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A computer vision based optical method for measuring fluid level in cell culture plates
For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490923/ https://www.ncbi.nlm.nih.gov/pubmed/37682933 http://dx.doi.org/10.1371/journal.pone.0290951 |
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author | Baudin, Pierre V. Teodorescu, Mircea |
author_facet | Baudin, Pierre V. Teodorescu, Mircea |
author_sort | Baudin, Pierre V. |
collection | PubMed |
description | For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera. |
format | Online Article Text |
id | pubmed-10490923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104909232023-09-09 A computer vision based optical method for measuring fluid level in cell culture plates Baudin, Pierre V. Teodorescu, Mircea PLoS One Research Article For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid’s index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera. Public Library of Science 2023-09-08 /pmc/articles/PMC10490923/ /pubmed/37682933 http://dx.doi.org/10.1371/journal.pone.0290951 Text en © 2023 Baudin, Teodorescu https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Baudin, Pierre V. Teodorescu, Mircea A computer vision based optical method for measuring fluid level in cell culture plates |
title | A computer vision based optical method for measuring fluid level in cell culture plates |
title_full | A computer vision based optical method for measuring fluid level in cell culture plates |
title_fullStr | A computer vision based optical method for measuring fluid level in cell culture plates |
title_full_unstemmed | A computer vision based optical method for measuring fluid level in cell culture plates |
title_short | A computer vision based optical method for measuring fluid level in cell culture plates |
title_sort | computer vision based optical method for measuring fluid level in cell culture plates |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490923/ https://www.ncbi.nlm.nih.gov/pubmed/37682933 http://dx.doi.org/10.1371/journal.pone.0290951 |
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