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Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor

The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and the environment. However, the applications of modern biosensors face numerous problems and require improvement. Some of these problem...

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Autores principales: Generalov, Vladimir, Cheremiskina, Anastasia, Glukhov, Alexander, Grabezhova, Victoria, Kruchinina, Margarita, Safatov, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490761/
https://www.ncbi.nlm.nih.gov/pubmed/37687945
http://dx.doi.org/10.3390/s23177490
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author Generalov, Vladimir
Cheremiskina, Anastasia
Glukhov, Alexander
Grabezhova, Victoria
Kruchinina, Margarita
Safatov, Alexander
author_facet Generalov, Vladimir
Cheremiskina, Anastasia
Glukhov, Alexander
Grabezhova, Victoria
Kruchinina, Margarita
Safatov, Alexander
author_sort Generalov, Vladimir
collection PubMed
description The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and the environment. However, the applications of modern biosensors face numerous problems and require improvement. Some of these problems can be attributed to sensor design, while others can be attributed to technological limitations. The aim of this work is to conduct a theoretical investigation of the “antibody + antigen” complex (AB + AG) detection processes of a SOI-FET biosensor, which may also solve some of the aforementioned problems. Our investigation concentrates on the analysis of the probability of AB + AG complex detection and evaluation. Poisson probability density distribution was used to estimate the probability of the adsorption of the target molecules on the biosensor’s surface and, consequently, to obtain correct detection results. Many implicit and unexpected causes of error detection have been identified for AB + AG complexes using SOI-FET biosensors. We showed that accuracy and time of detection depend on the number of SOI-FET biosensors on a crystal.
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spelling pubmed-104907612023-09-09 Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor Generalov, Vladimir Cheremiskina, Anastasia Glukhov, Alexander Grabezhova, Victoria Kruchinina, Margarita Safatov, Alexander Sensors (Basel) Article The SOI-FET biosensor (silicon-on-insulator field-effect transistor) for virus detection is a promising device in the fields of medicine, virology, biotechnology, and the environment. However, the applications of modern biosensors face numerous problems and require improvement. Some of these problems can be attributed to sensor design, while others can be attributed to technological limitations. The aim of this work is to conduct a theoretical investigation of the “antibody + antigen” complex (AB + AG) detection processes of a SOI-FET biosensor, which may also solve some of the aforementioned problems. Our investigation concentrates on the analysis of the probability of AB + AG complex detection and evaluation. Poisson probability density distribution was used to estimate the probability of the adsorption of the target molecules on the biosensor’s surface and, consequently, to obtain correct detection results. Many implicit and unexpected causes of error detection have been identified for AB + AG complexes using SOI-FET biosensors. We showed that accuracy and time of detection depend on the number of SOI-FET biosensors on a crystal. MDPI 2023-08-29 /pmc/articles/PMC10490761/ /pubmed/37687945 http://dx.doi.org/10.3390/s23177490 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Generalov, Vladimir
Cheremiskina, Anastasia
Glukhov, Alexander
Grabezhova, Victoria
Kruchinina, Margarita
Safatov, Alexander
Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor
title Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor
title_full Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor
title_fullStr Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor
title_full_unstemmed Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor
title_short Investigation of Limitations in the Detection of Antibody + Antigen Complexes Using the Silicon-on-Insulator Field-Effect Transistor Biosensor
title_sort investigation of limitations in the detection of antibody + antigen complexes using the silicon-on-insulator field-effect transistor biosensor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490761/
https://www.ncbi.nlm.nih.gov/pubmed/37687945
http://dx.doi.org/10.3390/s23177490
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