Protocol for identifying immune checkpoint on circulating tumor cells of human pancreatic ductal adenocarcinoma by single-cell RNA sequencing

Circulating tumor cells (CTCs) are regarded as the “seeds” of tumor metastasis. Identifying immune checkpoints on CTCs is essential for establishing efficient immunotherapies to prevent tumor metastasis. Here, we present a protocol for isolating CTCs and obtaining single-cell suspensions from pancre...

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Detalles Bibliográficos
Autores principales: Liu, Xiaowei, Song, Jinen, Liu, Xinyu, Zhang, Hao, Wang, Xueyan, Li, Yuanxi, Yang, Zhankun, Jing, Jing, Ma, Xuelei, Shi, Hubing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10491853/
https://www.ncbi.nlm.nih.gov/pubmed/37659082
http://dx.doi.org/10.1016/j.xpro.2023.102539

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