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Imaging structurally dynamic ribosomes with cryogenic electron microscopy
Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding of ribosomal biology. Here, we provide a historical perspective at the intersection of electron microscopy technology develop...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cornell University
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491319/ |
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author | Webster, Samantha M. May, Mira B. Powell, Barrett M. Davis, Joseph H. |
author_facet | Webster, Samantha M. May, Mira B. Powell, Barrett M. Davis, Joseph H. |
author_sort | Webster, Samantha M. |
collection | PubMed |
description | Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding of ribosomal biology. Here, we provide a historical perspective at the intersection of electron microscopy technology development and ribosome biology and reflect on how this technique has shed light on each stage of the life cycle of this dynamic macromolecular machine. With an emphasis on prokaryotic systems, we specifically describe how pairing cryo-EM with thoughtful experimental design, time-resolved techniques, and next-generation heterogeneous structural analysis has afforded insights into the modular nature of assembly, the roles of the many transient biogenesis and translation co-factors, and the subtle variations in structure and function between strains and species. This work concludes with a prospective outlook on the field, highlighting the pivotal role cryogenic electron tomography is playing in adding cellular context to our understanding of ribosomal life cycles, and noting how this exciting technology promises to bridge the gap between cellular and structural biology. |
format | Online Article Text |
id | pubmed-10491319 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cornell University |
record_format | MEDLINE/PubMed |
spelling | pubmed-104913192023-09-09 Imaging structurally dynamic ribosomes with cryogenic electron microscopy Webster, Samantha M. May, Mira B. Powell, Barrett M. Davis, Joseph H. ArXiv Article Throughout the history of electron microscopy, ribosomes have served as an ideal subject for imaging and technological development, which in turn has driven our understanding of ribosomal biology. Here, we provide a historical perspective at the intersection of electron microscopy technology development and ribosome biology and reflect on how this technique has shed light on each stage of the life cycle of this dynamic macromolecular machine. With an emphasis on prokaryotic systems, we specifically describe how pairing cryo-EM with thoughtful experimental design, time-resolved techniques, and next-generation heterogeneous structural analysis has afforded insights into the modular nature of assembly, the roles of the many transient biogenesis and translation co-factors, and the subtle variations in structure and function between strains and species. This work concludes with a prospective outlook on the field, highlighting the pivotal role cryogenic electron tomography is playing in adding cellular context to our understanding of ribosomal life cycles, and noting how this exciting technology promises to bridge the gap between cellular and structural biology. Cornell University 2023-08-30 /pmc/articles/PMC10491319/ Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which allows reusers to copy and distribute the material in any medium or format in unadapted form only, for noncommercial purposes only, and only so long as attribution is given to the creator. |
spellingShingle | Article Webster, Samantha M. May, Mira B. Powell, Barrett M. Davis, Joseph H. Imaging structurally dynamic ribosomes with cryogenic electron microscopy |
title | Imaging structurally dynamic ribosomes with cryogenic electron microscopy |
title_full | Imaging structurally dynamic ribosomes with cryogenic electron microscopy |
title_fullStr | Imaging structurally dynamic ribosomes with cryogenic electron microscopy |
title_full_unstemmed | Imaging structurally dynamic ribosomes with cryogenic electron microscopy |
title_short | Imaging structurally dynamic ribosomes with cryogenic electron microscopy |
title_sort | imaging structurally dynamic ribosomes with cryogenic electron microscopy |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491319/ |
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