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A computer vision based optical method for measuring fluid level in cell culture plates

For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or...

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Detalles Bibliográficos
Autores principales: Baudin, Pierre V., Teodorescu, Mircea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490923/
https://www.ncbi.nlm.nih.gov/pubmed/37682933
http://dx.doi.org/10.1371/journal.pone.0290951
Descripción
Sumario:For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.