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Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages

Interleukin-6 (IL-6) is a pleiotropic cytokine that coordinates host immune responses to infection. Though essential to the acute phase response, prolonged IL-6-mediated recruitment of mononuclear cells has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and...

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Detalles Bibliográficos
Autores principales: Duncan, John Benjamin W., Basu, Swarna, Vivekanand, Pavithra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490926/
https://www.ncbi.nlm.nih.gov/pubmed/37682929
http://dx.doi.org/10.1371/journal.pone.0291076
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author Duncan, John Benjamin W.
Basu, Swarna
Vivekanand, Pavithra
author_facet Duncan, John Benjamin W.
Basu, Swarna
Vivekanand, Pavithra
author_sort Duncan, John Benjamin W.
collection PubMed
description Interleukin-6 (IL-6) is a pleiotropic cytokine that coordinates host immune responses to infection. Though essential to the acute phase response, prolonged IL-6-mediated recruitment of mononuclear cells has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and Crohn’s disease. Accordingly, identifying novel therapeutics that diminish circulating IL-6 levels could benefit individuals suffering from chronic inflammation. In immunocompetent hosts, bacterial lipopolysaccharide (LPS) recognition by toll-like receptor 4 (TLR4) activates the transcription factor NF-κB, driving macrophage production of IL-6. Interestingly, both citrate-stabilized and ‘green’ synthesized gold nanoparticles (AuNPs) have been shown to modulate the cytokine responses of LPS-activated macrophages. Here we demonstrate that AuNPs, synthesized with commercial and locally sourced honey, downregulate LPS-induced macrophage secretion of IL-6. Compared to LPS-only controls, inhibition of IL-6 levels was observed for all three types of honey AuNPs. The effect was likely driven by honey AuNP-mediated perturbation of the TLR4/NF-κB signaling pathway, as evidenced by a reduction in the phosphorylation of IκB. Further investigation into the anti-inflammatory properties of honey AuNPs may yield novel therapeutics for the treatment of chronic inflammation.
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spelling pubmed-104909262023-09-09 Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages Duncan, John Benjamin W. Basu, Swarna Vivekanand, Pavithra PLoS One Research Article Interleukin-6 (IL-6) is a pleiotropic cytokine that coordinates host immune responses to infection. Though essential to the acute phase response, prolonged IL-6-mediated recruitment of mononuclear cells has been implicated in chronic inflammatory diseases such as rheumatoid arthritis, psoriasis, and Crohn’s disease. Accordingly, identifying novel therapeutics that diminish circulating IL-6 levels could benefit individuals suffering from chronic inflammation. In immunocompetent hosts, bacterial lipopolysaccharide (LPS) recognition by toll-like receptor 4 (TLR4) activates the transcription factor NF-κB, driving macrophage production of IL-6. Interestingly, both citrate-stabilized and ‘green’ synthesized gold nanoparticles (AuNPs) have been shown to modulate the cytokine responses of LPS-activated macrophages. Here we demonstrate that AuNPs, synthesized with commercial and locally sourced honey, downregulate LPS-induced macrophage secretion of IL-6. Compared to LPS-only controls, inhibition of IL-6 levels was observed for all three types of honey AuNPs. The effect was likely driven by honey AuNP-mediated perturbation of the TLR4/NF-κB signaling pathway, as evidenced by a reduction in the phosphorylation of IκB. Further investigation into the anti-inflammatory properties of honey AuNPs may yield novel therapeutics for the treatment of chronic inflammation. Public Library of Science 2023-09-08 /pmc/articles/PMC10490926/ /pubmed/37682929 http://dx.doi.org/10.1371/journal.pone.0291076 Text en © 2023 Duncan et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Duncan, John Benjamin W.
Basu, Swarna
Vivekanand, Pavithra
Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
title Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
title_full Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
title_fullStr Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
title_full_unstemmed Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
title_short Honey gold nanoparticles attenuate the secretion of IL-6 by LPS-activated macrophages
title_sort honey gold nanoparticles attenuate the secretion of il-6 by lps-activated macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10490926/
https://www.ncbi.nlm.nih.gov/pubmed/37682929
http://dx.doi.org/10.1371/journal.pone.0291076
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