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Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin
OBJECTIVES: To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects. MATERIALS AND METHODS: A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. Th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Springer Berlin Heidelberg
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10492744/ https://www.ncbi.nlm.nih.gov/pubmed/37421492 http://dx.doi.org/10.1007/s00784-023-05123-x |
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author | Chackartchi, Tali Bosshardt, Dieter D. Imber, Jean-Claude Stähli, Alexandra Sacks, Hagit Nagy, Katalin Sculean, Anton |
author_facet | Chackartchi, Tali Bosshardt, Dieter D. Imber, Jean-Claude Stähli, Alexandra Sacks, Hagit Nagy, Katalin Sculean, Anton |
author_sort | Chackartchi, Tali |
collection | PubMed |
description | OBJECTIVES: To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects. MATERIALS AND METHODS: A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated. RESULTS: The test group yielded statistically significantly (p = 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (p = 0.94). CONCLUSIONS: The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing. CLINICAL RELEVANCE: The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery. |
format | Online Article Text |
id | pubmed-10492744 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-104927442023-09-11 Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin Chackartchi, Tali Bosshardt, Dieter D. Imber, Jean-Claude Stähli, Alexandra Sacks, Hagit Nagy, Katalin Sculean, Anton Clin Oral Investig Research OBJECTIVES: To histologically evaluate the effects of a novel human recombinant amelogenin (rAmelX) on periodontal wound healing / regeneration in recession-type defects. MATERIALS AND METHODS: A total of 17 gingival recession-type defects were surgically created in the maxilla of three minipigs. The defects were randomly treated with a coronally advanced flap (CAF) and either rAmelX (test), or a CAF and placebo (control). At three months following reconstructive surgery, the animals were euthanized, and the healing outcomes histologically evaluated. RESULTS: The test group yielded statistically significantly (p = 0.047) greater formation of cementum with inserting collagen fibers compared with the control group (i.e., 4.38 mm ± 0.36 mm vs. 3.48 mm ± 1.13 mm). Bone formation measured 2.15 mm ± 0.8 mm in the test group and 2.24 mm ± 1.23 mm in the control group, respectively, without a statistically significant difference (p = 0.94). CONCLUSIONS: The present data have provided for the first-time evidence for the potential of rAmelX to promote regeneration of periodontal ligament and root cementum in recession-type defects, thus warranting further preclinical and clinical testing. CLINICAL RELEVANCE: The present results set the basis for the potential clinical application of rAmelX in reconstructive periodontal surgery. Springer Berlin Heidelberg 2023-07-08 2023 /pmc/articles/PMC10492744/ /pubmed/37421492 http://dx.doi.org/10.1007/s00784-023-05123-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Chackartchi, Tali Bosshardt, Dieter D. Imber, Jean-Claude Stähli, Alexandra Sacks, Hagit Nagy, Katalin Sculean, Anton Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
title | Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
title_full | Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
title_fullStr | Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
title_full_unstemmed | Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
title_short | Histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
title_sort | histological evaluation following treatment of recession-type defects with coronally advanced flap and a novel human recombinant amelogenin |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10492744/ https://www.ncbi.nlm.nih.gov/pubmed/37421492 http://dx.doi.org/10.1007/s00784-023-05123-x |
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