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Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
Interleukin-6 (IL-6) is a pleiotropic cytokine that coordinates host immune responses to infection. Though essential to the acute phase response, prolonged IL-6-mediated recruitment of mononuclear cells has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490926/ https://www.ncbi.nlm.nih.gov/pubmed/37682929 http://dx.doi.org/10.1371/journal.pone.0291076 |
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author | Duncan, John Benjamin W. Basu, Swarna Vivekanand, Pavithra |
author_facet | Duncan, John Benjamin W. Basu, Swarna Vivekanand, Pavithra |
author_sort | Duncan, John Benjamin W. |
collection | PubMed |
description | Interleukin-6 (IL-6) is a pleiotropic cytokine that coordinates host immune responses to infection. Though essential to the acute phase response, prolonged IL-6-mediated recruitment of mononuclear cells has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and Crohn’s disease. Accordingly, identifying novel therapeutics that diminish circulating IL-6 levels could benefit individuals suffering from chronic inflammation. In immunocompetent hosts, bacterial lipopolysaccharide (LPS) recognition by toll-like receptor 4 (TLR4) activates the transcription factor NF-κB, driving macrophage production of IL-6. Interestingly, both citrate-stabilized and ‘green’ synthesized gold nanoparticles (AuNPs) have been shown to modulate the cytokine responses of LPS-activated macrophages. Here we demonstrate that AuNPs, synthesized with commercial and locally sourced honey, downregulate LPS-induced macrophage secretion of IL-6. Compared to LPS-only controls, inhibition of IL-6 levels was observed for all three types of honey AuNPs. The effect was likely driven by honey AuNP-mediated perturbation of the TLR4/NF-κB signaling pathway, as evidenced by a reduction in the phosphorylation of IκB. Further investigation into the anti-inflammatory properties of honey AuNPs may yield novel therapeutics for the treatment of chronic inflammation. |
format | Online Article Text |
id | pubmed-10490926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-104909262023-09-09 Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages Duncan, John Benjamin W. Basu, Swarna Vivekanand, Pavithra PLoS One Research Article Interleukin-6 (IL-6) is a pleiotropic cytokine that coordinates host immune responses to infection. Though essential to the acute phase response, prolonged IL-6-mediated recruitment of mononuclear cells has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and Crohn’s disease. Accordingly, identifying novel therapeutics that diminish circulating IL-6 levels could benefit individuals suffering from chronic inflammation. In immunocompetent hosts, bacterial lipopolysaccharide (LPS) recognition by toll-like receptor 4 (TLR4) activates the transcription factor NF-κB, driving macrophage production of IL-6. Interestingly, both citrate-stabilized and ‘green’ synthesized gold nanoparticles (AuNPs) have been shown to modulate the cytokine responses of LPS-activated macrophages. Here we demonstrate that AuNPs, synthesized with commercial and locally sourced honey, downregulate LPS-induced macrophage secretion of IL-6. Compared to LPS-only controls, inhibition of IL-6 levels was observed for all three types of honey AuNPs. The effect was likely driven by honey AuNP-mediated perturbation of the TLR4/NF-κB signaling pathway, as evidenced by a reduction in the phosphorylation of IκB. Further investigation into the anti-inflammatory properties of honey AuNPs may yield novel therapeutics for the treatment of chronic inflammation. Public Library of Science 2023-09-08 /pmc/articles/PMC10490926/ /pubmed/37682929 http://dx.doi.org/10.1371/journal.pone.0291076 Text en © 2023 Duncan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Duncan, John Benjamin W. Basu, Swarna Vivekanand, Pavithra Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages |
title | Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages |
title_full | Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages |
title_fullStr | Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages |
title_full_unstemmed | Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages |
title_short | Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages |
title_sort | honey gold nanoparticles attenuate the secretion of il-6 by lps-activated macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490926/ https://www.ncbi.nlm.nih.gov/pubmed/37682929 http://dx.doi.org/10.1371/journal.pone.0291076 |
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