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Protocol for high-throughput semi-automated label-free- or TMT-based phosphoproteome profiling

Tandem mass tags data-dependent acquisition (TMT-DDA) as well as data-independent acquisition-based label-free quantification (LFQ-DIA) have become the leading workflows to achieve deep proteome and phosphoproteome profiles. We present a modular pipeline for TMT-DDA and LFQ-DIA that integrates steps...

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Detalles Bibliográficos
Autores principales:	Koenig, Claire, Martinez-Val, Ana, Naicker, Previn, Stoychev, Stoyan, Jordaan, Justin, Olsen, Jesper V.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2023
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491724/ https://www.ncbi.nlm.nih.gov/pubmed/37659085 http://dx.doi.org/10.1016/j.xpro.2023.102536

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