Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells

Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. Here, we show that site-specific RNA breaks generated with RNA-targeting CRISPR complexes are repaired in huma...

Descripción completa

Detalles Bibliográficos
Autores principales: Nemudraia, Anna, Nemudryi, Artem, Wiedenheft, Blake
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491232/
https://www.ncbi.nlm.nih.gov/pubmed/37693568
http://dx.doi.org/10.1101/2023.08.29.555404
_version_ 1785104018656198656
author Nemudraia, Anna
Nemudryi, Artem
Wiedenheft, Blake
author_facet Nemudraia, Anna
Nemudryi, Artem
Wiedenheft, Blake
author_sort Nemudraia, Anna
collection PubMed
description Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. Here, we show that site-specific RNA breaks generated with RNA-targeting CRISPR complexes are repaired in human cells, and this repair can be used for programmable deletions in human transcripts that restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications.
format Online
Article
Text
id pubmed-10491232
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory
record_format MEDLINE/PubMed
spelling pubmed-104912322023-09-09 Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells Nemudraia, Anna Nemudryi, Artem Wiedenheft, Blake bioRxiv Article Genome editing with CRISPR RNA-guided endonucleases generates DNA breaks that are resolved by cellular DNA repair machinery. However, analogous methods to manipulate RNA remain unavailable. Here, we show that site-specific RNA breaks generated with RNA-targeting CRISPR complexes are repaired in human cells, and this repair can be used for programmable deletions in human transcripts that restore gene function. Collectively, this work establishes a technology for precise RNA manipulation with potential therapeutic applications. Cold Spring Harbor Laboratory 2023-08-29 /pmc/articles/PMC10491232/ /pubmed/37693568 http://dx.doi.org/10.1101/2023.08.29.555404 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Nemudraia, Anna
Nemudryi, Artem
Wiedenheft, Blake
Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells
title Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells
title_full Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells
title_fullStr Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells
title_full_unstemmed Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells
title_short Repair of CRISPR-guided RNA breaks enables site-specific RNA editing in human cells
title_sort repair of crispr-guided rna breaks enables site-specific rna editing in human cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491232/
https://www.ncbi.nlm.nih.gov/pubmed/37693568
http://dx.doi.org/10.1101/2023.08.29.555404
work_keys_str_mv AT nemudraiaanna repairofcrisprguidedrnabreaksenablessitespecificrnaeditinginhumancells
AT nemudryiartem repairofcrisprguidedrnabreaksenablessitespecificrnaeditinginhumancells
AT wiedenheftblake repairofcrisprguidedrnabreaksenablessitespecificrnaeditinginhumancells

Ejemplares similares