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VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive

OBJECTIVE: To adapt to metabolically challenging environments, the central nervous system (CNS) orchestrates metabolism of peripheral organs including skeletal muscle. The organ-communication between the CNS and skeletal muscle has been investigated, yet our understanding of the neuronal pathway fro...

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Autores principales: Yoshida, Takuya, Fujitani, Mina, Farmer, Scotlynn, Harada, Ami, Shi, Zhen, Lee, Jenny J., Tinajero, Arely, Singha, Ashish K., Fujikawa, Teppei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491730/
https://www.ncbi.nlm.nih.gov/pubmed/37633515
http://dx.doi.org/10.1016/j.molmet.2023.101792
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author Yoshida, Takuya
Fujitani, Mina
Farmer, Scotlynn
Harada, Ami
Shi, Zhen
Lee, Jenny J.
Tinajero, Arely
Singha, Ashish K.
Fujikawa, Teppei
author_facet Yoshida, Takuya
Fujitani, Mina
Farmer, Scotlynn
Harada, Ami
Shi, Zhen
Lee, Jenny J.
Tinajero, Arely
Singha, Ashish K.
Fujikawa, Teppei
author_sort Yoshida, Takuya
collection PubMed
description OBJECTIVE: To adapt to metabolically challenging environments, the central nervous system (CNS) orchestrates metabolism of peripheral organs including skeletal muscle. The organ-communication between the CNS and skeletal muscle has been investigated, yet our understanding of the neuronal pathway from the CNS to skeletal muscle is still limited. Neurons in the dorsomedial and central parts of the ventromedial hypothalamic nucleus (VMHdm/c) expressing steroidogenic factor-1 (VMHdm/c(SF-1) neurons) are key for metabolic adaptations to exercise, including increased basal metabolic rate and skeletal muscle mass in mice. However, the mechanisms by which VMHdm/c(SF-1) neurons regulate skeletal muscle function remain unclear. Here, we show that VMHdm/c(SF-1) neurons increase the sympathoadrenal activity and regulate skeletal muscle peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) in mice via multiple downstream nodes. METHODS: Optogenetics was used to specifically manipulate VMHdm/c(SF-1) neurons combined with genetically-engineered mice and surgical manipulation of the sympathoadrenal activity. RESULTS: Optogenetic activation of VMHdm/c(SF-1) neurons dramatically elevates mRNA levels of skeletal muscle Pgc-1α, which regulates a spectrum of skeletal muscle function including protein synthesis and metabolism. Mechanistically, the sympathoadrenal drive coupled with β2 adrenergic receptor (β2AdR) is essential for VMHdm/c(SF-1) neurons-mediated increases in skeletal muscle PGC1-α. Specifically, both adrenalectomy and β2AdR knockout block augmented skeletal muscle PGC1-α by VMHdm/c(SF-1) neuronal activation. Optogenetic functional mapping reveals that downstream nodes of VMHdm/c(SF-1) neurons are functionally redundant to increase circulating epinephrine and skeletal muscle PGC1-α. CONCLUSIONS: Collectively, we propose that VMHdm/c(SF-1) neurons-skeletal muscle pathway, VMHdm/c(SF-1) neurons→multiple downstream nodes→the adrenal gland→skeletal muscle β2AdR, underlies augmented skeletal muscle function for metabolic adaptations.
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spelling pubmed-104917302023-09-10 VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive Yoshida, Takuya Fujitani, Mina Farmer, Scotlynn Harada, Ami Shi, Zhen Lee, Jenny J. Tinajero, Arely Singha, Ashish K. Fujikawa, Teppei Mol Metab Original Article OBJECTIVE: To adapt to metabolically challenging environments, the central nervous system (CNS) orchestrates metabolism of peripheral organs including skeletal muscle. The organ-communication between the CNS and skeletal muscle has been investigated, yet our understanding of the neuronal pathway from the CNS to skeletal muscle is still limited. Neurons in the dorsomedial and central parts of the ventromedial hypothalamic nucleus (VMHdm/c) expressing steroidogenic factor-1 (VMHdm/c(SF-1) neurons) are key for metabolic adaptations to exercise, including increased basal metabolic rate and skeletal muscle mass in mice. However, the mechanisms by which VMHdm/c(SF-1) neurons regulate skeletal muscle function remain unclear. Here, we show that VMHdm/c(SF-1) neurons increase the sympathoadrenal activity and regulate skeletal muscle peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) in mice via multiple downstream nodes. METHODS: Optogenetics was used to specifically manipulate VMHdm/c(SF-1) neurons combined with genetically-engineered mice and surgical manipulation of the sympathoadrenal activity. RESULTS: Optogenetic activation of VMHdm/c(SF-1) neurons dramatically elevates mRNA levels of skeletal muscle Pgc-1α, which regulates a spectrum of skeletal muscle function including protein synthesis and metabolism. Mechanistically, the sympathoadrenal drive coupled with β2 adrenergic receptor (β2AdR) is essential for VMHdm/c(SF-1) neurons-mediated increases in skeletal muscle PGC1-α. Specifically, both adrenalectomy and β2AdR knockout block augmented skeletal muscle PGC1-α by VMHdm/c(SF-1) neuronal activation. Optogenetic functional mapping reveals that downstream nodes of VMHdm/c(SF-1) neurons are functionally redundant to increase circulating epinephrine and skeletal muscle PGC1-α. CONCLUSIONS: Collectively, we propose that VMHdm/c(SF-1) neurons-skeletal muscle pathway, VMHdm/c(SF-1) neurons→multiple downstream nodes→the adrenal gland→skeletal muscle β2AdR, underlies augmented skeletal muscle function for metabolic adaptations. Elsevier 2023-08-24 /pmc/articles/PMC10491730/ /pubmed/37633515 http://dx.doi.org/10.1016/j.molmet.2023.101792 Text en © 2023 Published by Elsevier GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Yoshida, Takuya
Fujitani, Mina
Farmer, Scotlynn
Harada, Ami
Shi, Zhen
Lee, Jenny J.
Tinajero, Arely
Singha, Ashish K.
Fujikawa, Teppei
VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive
title VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive
title_full VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive
title_fullStr VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive
title_full_unstemmed VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive
title_short VMHdm/c(SF-1) neuronal circuits regulate skeletal muscle PGC1-α via the sympathoadrenal drive
title_sort vmhdm/c(sf-1) neuronal circuits regulate skeletal muscle pgc1-α via the sympathoadrenal drive
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491730/
https://www.ncbi.nlm.nih.gov/pubmed/37633515
http://dx.doi.org/10.1016/j.molmet.2023.101792
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