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Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus
Zebrafish are popular research organisms selected for laboratory use due in part to widespread availability from the pet trade. Many contemporary colonies of laboratory zebrafish are maintained in aquaculture facilities that monitor and aim to curb infections that can negatively affect colony health...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cold Spring Harbor Laboratory
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491165/ https://www.ncbi.nlm.nih.gov/pubmed/37693489 http://dx.doi.org/10.1101/2023.08.28.555169 |
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author | Rice, Marlen C. Elde, Nels C. Gagnon, James A. Balla, Keir M. |
author_facet | Rice, Marlen C. Elde, Nels C. Gagnon, James A. Balla, Keir M. |
author_sort | Rice, Marlen C. |
collection | PubMed |
description | Zebrafish are popular research organisms selected for laboratory use due in part to widespread availability from the pet trade. Many contemporary colonies of laboratory zebrafish are maintained in aquaculture facilities that monitor and aim to curb infections that can negatively affect colony health and confound experiments. The impact of laboratory control on the microbial constituents associated with zebrafish in research environments compared to the pet trade are unclear. Diseases of unknown causes are common in both environments. We conducted a metagenomic survey to broadly compare the zebrafish-associated microbes in pet trade and laboratory environments. We detected many microbes in animals from the pet trade that were not found in laboratory animals. Co-housing experiments revealed several transmissible microbes including a newly described non-enveloped, double-stranded RNA virus in the Birnaviridae family we name Rocky Mountain birnavirus (RMBV). Infections were detected in asymptomatic animals from the pet trade, but when transmitted to laboratory animals RMBV was associated with pronounced antiviral responses and hemorrhagic disease. These experiments highlight the pet trade as a distinct source of diverse microbes that associate with zebrafish and establish a paradigm for the discovery of newly described pathogenic viruses and other infectious microbes that can be developed for study in the laboratory. |
format | Online Article Text |
id | pubmed-10491165 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cold Spring Harbor Laboratory |
record_format | MEDLINE/PubMed |
spelling | pubmed-104911652023-09-09 Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus Rice, Marlen C. Elde, Nels C. Gagnon, James A. Balla, Keir M. bioRxiv Article Zebrafish are popular research organisms selected for laboratory use due in part to widespread availability from the pet trade. Many contemporary colonies of laboratory zebrafish are maintained in aquaculture facilities that monitor and aim to curb infections that can negatively affect colony health and confound experiments. The impact of laboratory control on the microbial constituents associated with zebrafish in research environments compared to the pet trade are unclear. Diseases of unknown causes are common in both environments. We conducted a metagenomic survey to broadly compare the zebrafish-associated microbes in pet trade and laboratory environments. We detected many microbes in animals from the pet trade that were not found in laboratory animals. Co-housing experiments revealed several transmissible microbes including a newly described non-enveloped, double-stranded RNA virus in the Birnaviridae family we name Rocky Mountain birnavirus (RMBV). Infections were detected in asymptomatic animals from the pet trade, but when transmitted to laboratory animals RMBV was associated with pronounced antiviral responses and hemorrhagic disease. These experiments highlight the pet trade as a distinct source of diverse microbes that associate with zebrafish and establish a paradigm for the discovery of newly described pathogenic viruses and other infectious microbes that can be developed for study in the laboratory. Cold Spring Harbor Laboratory 2023-08-28 /pmc/articles/PMC10491165/ /pubmed/37693489 http://dx.doi.org/10.1101/2023.08.28.555169 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Rice, Marlen C. Elde, Nels C. Gagnon, James A. Balla, Keir M. Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
title | Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
title_full | Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
title_fullStr | Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
title_full_unstemmed | Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
title_short | Microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
title_sort | microbe transmission from pet shop to lab-reared zebrafish reveals a pathogenic birnavirus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491165/ https://www.ncbi.nlm.nih.gov/pubmed/37693489 http://dx.doi.org/10.1101/2023.08.28.555169 |
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