gum (MG) as a reducing agent and steric stabilizer in Gold(III) chloride trihydrate aqueous solutions to obtain biocompatible nanoparticles that can be used for biomedical applications. Through this method, AuNPs can be produced without using elevated temperatures or pressures. For synthetizing gold nanoparticles coated with mesquite gum (AuNPs@MG), Gold(III) chloride trihydrate was used as a precursor, and mesquite gum was used as a stabilizing and reducing agent. The AuNPs obtained were characterized using UV-Vis spectroscopy, dynamic light scattering, transmission electron microscopy, scanning transmission electron microscopy, and FT-IR spectroscopy. The stability in biological media (phosphate buffer solution), cytotoxicity (MTT assay, hematoxylin, and eosin staining), and hemocompatibility (Hemolysis assay) were measured at different concentrations and exposure times. The results showed the successful synthesis of AuNPs@MG with sizes ranging from 3 to 30 nm and a zeta potential of −31 mV. The AuNPs@MG showed good colloidal stability in PBS (pH 7.4) for up to 24 h. Finally, cytotoxicity assays showed no changes in cell metabolism or cell morphology. These results suggest that these gold nanoparticles have potential biomedical applications because of their low cytotoxicity and hemotoxicity and improved stability at a physiological pH.
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spelling pubmed-104903942023-09-09 Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment Pinilla-Torres, Ana M. Sanchez-Dominguez, Celia N. Basilio-Bernabe, Karla Carrion-Garcia, Paola Y. Roacho-Perez, Jorge A. Garza-Treviño, Elsa N. Gallardo-Blanco, Hugo Sanchez-Dominguez, Margarita Polymers (Basel) Article Using cytotoxic reducing and stabilizing agents in the synthesis of gold nanoparticles (AuNPs) limits their use in biomedical applications. One strategy to overcome this problem is using “green” synthesis methodologies using polysaccharides. In the present study, we propose a green methodology for synthetizing AuNPs with mesquite gum (MG) as a reducing agent and steric stabilizer in Gold(III) chloride trihydrate aqueous solutions to obtain biocompatible nanoparticles that can be used for biomedical applications. Through this method, AuNPs can be produced without using elevated temperatures or pressures. For synthetizing gold nanoparticles coated with mesquite gum (AuNPs@MG), Gold(III) chloride trihydrate was used as a precursor, and mesquite gum was used as a stabilizing and reducing agent. The AuNPs obtained were characterized using UV-Vis spectroscopy, dynamic light scattering, transmission electron microscopy, scanning transmission electron microscopy, and FT-IR spectroscopy. The stability in biological media (phosphate buffer solution), cytotoxicity (MTT assay, hematoxylin, and eosin staining), and hemocompatibility (Hemolysis assay) were measured at different concentrations and exposure times. The results showed the successful synthesis of AuNPs@MG with sizes ranging from 3 to 30 nm and a zeta potential of −31 mV. The AuNPs@MG showed good colloidal stability in PBS (pH 7.4) for up to 24 h. Finally, cytotoxicity assays showed no changes in cell metabolism or cell morphology. These results suggest that these gold nanoparticles have potential biomedical applications because of their low cytotoxicity and hemotoxicity and improved stability at a physiological pH. MDPI 2023-08-24 /pmc/articles/PMC10490394/ /pubmed/37688159 http://dx.doi.org/10.3390/polym15173533 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pinilla-Torres, Ana M.
Sanchez-Dominguez, Celia N.
Basilio-Bernabe, Karla
Carrion-Garcia, Paola Y.
Roacho-Perez, Jorge A.
Garza-Treviño, Elsa N.
Gallardo-Blanco, Hugo
Sanchez-Dominguez, Margarita
Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment
title Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment
title_full Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment
title_fullStr Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment
title_full_unstemmed Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment
title_short Green Synthesis of Mesquite-Gum-Stabilized Gold Nanoparticles for Biomedical Applications: Physicochemical Properties and Biocompatibility Assessment
title_sort green synthesis of mesquite-gum-stabilized gold nanoparticles for biomedical applications: physicochemical properties and biocompatibility assessment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490394/
https://www.ncbi.nlm.nih.gov/pubmed/37688159
http://dx.doi.org/10.3390/polym15173533
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