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Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation
Quantitative assessment of endogenously synthesized and released bilirubin from brain tissue remains a challenge. Here, we present a sensitive and reproducible experimental paradigm to quantify, in real time, unconjugated bilirubin (UCB) from isolated murine brain tissue during oxygen-glucose depriv...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491851/ https://www.ncbi.nlm.nih.gov/pubmed/37660297 http://dx.doi.org/10.1016/j.xpro.2023.102550 |
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author | Liu, Han-Wei Lai, Ke Gong, Li-Na Shi, Hai-Bo Yin, Shan-Kai Wang, Lu-Yang |
author_facet | Liu, Han-Wei Lai, Ke Gong, Li-Na Shi, Hai-Bo Yin, Shan-Kai Wang, Lu-Yang |
author_sort | Liu, Han-Wei |
collection | PubMed |
description | Quantitative assessment of endogenously synthesized and released bilirubin from brain tissue remains a challenge. Here, we present a sensitive and reproducible experimental paradigm to quantify, in real time, unconjugated bilirubin (UCB) from isolated murine brain tissue during oxygen-glucose deprivation (OGD). We describe steps for perfusion, brain dissection, brain slice preparation and incubation, glucose depletion, and OGD processing. We then detail procedures for standard calibration plotting and sample UCB measurement. For complete details on the use and execution of this protocol, please refer to Liu et al.(1) |
format | Online Article Text |
id | pubmed-10491851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-104918512023-09-10 Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation Liu, Han-Wei Lai, Ke Gong, Li-Na Shi, Hai-Bo Yin, Shan-Kai Wang, Lu-Yang STAR Protoc Protocol Quantitative assessment of endogenously synthesized and released bilirubin from brain tissue remains a challenge. Here, we present a sensitive and reproducible experimental paradigm to quantify, in real time, unconjugated bilirubin (UCB) from isolated murine brain tissue during oxygen-glucose deprivation (OGD). We describe steps for perfusion, brain dissection, brain slice preparation and incubation, glucose depletion, and OGD processing. We then detail procedures for standard calibration plotting and sample UCB measurement. For complete details on the use and execution of this protocol, please refer to Liu et al.(1) Elsevier 2023-09-02 /pmc/articles/PMC10491851/ /pubmed/37660297 http://dx.doi.org/10.1016/j.xpro.2023.102550 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Protocol Liu, Han-Wei Lai, Ke Gong, Li-Na Shi, Hai-Bo Yin, Shan-Kai Wang, Lu-Yang Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
title | Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
title_full | Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
title_fullStr | Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
title_full_unstemmed | Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
title_short | Measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
title_sort | measuring endogenous levels of unconjugated bilirubin released from isolated murine brain tissue during oxygen-glucose deprivation |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491851/ https://www.ncbi.nlm.nih.gov/pubmed/37660297 http://dx.doi.org/10.1016/j.xpro.2023.102550 |
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