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A computer vision based optical method for measuring fluid level in cell culture plates

For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or...

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Detalles Bibliográficos
Autores principales: Baudin, Pierre V., Teodorescu, Mircea
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490923/
https://www.ncbi.nlm.nih.gov/pubmed/37682933
http://dx.doi.org/10.1371/journal.pone.0290951
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author Baudin, Pierre V.
Teodorescu, Mircea
author_facet Baudin, Pierre V.
Teodorescu, Mircea
author_sort Baudin, Pierre V.
collection PubMed
description For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera.
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spelling pubmed-104909232023-09-09 A computer vision based optical method for measuring fluid level in cell culture plates Baudin, Pierre V. Teodorescu, Mircea PLoS One Research Article For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera. Public Library of Science 2023-09-08 /pmc/articles/PMC10490923/ /pubmed/37682933 http://dx.doi.org/10.1371/journal.pone.0290951 Text en © 2023 Baudin, Teodorescu https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Baudin, Pierre V.
Teodorescu, Mircea
A computer vision based optical method for measuring fluid level in cell culture plates
title A computer vision based optical method for measuring fluid level in cell culture plates
title_full A computer vision based optical method for measuring fluid level in cell culture plates
title_fullStr A computer vision based optical method for measuring fluid level in cell culture plates
title_full_unstemmed A computer vision based optical method for measuring fluid level in cell culture plates
title_short A computer vision based optical method for measuring fluid level in cell culture plates
title_sort computer vision based optical method for measuring fluid level in cell culture plates
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490923/
https://www.ncbi.nlm.nih.gov/pubmed/37682933
http://dx.doi.org/10.1371/journal.pone.0290951
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